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Sci. Signal., 13 September 2011
Vol. 4, Issue 190, p. ra59
[DOI: 10.1126/scisignal.2001893]

RESEARCH ARTICLES

Feedback Circuits Monitor and Adjust Basal Lck-Dependent Events in T Cell Receptor Signaling

Jamie R. Schoenborn1*, Ying Xim Tan1, Chao Zhang2, Kevan M. Shokat2,3, and Arthur Weiss1,3{dagger}

1 Rosalind Russell Medical Research Center for Arthritis, Division of Rheumatology, Department of Medicine, University of California, San Francisco, CA 94143, USA.
2 Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94143, USA.
3 Howard Hughes Medical Institute.

* Present address: Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.

Abstract: The Src family kinase Lck is crucial for the initiation of TCR signaling. The activity of Lck is tightly controlled to prevent erroneous immune activation, yet it enables rapid cellular responses over a range of sensitivities to antigens. Here, in experiments with an analog-sensitive variant of the tyrosine kinase Csk, we report that Lck in T cells is dynamically controlled by an equilibrium between Csk and the tyrosine phosphatase CD45. By rapidly inhibiting Csk, we showed that changes in this equilibrium were sufficient to activate canonical TCR signaling pathways independently of ligand binding to the TCR. The activated signaling pathways showed sustained and enhanced phosphorylation compared to that in TCR-stimulated cells, revealing a feedback circuit that was sensitive to the basal signaling machinery. We identified the inhibitory adaptor molecule Dok-1 (downstream of kinase 1) as a candidate that may respond to alterations in basal signaling activity. Our results also suggest a role for Csk in the termination or dampening of TCR signals.

{dagger} To whom correspondence should be addressed. E-mail: aweiss{at}medicine.ucsf.edu

Citation: J. R. Schoenborn, Y. X. Tan, C. Zhang, K. M. Shokat, A. Weiss, Feedback Circuits Monitor and Adjust Basal Lck-Dependent Events in T Cell Receptor Signaling. Sci. Signal. 4, ra59 (2011).

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