Sci. Signal., 15 February 2011
Cell Biology More or Less Durable
Stella M. Hurtley
Science, AAAS, Cambridge CB2 1LQ, UK
The ability to measure protein half-lives on a large scale in human cells should contribute to our understanding of a variety of physiological and pathological processes. To meet this goal, Eden et al. (see the Perspective by Plotkin) developed a method called bleach-chase. Bleach-chase was used to reveal an unexpectedly simple response of fluorescently tagged protein half-lives to stresses and to drugs that stop cell division: Long-lived proteins become longer-lived. It appears that changes in cell growth cause changes in the intracellular dilution rates of proteins, which are not balanced by corresponding changes in active protein degradation.
Citation: S. M. Hurtley, More or Less Durable. Sci. Signal. 4, ec48 (2011).
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