Sci. STKE, 9 January 2007
Cell Biology Enzyme Kinetics in Living Color
Stella M. Hurtley
Science, AAAS, Cambridge CB2 1LQ, UK
Innovative methods are required to study the spatial regulation of enzymatic activity inside living cells. Yudushkin et al. describe such a method based on the detection of enzyme-substrate complexes using fluorescence lifetime imaging microscopy. The detection of the enzyme interacting with the substrate ensures utmost specificity and enables evaluation of the localized activity of a particular enzymatic species. The technique was used to investigate the spatial regulation of growth factor signaling by the tyrosine phosphatase PTP1B and revealed that PTP1B exists inside cells as kinetically distinct, spatially separated subpopulations.
I. A. Yudushkin, A. Schleifenbaum, A. Kinkhabwala, B. G. Neel, C. Schultz, P. I. H. Bastiaens, Live-cell imaging of enzyme-substrate interaction reveals spatial regulation of PTP1B. Science 315, 115-119 (2007). [Abstract] [Full Text]
Citation: S. M. Hurtley, Enzyme Kinetics in Living Color. Sci. STKE 2007, tw17 (2007).
Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882