Phosphoproteomics Sorting Out the Centrosome

L. Bryan Ray

Science, Science Signaling, AAAS, Washington, DC 20005, USA

The centrosome is the paired organelle that organizes microtubules to form the mitotic spindle. The yeast centrosome (called the spindle pole body) is composed of 18 proteins. To help better understand how the centrosome is regulated, Keck et al. made a comprehensive analysis of phosphorylation of the yeast centrosome proteins by mass spectroscopy. Almost 300 sites of phosphorylation were identified, about 100 of which occurred only during mitosis. The results may help point the way for further functional characterization of the more complicated human centrosome, which, with some 100 proteins, is likely to be regulated by a very large set of phosphorylation events.

J. M. Keck, M. H. Jones, C. C. L. Wong, J. Binkley, D. Chen, S. L. Jaspersen, E. P. Holinger, T. Xu, M. Niepel, M. P. Rout, J. Vogel, A. Sidow, J. R. Yates III, M. Winey, A cell cycle phosphoproteome of the yeast centrosome. Science 332, 1557–1561 (2011). [Abstract] [Full Text]

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PODCASTS
Phosphoproteomics
Science Signaling Podcast: 28 June 2011

Silke Hauf, Mark Winey, Michele H. Jones, Jamie M. Keck, and Annalisa M. VanHook (28 June 2011)
Sci. Signal. 4 (179), pc12. [DOI: 10.1126/scisignal.2002245]
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RESEARCH RESOURCES
Phosphoproteomics
Mitotic Substrates of the Kinase Aurora with Roles in Chromatin Regulation Identified Through Quantitative Phosphoproteomics of Fission Yeast

André Koch, Karsten Krug, Stuart Pengelley, Boris Macek, and Silke Hauf (28 June 2011)
Sci. Signal. 4 (179), rs6. [DOI: 10.1126/scisignal.2001588]
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