Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Sci. Signal., 17 May 2011
Vol. 4, Issue 173, p. ra32
[DOI: 10.1126/scisignal.2001346]

RESEARCH ARTICLES

Stomatal Closure by Fast Abscisic Acid Signaling Is Mediated by the Guard Cell Anion Channel SLAH3 and the Receptor RCAR1

Dietmar Geiger1*, Tobias Maierhofer1*, Khaled A. S. AL-Rasheid2, Sönke Scherzer1, Patrick Mumm1, Anja Liese3, Peter Ache1, Christian Wellmann4, Irene Marten1, Erwin Grill4, Tina Romeis3, and Rainer Hedrich1{dagger}

1 Institute for Molecular Plant Physiology and Biophysics, University Würzburg, Julius-von-Sachs Platz 2, D-97082 Würzburg, Germany.
2 Zoology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
3 Department of Plant Biochemistry, Free University Berlin, Koenigin-Luise-Strasse 12-16, D-14195 Berlin, Germany.
4 Lehrstuhl für Botanik, Technische Universität München, Am Hochanger 4, D-85354 Freising, Germany.

* These authors contributed equally to this work.

Abstract: S-type anion channels are direct targets of abscisic acid (ABA) signaling and contribute to chloride and nitrate release from guard cells, which in turn initiates stomatal closure. SLAC1 was the first component of the guard cell S-type anion channel identified. However, we found that guard cells of Arabidopsis SLAC1 mutants exhibited nitrate conductance. SLAH3 (SLAC1 homolog 3) was also present in guard cells, and coexpression of SLAH3 with the calcium ion (Ca2+)–dependent kinase CPK21 in Xenopus oocytes mediated nitrate-induced anion currents. Nitrate, calcium, and phosphorylation regulated SLAH3 activity. CPK21-dependent SLAH3 phosphorylation and activation were blocked by ABI1, a PP2C-type protein phosphatase that is inhibited by ABA and inhibits the ABA signaling pathway in guard cells. We reconstituted the ABA-stimulated phosphorylation of the SLAH3 amino-terminal domain by CPK21 in vitro by including the ABA receptor–phosphatase complex RCAR1-ABI1 in the reactions. We propose that ABA perception by the complex consisting of ABA receptors of the RCAR/PYR/PYL family and ABI1 releases CPK21 from inhibition by ABI1, and then CPK21 is further activated by an increase in the cytosolic Ca2+ concentration, leading to its phosphorylation of SLAH3. Thus, the identification of SLAH3 as the nitrate-, calcium-, and ABA-sensitive guard cell anion channel provides insights into the relationship among stomatal response to drought, signaling by nitrate, and nitrate metabolism.

{dagger} To whom correspondence should be addressed: E-mail: hedrich{at}botanik.uni-wuerzburg.de

Citation: D. Geiger, T. Maierhofer, K. A. S. AL-Rasheid, S. Scherzer, P. Mumm, A. Liese, P. Ache, C. Wellmann, I. Marten, E. Grill, T. Romeis, R. Hedrich, Stomatal Closure by Fast Abscisic Acid Signaling Is Mediated by the Guard Cell Anion Channel SLAH3 and the Receptor RCAR1. Sci. Signal. 4, ra32 (2011).

