Sci. STKE, 9 January 2007
Chemistry Cellular Accounting
Philip D. Szuromi
Science, AAAS, Washington, DC 20005, USA
Fluorescent labeling has allowed quantification of proteins in single cells, but potential problems arise from interference between reporter molecules themselves and interactions with other molecules within the cell. Huang et al. have devised a microfluidic chip for counting fluorescent proteins within a cell. Single cells are captured and lysed, and their contents are separated by electrophoresis and quantified by single-molecule fluorescence detection. This method was applied to natively fluorescent compounds (the phycobiliprotein subcomplexes in individual cyanobacterial cells grown under nitrogen-limited conditions), and proteins were labeled with fluorescent antibodies (2 adrenergic receptor) present in low copy numbers.
Citation: P. D. Szuromi, Cellular Accounting. Sci. STKE 2007, tw16 (2007).
Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882