Sci. STKE, 12 June 2007
Molecular Biology Target Identification Tool
Science, AAAS, Washington, DC 20005, USA
A challenge for functional genomics has been to make meaningful global measurements of the interactions between transcription factors (and cofactors) and DNA. It has been difficult, especially in large genomes, to explicitly map individual binding sites and individual factor-target gene interactions. Johnson et al. (see the Perspective by Fields) have developed a combination of chromatin immunoprecipitation and ultrahigh-throughput sequencing to achieve high specificity and 50-base pair resolution. This approach was used to study regulation by neuron-restrictive silencer factor (NRSF, also known as REST, for repressor element-1 silencing transcription factor) and identify targets of key positive regulators of pancreatic neuroendocrine development.
Citation: B. Jasny, Target Identification Tool. Sci. STKE 2007, tw210 (2007).
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