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Mechano-Stimulation Triggers Turgor Changes Associated with Trap Closure in the Darwin Plant Dionaea muscipula.
M. Escalante-Perez, S. Scherzer, K. A. S. Al-Rasheid, C. Dottinger, E. Neher, and R. Hedrich (2014)
Mol Plant 7, 744-746
   Full Text »    PDF »
Separating Active and Passive Influences on Stomatal Control of Transpiration[OPEN].
S. A. M. McAdam and T. J. Brodribb (2014)
Plant Physiology 164, 1578-1586
   Abstract »    Full Text »    PDF »
Nitrate transport and signalling in Arabidopsis.
A. Krapp, L. C. David, C. Chardin, T. Girin, A. Marmagne, A.-S. Leprince, S. Chaillou, S. Ferrario-Mery, C. Meyer, and F. Daniel-Vedele (2014)
J. Exp. Bot. 65, 789-798
   Abstract »    Full Text »    PDF »
Foliar abscisic acid content underlies genotypic variation in stomatal responsiveness after growth at high relative air humidity.
H. Giday, D. Fanourakis, K. H. Kjaer, I. S. Fomsgaard, and C.-O. Ottosen (2013)
Ann. Bot. 112, 1857-1867
   Abstract »    Full Text »    PDF »
Pollen Tube Growth Regulation by Free Anions Depends on the Interaction between the Anion Channel SLAH3 and Calcium-Dependent Protein Kinases CPK2 and CPK20.
T. Gutermuth, R. Lassig, M.-T. Portes, T. Maierhofer, T. Romeis, J.-W. Borst, R. Hedrich, J. A. Feijo, and K. R. Konrad (2013)
PLANT CELL 25, 4525-4543
   Abstract »    Full Text »    PDF »
Calcium-Dependent Protein Kinases: Hubs in Plant Stress Signaling and Development.
P. Schulz, M. Herde, and T. Romeis (2013)
Plant Physiology 163, 523-530
   Full Text »    PDF »
Calcium-Dependent Protein Kinase CPK6 Positively Functions in Induction by Yeast Elicitor of Stomatal Closure and Inhibition by Yeast Elicitor of Light-Induced Stomatal Opening in Arabidopsis.
W. Ye, D. Muroyama, S. Munemasa, Y. Nakamura, I. C. Mori, and Y. Murata (2013)
Plant Physiology 163, 591-599
   Abstract »    Full Text »    PDF »
C-Terminus-Mediated Voltage Gating of Arabidopsis Guard Cell Anion Channel QUAC1.
P. Mumm, D. Imes, E. Martinoia, K. A. S. Al-Rasheid, D. Geiger, I. Marten, and R. Hedrich (2013)
Mol Plant 6, 1550-1563
   Abstract »    Full Text »    PDF »
Salt Stress Triggers Phosphorylation of the Arabidopsis Vacuolar K+ Channel TPK1 by Calcium-Dependent Protein Kinases (CDPKs).
A. Latz, N. Mehlmer, S. Zapf, T. D. Mueller, B. Wurzinger, B. Pfister, E. Csaszar, R. Hedrich, M. Teige, and D. Becker (2013)
Mol Plant 6, 1274-1289
   Abstract »    Full Text »    PDF »
PYR/RCAR Receptors Contribute to Ozone-, Reduced Air Humidity-, Darkness-, and CO2-Induced Stomatal Regulation.
E. Merilo, K. Laanemets, H. Hu, S. Xue, L. Jakobson, I. Tulva, M. Gonzalez-Guzman, P. L. Rodriguez, J. I. Schroeder, M. Brosche, et al. (2013)
Plant Physiology 162, 1652-1668
   Abstract »    Full Text »    PDF »
bHLH Transcription Factors That Facilitate K+ Uptake During Stomatal Opening Are Repressed by Abscisic Acid Through Phosphorylation.
Y. Takahashi, Y. Ebisu, T. Kinoshita, M. Doi, E. Okuma, Y. Murata, and K.-i. Shimazaki (2013)
Science Signaling 6, ra48
   Abstract »    Full Text »    PDF »
Calcium-dependent protein kinase/NADPH oxidase activation circuit is required for rapid defense signal propagation.
U. Dubiella, H. Seybold, G. Durian, E. Komander, R. Lassig, C.-P. Witte, W. X. Schulze, and T. Romeis (2013)
PNAS 110, 8744-8749
   Abstract »    Full Text »    PDF »
Arabidopsis nanodomain-delimited ABA signaling pathway regulates the anion channel SLAH3.
F. Demir, C. Horntrich, J. O. Blachutzik, S. Scherzer, Y. Reinders, S. Kierszniowska, W. X. Schulze, G. S. Harms, R. Hedrich, D. Geiger, et al. (2013)
PNAS 110, 8296-8301
   Abstract »    Full Text »    PDF »
Reconstitution of abscisic acid activation of SLAC1 anion channel by CPK6 and OST1 kinases and branched ABI1 PP2C phosphatase action.
B. Brandt, D. E. Brodsky, S. Xue, J. Negi, K. Iba, J. Kangasjarvi, M. Ghassemian, A. B. Stephan, H. Hu, and J. I. Schroeder (2012)
PNAS 109, 10593-10598
   Abstract »    Full Text »    PDF »
Fern and Lycophyte Guard Cells Do Not Respond to Endogenous Abscisic Acid.
S. A. M. McAdam and T. J. Brodribb (2012)
PLANT CELL 24, 1510-1521
   Abstract »    Full Text »    PDF »
Disruption of the Arabidopsis thaliana Inward-Rectifier K+ Channel AKT1 Improves Plant Responses to Water Stress.
M. Nieves-Cordones, F. Caballero, V. Martinez, and F. Rubio (2012)
Plant Cell Physiol. 53, 423-432
   Abstract »    Full Text »    PDF »
Selective Inhibition of Clade A Phosphatases Type 2C by PYR/PYL/RCAR Abscisic Acid Receptors.
R. Antoni, M. Gonzalez-Guzman, L. Rodriguez, A. Rodrigues, G. A. Pizzio, and P. L. Rodriguez (2012)
Plant Physiology 158, 970-980
   Abstract »    Full Text »    PDF »
Abscisic acid and CO2 signalling via calcium sensitivity priming in guard cells, new CDPK mutant phenotypes and a method for improved resolution of stomatal stimulus-response analyses.
K. E. Hubbard, R. S. Siegel, G. Valerio, B. Brandt, and J. I. Schroeder (2012)
Ann. Bot. 109, 5-17
   Abstract »    Full Text »    PDF »
A Brand New START: Abscisic Acid Perception and Transduction in the Guard Cell.
A. Joshi-Saha, C. Valon, and J. Leung (2011)
Science Signaling 4, re4
   Abstract »    Full Text »    PDF »
A thermodynamic switch modulates abscisic acid receptor sensitivity.
F. Dupeux, J. Santiago, K. Betz, J. Twycross, S.-Y. Park, L. Rodriguez, M. Gonzalez-Guzman, M. R. Jensen, N. Krasnogor, M. Blackledge, et al. (2011)
EMBO J. 30, 4171-4184
   Abstract »    Full Text »    PDF »

To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882