Sci. STKE, 1 May 2007
Cell Biology Life Without Dynamin
Stella M. Hurtley
Science, AAAS, Cambridge CB2 1LQ, UK
Dynamin 1 is a neuron-specific guanosine triphosphatase involved in the endocytic recycling of synaptic vesicle membranes. Ferguson et al. created genetically engineered mice lacking dynamin 1 and found, surprisingly, that they contained functional synapses and had limited postnatal viability. However, the synapses of these dynamin 1 knockouts contained branched, tubular plasma membrane invaginations capped by clathrin-coated pits, consistent with dynamin 1s proposed role in clathrin-coated vesicle scission. Also, after strong stimulation, synaptic vesicle endocytosis was severely impaired but could resume efficiently upon stimulus termination. This finding reveals the existence of a dynamin 1-independent mechanism that can support limited synaptic vesicle endocytosis.
S. M. Ferguson, G. Brasnjo, M. Hayashi, M. Wölfel, C. Collesi, S. Giovedi, A. Raimondi, L.-W. Gong, P. Ariel, S. Paradise, E. OToole, R. Flavell, O. Cremona, G. Miesenböck, T. A. Ryan, P. De Camilli, A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis. Science 316, 570-574 (2007). [Abstract] [Full Text]
Citation: S. M. Hurtley, Life Without Dynamin. Sci. STKE 2007, tw155 (2007).
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