Sci. STKE, 16 November 2004
DRUG DISCOVERY Illuminating Drug Discovery
In drug discovery, profiling technologies are important for measuring drug actions both on the desired target and on other targets. High-throughput methods such as transcriptional and proteomic profiling provide data on the responses of populations of cells, normally at single-drug concentrations. Perlman et al. describe a method for high-throughput cytological profiling that combines automated microscopy, image analysis, and statistical analysis. Using multiple fluorescent probes that cover a range of cell biological functions, they profiled the dose-dependent effects of 100 drugs on cultured mammalian cells. They succeeded in categorizing drugs of known mechanism and suggested targets for drugs of uncertain mechanism.
Citation: Illuminating Drug Discovery. Sci. STKE 2004, tw416 (2004).
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