Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Sci. STKE, 20 November 2007
Vol. 2007, Issue 413, p. tw426
[DOI: 10.1126/stke.4132007tw426]


Cell Adhesion Remembering the Last Engagement

Elizabeth M. Adler

Science's STKE, AAAS, Washington, DC 20005, USA

The experimental analysis of single-molecule biomechanical interactions frequently involves sequentially repeated measurements that are used to obtain a sufficiently large amount of data. The validity of this approach depends on the assumption that such sequential measurements are identical and independent of each other, an assumption Zarnitsyna et al. tested using a micropipette adhesion frequency assay. Cells expressing a particular ligand were held in a micropipette, and their adhesion (or lack thereof) to a second cell expressing the cognate receptor was repeatedly determined after an identical period of contact. Analyses of different receptor-ligand pairs revealed three distinct behaviors. The interaction between lymphocyte function-associated antigen-1 (LFA-1) and intercellular adhesion molecule-1 (ICAM-1) was consistent with the assumption of independent and identical measurements. In contrast, interactions between the T cell receptor (TCR) and an antigen peptide bound to a major histocompatibility complex (pMHC) or between C-cadherins appeared to show molecular "memory." Thus, adhesion between a TCR-pMHC pair in one test increased the probability of adhesion in the next test, whereas adhesion between C-cadherins in one test decreased the likelihood that they would adhere in the next. Thus, the authors propose that receptor-ligand interactions involved in mediating cell adhesion show molecular memory, likely mediated through responses triggered by receptor engagement.

V. I. Zarnitsyna, J. Huang, F. Zhang, Y.-H. Chien, D. Leckband, C. Zhu, Memory in receptor-ligand-mediated cell adhesion. Proc. Natl. Acad. Sci. U.S.A. 104, 18037-18042 (2007). [Abstract] [Full Text]

Citation: E. M. Adler, Remembering the Last Engagement. Sci. STKE 2007, tw426 (2007).

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882