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Sci. STKE, 4 September 2007
Vol. 2007, Issue 402, p. tw320
[DOI: 10.1126/stke.4022007tw320]

EDITORS' CHOICE

Neuroscience TNF and Alzheimer’s Disease

John F. Foley

Science’s STKE, AAAS, Washington, DC 20005, USA

Alzheimer’s disease (AD) is associated with the accumulation in the brain of plaques of amyloid beta protein (Abeta), which is produced by the processing of amyloid precursor protein (APP) by the enzymes beta-secretase (BACE1) and {gamma}-secretase. He et al. pursued their earlier finding that overexpression of tumor necrosis factor (TNF) receptor 1 (TNFR1) promoted Abeta-induced neuronal death by examining the role of TNFR1 in Abeta production. They crossed transgenic APP23 mice, which express a mutant APP and develop Abeta plaques, with TNFR1–/– mice (APP23/TNFR1–/–). Immunhistochemical analyses of the brains of 14-month-old animals showed a decreased number and size of Abeta plaques in APP23/TNFR1–/– mice compared with those in APP23 mice. Real-time reverse transcription polymerase chain reaction assays, together with Western blotting, showed that the abundance of BACE1 mRNA and protein were lower in APP23/TNFR1–/– mice compared with APP23 mice, which suggests that TNFR1 signaling promotes the production of BACE1. Experiments in 293 cells transfected with a reporter plasmid showed that TNF-{alpha} treatment induced BACE1 promoter activity, which was blocked by a specific inhibitor of the transcription factor nuclear factor {kappa}B (NF-{kappa}B). Immunohistochemistry demonstrated the presence of fewer neurons in the brains of APP23 mice than in brains from APP23/TNFR1–/– mice. In learning and object recognition tests, APP23 mice performed poorly; APP23/TNFR1–/– mice performed better, with abilities similar to those of wild-type mice. Together, these data implicate TNFR1 in promoting abnormal APP processing, Abeta plaque formation, and learning defects and suggest that TNFR1 might be an effective therapeutic target in treating AD.

P. He, Z. Zhong, K. Lindholm, L. Berning, W. Lee, C. Lemere, M. Staufenbiel, R. Li, Y. Shen, Deletion of tumor necrosis factor death receptor inhibits amyloid beta generation and prevents learning and memory deficits in Alzheimer’s mice. J. Cell Biol. 178, 829-841 (2007). [Abstract] [Full Text]

Citation: J. F. Foley, TNF and Alzheimer’s Disease. Sci. STKE 2007, tw320 (2007).



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