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Sci. STKE, 10 February 2004
Vol. 2004, Issue 219, p. pl5
[DOI: 10.1126/stke.2192004pl5]

PROTOCOLS

Imaging Calcium Concentration Dynamics in Small Neuronal Compartments

Ryohei Yasuda1, Esther A. Nimchinsky1,3, Volker Scheuss1, Thomas A. Pologruto1,2, Thomas G. Oertner1,4, Bernardo L. Sabatini1,5, and Karel Svoboda1*

1Howard Hughes Medical Institute, The Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA.
2Graduate Program in Biophysics, Harvard University, Cambridge, MA 02138, USA.
3Present address: Center for Molecular and Behavioral Neuroscience Aidekman Research Center, Rutgers State University, Newark Campus, 197 University Avenue, Newark, NJ 07102, USA.
4Present address: Friedrich Miescher Institute, WRO-1066.4.10, Maulbeerstrasse 66, CH-4058 Basel, Switzerland.
5Present address: Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.

Abstract: Calcium and its regulation play central roles diverse physiologic processes. Quantification of calcium concentrations ([Ca2+]) in small neuronal compartments is crucial to understanding Ca2+-dependent signaling. Here, we describe techniques that are optimized for 2-photon imaging of [Ca2+] dynamics in small compartments such as dendrites and dendritic spines.

*Corresponding author. Telephone, 516-367-6878; fax, 516-367-8866; e-mail, svoboda{at}cshl.edu

Citation: R. Yasuda, E. A. Nimchinsky, V. Scheuss, T. A. Pologruto, T. G. Oertner, B. L. Sabatini, K. Svoboda, Imaging Calcium Concentration Dynamics in Small Neuronal Compartments. Sci. STKE 2004, pl5 (2004).

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