Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Sci. STKE, 10 May 2005
Vol. 2005, Issue 283, p. tw177
[DOI: 10.1126/stke.2832005tw177]


HIGH-THROUGHPUT SCREENING A Reductionist Approach in Gene Screening

Cellular signaling pathways, such as Wnt in vertebrates or Wingless in flies, have traditionally been pieced together one step at a time. Technical advances now allow a more thorough probing of the genes whose products contribute to such a regulatory system. DasGupta et al. (see the Perspective by Fearon and Cadigan and Connection Maps of the signaling pathways linked to the online paper) designed a high-throughput screen in Drosophila cells that evaluated effects on Wingless signaling when expression of nearly every gene (about 22,000 of them) was decreased, one by one, by RNA interference. The 238 genes identified included about 15 known components of the signaling pathway. The remaining group comprised approximately equal numbers of genes with known functions not previously associated with Wingless signaling. Half of the implicated genes appear to have orthologs in humans, and a substantial proportion of these human genes show mutations linked to disease.

R. DasGupta, A. Kaykas, R. T. Moon, N. Perrimon, Functional genomic analysis of the Wnt-Wingless signaling pathway. Science 308, 826-833 (2005). [Abstract] [Full Text]

E. R. Fearon, K. M. Cadigan, Wnt signaling glows with RNAi. Science 308, 801-803 (2005). [Summary] [Full Text]

Citation: A Reductionist Approach in Gene Screening. Sci. STKE 2005, tw177 (2005).

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882