Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Subscribe

Sci. STKE, 10 July 2007
Vol. 2007, Issue 394, p. tr2
[DOI: 10.1126/stke.3942007tr2]

TEACHING RESOURCES

A Biochemical Assay for Acetylcholinesterase Activity in PC12 Cells

Paul J. Schwartz1, Jay A. Blundon2, and Elizabeth M. Adler3*

1Department of Neurological Surgery, University of Medicine and Dentistry of New Jersey, Newark, NJ 07101, USA.
2Department of Biology, Rhodes College, Memphis, TN 38112, USA.
3Associate Editor of Science’s STKE, American Association for the Advancement of Science, 1200 New York Avenue, NW, Washington, DC 20005, USA.

Abstract: This lab describes two biochemical assays: One for measuring acetylcholinesterase activity and one for measuring protein concentration. Students learn how to manipulate small-volume samples, use a standard spectrophotometer or a microplate reader spectrophotometer, construct a standard curve, and normalize data. The lab is intended to be used in conjunction with a cell culture lab in which PC12 cells are exposed to various agents that influence their phenotypic state.

*Corresponding author. E-mail, eadler{at}aaas.org

Citation: P. J. Schwartz, J. A. Blundon, E. M. Adler, A Biochemical Assay for Acetylcholinesterase Activity in PC12 Cells. Sci. STKE 2007, tr2 (2007).

Read the Full Text

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882