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Sci. STKE, 10 July 2007
[DOI: 10.1126/stke.3942006tr4]

TEACHING RESOURCES

Introduction to Enzyme Kinetics: Assay of beta-Galactosidase

Joanne Kivela Tillotson*

Purchase College, State University of New York

Abstract: This tutorial describes a colorometric enzyme assay to determine simple enzyme kinetics. It is designed as an introduction for beginning laboratory students in a biology or biochemistry course, and requires no previous understanding of chemistry. It may be used as an assignment to prepare students for a laboratory exercise in enzyme kinetics Through this tutorial, students will gain an understanding of the procedures for measuring enzyme activity, using the specific example of an assay for the bacterial enzyme, beta-galactosidase.

*Contact information. E-mail, joanne.tillotson{at}purchase.edu

Citation: J. K. Tillotson, Introduction to Enzyme Kinetics: Assay of beta-Galactosidase. Sci. STKE 2007, tr4 (2007).

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Science Signaling. ISSN 1937-9145 (pre-2008: Science's STKE. ISSN 1525-8882)