Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Sci. STKE, 26 July 2005
Vol. 2005, Issue 294, p. pe37
Adult Bone Marrow Stem/Progenitor Cells (MSCs) Are Preconditioned by Microenvironmental "Niches" in Culture: A Two-Stage Hypothesis for Regulation of MSC Fate
Carl A. Gregory*,
Joni Ylostalo, and
Darwin J. Prockop
Center for Gene Therapy, Tulane University Health Sciences Center, 1430 Tulane Avenue, New Orleans, LA 70112, USA.
Mesenchymal stem cells (MSCs) are clonal, plastic adherent cells from bone marrow that can differentiate into various tissue lineages, including osteoblasts, adipocytes, chondrocytes, myoblasts, hepatocytes, and possibly even neural cells. Because MSCs are multipotent and their numbers are easily expanded in culture, there has been much interest in their clinical potential for tissue repair and gene therapy. Consequently, numerous studies have been carried out demonstrating the migration and multiorgan engraftment potential of MSCs in animal models and in human clinical trials. Understanding the mechanisms behind MSC cell fate determination is not easy, because the molecular processes that drive engraftment and differentiation are complex. Even in an in vitro system, the molecular cues necessary to induce differentiation are not easily identified or reproduced. In this Perspective, we emphasize the importance of microenvironmental factors in culture and suggest that MSC differentiation in vitro is regulated by a two-stage mechanism involving preconditioning by factors in the culture microenvironment followed by response to soluble differentiating factors.
Citation: C. A. Gregory, J. Ylostalo, D. J. Prockop, Adult Bone Marrow Stem/Progenitor Cells (MSCs) Are Preconditioned by Microenvironmental "Niches" in Culture: A Two-Stage Hypothesis for Regulation of MSC Fate. Sci. STKE2005, pe37 (2005).
Activation of Human Mesenchymal Stem Cells Impacts Their Therapeutic Abilities in Lung Injury by Increasing Interleukin (IL)-10 and IL-1RN Levels.
M. L. Bustos, L. Huleihel, E. M. Meyer, A. D. Donnenberg, V. S. Donnenberg, J. D. Sciurba, L. Mroz, B. J. McVerry, B. M. Ellis, N. Kaminski, et al. (2013)
Stem Cells Trans Med
|Abstract »|Full Text »|PDF »
Keratinocyte proximity and contact can play a significant role in determining mesenchymal stem cell fate in human tissue.
Myocardial oxygenation and functional recovery in infarct rat hearts transplanted with mesenchymal stem cells.
S. M. Chacko, M. Khan, M. L. Kuppusamy, R. P. Pandian, S. Varadharaj, K. Selvendiran, A. Bratasz, B. K. Rivera, and P. Kuppusamy (2009)
Am J Physiol Heart Circ Physiol
|Abstract »|Full Text »|PDF »