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Sci. STKE, 6 September 2005
Vol. 2005, Issue 300, p. pe43
[DOI: 10.1126/stke.3002005pe43]

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MicroRNA-Dependent Trans-Acting siRNA Production

Hervé Vaucheret*

Laboratoire de Biologie Cellulaire, Institut Jean-Pierre Bourgin, INRA 78026 Versailles Cedex, France.

Abstract: Less than a year has elapsed between the discovery of trans-acting small interfering RNAs (tasiRNAs) in plants and the elucidation of the major steps of the corresponding pathway. During tasiRNA biogenesis, polyadenylated RNAs transcribed from non–protein-coding TAS genes are cleaved by a microRNA (miRNA)–programmed RNA-induced silencing complex. In contrast to classical miRNA targets, RDR6 and SGS3 convert one of the TAS RNA cleavage products into double-stranded RNA, which is subsequently processed, in a phase determined by the initial miRNA cleavage site, by DICER-LIKE 4 to generate a 21-nucleotide tasiRNA population. tasiRNAs guide endogenous mRNA cleavage through the action of AGO1 or, perhaps in some cases, AGO7. Some of the tasiRNA targets probably regulate the juvenile-to-adult phase transition, but the roles of other tasiRNA targets remain to be determined.

*Contact information. E-mail, herve.vaucheret{at}versailles.inra.fr

Citation: H. Vaucheret, MicroRNA-Dependent Trans-Acting siRNA Production. Sci. STKE 2005, pe43 (2005).

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