Sci. STKE, 10 May 2005
HIGH-THROUGHPUT SCREENING A Reductionist Approach in Gene Screening
Cellular signaling pathways, such as Wnt in vertebrates or Wingless in flies, have traditionally been pieced together one step at a time. Technical advances now allow a more thorough probing of the genes whose products contribute to such a regulatory system. DasGupta et al. (see the Perspective by Fearon and Cadigan and Connection Maps of the signaling pathways linked to the online paper) designed a high-throughput screen in Drosophila cells that evaluated effects on Wingless signaling when expression of nearly every gene (about 22,000 of them) was decreased, one by one, by RNA interference. The 238 genes identified included about 15 known components of the signaling pathway. The remaining group comprised approximately equal numbers of genes with known functions not previously associated with Wingless signaling. Half of the implicated genes appear to have orthologs in humans, and a substantial proportion of these human genes show mutations linked to disease.
Citation: A Reductionist Approach in Gene Screening. Sci. STKE 2005, tw177 (2005).
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