Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Sci. Signal., 27 July 2010
Vol. 3, Issue 132, p. ra57
[DOI: 10.1126/scisignal.2000740]


The Drosophila Female Germline Stem Cell Lineage Acts to Spatially Restrict DPP Function Within the Niche

Ming Liu1,2, Tit Meng Lim2, and Yu Cai1,2*

1 Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117604, Singapore.
2 Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore.

Abstract: Maintenance of stem cells requires spatially restricted, niche-associated signals. In the Drosophila female germline stem cell (GSC) niche, Decapentaplegic (DPP) is the primary niche-associated factor and functions over a short range to promote GSC self-renewal rather than differentiation. Here, we show that the GSC lineage and, more specifically, the stem cells themselves participate in the spatial restriction of DPP function by activating epidermal growth factor receptor (EGFR)–mitogen-activated protein kinase (MAPK) signaling in the surrounding somatic cells. EGFR-MAPK signaling in somatic cells repressed the expression of dally, which encodes a glypican required for DPP movement and stability. Consequently, only GSCs close to the DPP source (the somatic cells in the niche) showed high signal activation and were maintained as stem cells, whereas cystoblasts outside the niche showed low signal activation and initiated differentiation. Thus, our data reveal that the reciprocal crosstalk between the GSCs and the somatic cells defines the spatial limits of DPP action and therefore the extent of the GSC niche.

* To whom correspondence should be addressed. E-mail: caiyu{at}

Citation: M. Liu, T. M. Lim, Y. Cai, The Drosophila Female Germline Stem Cell Lineage Acts to Spatially Restrict DPP Function Within the Niche. Sci. Signal. 3, ra57 (2010).

Read the Full Text

Diet controls Drosophila follicle stem cell proliferation via Hedgehog sequestration and release.
T. R. Hartman, T. I. Strochlic, Y. Ji, D. Zinshteyn, and A. M. O'Reilly (2013)
J. Cell Biol. 201, 741-757
   Abstract »    Full Text »    PDF »
Histone demethylase dUTX antagonizes JAK-STAT signaling to maintain proper gene expression and architecture of the Drosophila testis niche.
L. Tarayrah, H.-M. Herz, A. Shilatifard, and X. Chen (2013)
Development 140, 1014-1023
   Abstract »    Full Text »    PDF »
Self-maintained escort cells form a germline stem cell differentiation niche.
D. Kirilly, S. Wang, and T. Xie (2011)
Development 138, 5087-5097
   Abstract »    Full Text »    PDF »
Cease and desist: modulating short-range Dpp signalling in the stem-cell niche.
R. E. Harris and H. L. Ashe (2011)
EMBO Rep. 12, 519-526
   Abstract »    Full Text »    PDF »
Loss of lysine-specific demethylase 1 nonautonomously causes stem cell tumors in the Drosophila ovary.
S. Eliazer, N. A. Shalaby, and M. Buszczak (2011)
PNAS 108, 7064-7069
   Abstract »    Full Text »    PDF »

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882