Sci. STKE, 6 December 2005
CELL DIVISION Keeping Survivin on Target
Many proteins are involved in the orchestration of the events required for the successful completion of mitosis. Vong et al. (see the Perspective by Earnshaw) searched for proteins that interact with survivin, a protein that functions in the regulation of mitosis and accumulates on condensing chromosomes at the centromeres and later on the spindle. A deubiquitinating enzyme known as hFAM was identified that appears to control localization of survivin and its association with other proteins. Survivin was coupled to ubiquitin through Lys63 linkages, a modification that influences protein-protein interactions, and this modification was necessary for proper binding of survivin to centromeres. Preventing ubiquitination by mutating Lys63 disrupted chromosome alignments and mitotic progression. Thus, enzymes regulating protein ubiquitination appear to have key regulatory roles in controlling the dynamic protein interactions required for proper execution of mitosis.
Citation: Keeping Survivin on Target. Sci. STKE 2005, tw440 (2005).
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