Sci. STKE, 31 May 2005
BIOCHEMISTRY Proteomic Topology
The global characterizations of protein composition, either of organisms or organelles, depends on analytical techniques optimized for soluble proteins, which comprise about 70% of the coding capacity of a genome. The remaining 30% have proven more difficult to analyze as a group. Daley et al. have used topological markers [alkaline phosphatase for the periplasm and green fluorescent protein (GFP) for the cytoplasm] to establish the inside-outside orientations of the C termini of almost all of the 700 inner membrane proteins in Escherichia coli. Combining these experimental determinations with prediction algorithms based on sequence yields a much improved database of how many times each of the polypeptides crosses the membrane and of where the N and C termini are located.
Citation: Proteomic Topology. Sci. STKE 2005, tw210 (2005).
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