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Sci. Signal., 23 February 2010
Vol. 3, Issue 110, p. ra14
[DOI: 10.1126/scisignal.2000467]


DNA-PKcs Controls an Endosomal Signaling Pathway for a Proinflammatory Response by Natural Killer Cells

Sumati Rajagopalan1*, Mark W. Moyle1, Irma Joosten2, and Eric O. Long1*

1 Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA.
2 Department of Laboratory Medicine, Radboud University Nijmegen Medical Center, 6500 HB Nijmegen, the Netherlands.

Abstract: Endosomes are emerging as specialized signaling compartments that endow receptors with distinct signaling properties. The diversity of endosomal signaling pathways and their contribution to various biological responses is still unclear. CD158d, which is also known as the killer cell immunoglobulin-like receptor (KIR) 2DL4 (KIR2DL4), is an endosome-resident receptor in natural killer (NK) cells that stimulates the release of a unique set of proinflammatory and proangiogenic mediators in response to soluble human leukocyte antigen G (HLA-G). Here, we identified the CD158d signaling cascade. In response to soluble agonist antibody or soluble HLA-G, signaling by CD158d was dependent on the activation of nuclear factor {kappa}B (NF-{kappa}B) and the serine-threonine kinase Akt. CD158d associated with the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs), promoted the recruitment of Akt to endosomes, and stimulated the DNA-PKcs–dependent phosphorylation of Akt. The sequential requirement for DNA-PKcs, Akt, and NF-{kappa}B in signaling by CD158d delineates a previously uncharacterized endosomal signaling pathway for a proinflammatory response in NK cells.

* To whom correspondence should be addressed. E-mail: sumi{at} (S.R.) and eLong{at} (E.O.L.)

Citation: S. Rajagopalan, M. W. Moyle, I. Joosten, E. O. Long, DNA-PKcs Controls an Endosomal Signaling Pathway for a Proinflammatory Response by Natural Killer Cells. Sci. Signal. 3, ra14 (2010).

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