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Sci. STKE, 18 December 2007
Vol. 2007, Issue 417, p. pl7
[DOI: 10.1126/stke.4172007pl7]

PROTOCOLS

Image Correlation Spectroscopy

Anja Nohe1* and Nils O. Petersen2

1Department of Chemical and Biological Engineering, University of Maine, Orono, ME 04469, USA.
2National Institute for Nanotechnology, University of Alberta, 11421 Saskatchewan Drive, Edmonton, AB T6G 2M9, Canada.

Abstract: Membrane domains, such as caveolae and clathrin-coated pits, regulate cell signaling and protein internalization in the plasma membrane. Fluorescence imaging and microscopy provide an opportunity to determine the receptor protein dynamics of membrane microdomains. The family of image correlation spectroscopy (ICS) techniques provides powerful tools with which to measure the aggregation, clustering, and dynamics of proteins in the plasma membrane. ICS is used to calculate the cluster density and the degree of aggregation of plasma membrane proteins, whereas image cross-correlation spectroscopy (ICCS) measures the fraction of colocalization of two proteins. Dynamic image correlation spectroscopy (DICS) can be used to analyze protein dynamics on the cell surface during live-cell imaging.

*Corresponding author. E-mail, anohe{at}umche.maine.edu

Citation: A. Nohe, N. O. Petersen, Image Correlation Spectroscopy. Sci. STKE 2007, pl7 (2007).

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