Protein Complexes and Target Genes Identification by in Vivo Biotinylation: The STAT5 Paradigm

Eleni Katsantoni^*

Hematology/Oncology Division, Biomedical Research Foundation, Academy of Athens, 11527 Athens, Greece.

A Presentation from the European Society for Paediatric Endocrinology (ESPE) New Inroads to Child Health (NICHe) Conference on Stress Response and Child Health in Heraklion, Crete, Greece, 18 to 20 May 2012.

Abstract: Signal transducers and activators of transcription (STATs) are implicated in physiological cellular processes, stress-related responses, and malignancies. This Presentation provides an overview on STATs, focusing on STAT5, and describes an in vivo biotinylation methodology applied in mammalian cells for identification of STAT5-containing protein complexes and STAT5 target genes. Our results show that in vivo biotinylation can be used efficiently to elucidate the complex STAT5-dependent transcriptional mechanisms.

* Presenter and corresponding author. E-mail: ekatsantoni{at}bioacademy.gr

The editors suggest the following Related Resources on Science sites:

In Science Signaling

ERRATA
A Correction to the Presentation Titled: "Protein Complexes and Target Genes Identification by in Vivo Biotinylation: The STAT5 Paradigm" by E. Katsantoni

(27 November 2012)
Sci. Signal. 5 (252), er7. [DOI: 10.1126/scisignal.5252er7]
 |  Full Text »  |  PDF »

PRESENTATIONS
Endocrinology
Prenatal Stress, Telomere Biology, and Fetal Programming of Health and Disease Risk

Sonja Entringer, Claudia Buss, and Pathik D. Wadhwa (30 October 2012)
Sci. Signal. 5 (248), pt12. [DOI: 10.1126/scisignal.2003580]
 |  Abstract »  |  Full Text »  |  PDF »  |  Slideshow »

MEETING REPORTS
Endocrinology
Stress Response and Child Health

Evangelia Charmandari, John C. Achermann, Jean-Claude Carel, Olle Soder, and George P. Chrousos (30 October 2012)
Sci. Signal. 5 (248), mr1. [DOI: 10.1126/scisignal.2003595]
 |  Abstract »  |  Full Text »  |  PDF »

PRESENTATIONS
Endocrinology
P450 Oxidoreductase Deficiency: A Disorder of Steroidogenesis with Multiple Clinical Manifestations

Walter L. Miller (23 October 2012)
Sci. Signal. 5 (247), pt11. [DOI: 10.1126/scisignal.2003318]
 |  Abstract »  |  Full Text »  |  PDF »  |  Slideshow »

PRESENTATIONS
Endocrinology
The Effect of Acute and Chronic Stress on Growth

Lars Sävendahl (23 October 2012)
Sci. Signal. 5 (247), pt9. [DOI: 10.1126/scisignal.2003484]
 |  Abstract »  |  Full Text »  |  PDF »  |  Slideshow »

PRESENTATIONS
Endocrinology
The Effects of Acute and Chronic Stress on Diabetes Control

M. Loredana Marcovecchio and Francesco Chiarelli (23 October 2012)
Sci. Signal. 5 (247), pt10. [DOI: 10.1126/scisignal.2003508]
 |  Abstract »  |  Full Text »  |  PDF »  |  Slideshow »

PRESENTATIONS
Endocrinology
Neurosteroids and Microneurotrophins Signal Through NGF Receptors to Induce Prosurvival Signaling in Neuronal Cells

Achille Gravanis, Theodora Calogeropoulou, Vily Panoutsakopoulou, Kyriaki Thermos, Constantinos Neophytou, and Ioannis Charalampopoulos (16 October 2012)
Sci. Signal. 5 (246), pt8. [DOI: 10.1126/scisignal.2003387]
 |  Abstract »  |  Full Text »  |  PDF »  |  Slideshow »

PRESENTATIONS
Endocrinology
Posttraumatic Stress Disorder in Children and Adolescents: Neuroendocrine Perspectives

Panagiota Pervanidou and George P. Chrousos (9 October 2012)
Sci. Signal. 5 (245), pt6. [DOI: 10.1126/scisignal.2003327]
 |  Abstract »  |  Full Text »  |  PDF »  |  Slideshow »

PRESENTATIONS
Neuroscience
Fetal Programming of Brain Development: Intrauterine Stress and Susceptibility to Psychopathology

Claudia Buss, Sonja Entringer, and Pathik D. Wadhwa (9 October 2012)
Sci. Signal. 5 (245), pt7. [DOI: 10.1126/scisignal.2003406]
 |  Abstract »  |  Full Text »  |  PDF »  |  Slideshow »

PRESENTATIONS
Endocrinology
Circadian Rhythms of Glucocorticoid Hormone Actions in Target Tissues: Potential Clinical Implications

Tomoshige Kino (2 October 2012)
Sci. Signal. 5 (244), pt4. [DOI: 10.1126/scisignal.2003333]
 |  Abstract »  |  Full Text »  |  PDF »  |  Slideshow »

PRESENTATIONS
Endocrinology
Primary Generalized Glucocorticoid Resistance and Hypersensitivity: The End-Organ Involvement in the Stress Response

Evangelia Charmandari (2 October 2012)
Sci. Signal. 5 (244), pt5. [DOI: 10.1126/scisignal.2003337]
 |  Abstract »  |  Full Text »  |  PDF »  |  Slideshow »