Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Sci. STKE, 6 September 2005
Vol. 2005, Issue 300, p. pe43
[DOI: 10.1126/stke.3002005pe43]

PERSPECTIVES

MicroRNA-Dependent Trans-Acting siRNA Production

Hervé Vaucheret*

Laboratoire de Biologie Cellulaire, Institut Jean-Pierre Bourgin, INRA 78026 Versailles Cedex, France.

Abstract: Less than a year has elapsed between the discovery of trans-acting small interfering RNAs (tasiRNAs) in plants and the elucidation of the major steps of the corresponding pathway. During tasiRNA biogenesis, polyadenylated RNAs transcribed from non–protein-coding TAS genes are cleaved by a microRNA (miRNA)–programmed RNA-induced silencing complex. In contrast to classical miRNA targets, RDR6 and SGS3 convert one of the TAS RNA cleavage products into double-stranded RNA, which is subsequently processed, in a phase determined by the initial miRNA cleavage site, by DICER-LIKE 4 to generate a 21-nucleotide tasiRNA population. tasiRNAs guide endogenous mRNA cleavage through the action of AGO1 or, perhaps in some cases, AGO7. Some of the tasiRNA targets probably regulate the juvenile-to-adult phase transition, but the roles of other tasiRNA targets remain to be determined.

*Contact information. E-mail, herve.vaucheret{at}versailles.inra.fr

Citation: H. Vaucheret, MicroRNA-Dependent Trans-Acting siRNA Production. Sci. STKE 2005, pe43 (2005).

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Differential effects of viral silencing suppressors on siRNA and miRNA loading support the existence of two distinct cellular pools of ARGONAUTE1.
G. Schott, A. Mari-Ordonez, C. Himber, A. Alioua, O. Voinnet, and P. Dunoyer (2012)
EMBO J. 31, 2553-2565
   Abstract »    Full Text »    PDF »
Identifying small interfering RNA loci from high-throughput sequencing data.
T. J. Hardcastle, K. A. Kelly, and D. C. Baulcombe (2012)
Bioinformatics 28, 457-463
   Abstract »    Full Text »    PDF »
22-nucleotide RNAs trigger secondary siRNA biogenesis in plants.
H.-M. Chen, L.-T. Chen, K. Patel, Y.-H. Li, D. C. Baulcombe, and S.-H. Wu (2010)
PNAS 107, 15269-15274
   Abstract »    Full Text »    PDF »
An endogenous, systemic RNAi pathway in plants.
P. Dunoyer, C. A. Brosnan, G. Schott, Y. Wang, F. Jay, A. Alioua, C. Himber, and O. Voinnet (2010)
EMBO J. 29, 1699-1712
   Abstract »    Full Text »    PDF »
Distinct extremely abundant siRNAs associated with cosuppression in petunia.
E. De Paoli, A. Dorantes-Acosta, J. Zhai, M. Accerbi, D.-H. Jeong, S. Park, B. C. Meyers, R. A. Jorgensen, and P. J. Green (2009)
RNA 15, 1965-1970
   Abstract »    Full Text »    PDF »
Genome-Wide Analysis of the RNA-DEPENDENT RNA POLYMERASE6/DICER-LIKE4 Pathway in Arabidopsis Reveals Dependency on miRNA- and tasiRNA-Directed Targeting.
M. D. Howell, N. Fahlgren, E. J. Chapman, J. S. Cumbie, C. M. Sullivan, S. A. Givan, K. D. Kasschau, and J. C. Carrington (2007)
PLANT CELL 19, 926-942
   Abstract »    Full Text »    PDF »
Distinct Populations of Primary and Secondary Effectors During RNAi in C. elegans.
J. Pak and A. Fire (2007)
Science 315, 241-244
   Abstract »    Full Text »    PDF »
Rapid Metabolism of Glucose Detected with FRET Glucose Nanosensors in Epidermal Cells and Intact Roots of Arabidopsis RNA-Silencing Mutants.
K. Deuschle, B. Chaudhuri, S. Okumoto, I. Lager, S. Lalonde, and W. B. Frommer (2006)
PLANT CELL 18, 2314-2325
   Abstract »    Full Text »    PDF »

To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882