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Sci. STKE, 18 April 2006
Vol. 2006, Issue 331, p. re2
Steven S. Vogel*,
Christopher Thaler, and
Srinagesh V. Koushik
National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Laboratory of Molecular Physiology, 5625 Fishers Lane, Bethesda, MD 20892, USA.
The validity of experiments based on Förster resonance energy transfer (FRET), an imaging technique widely used to measure protein-protein interactions in living cells, critically depends on the accurate and precise measurement of FRET efficiency. The use of FRET standards to determine FRET efficiency, and a consideration of such factors as how the abundance of FRET acceptors and the stoichiometry of donors and acceptors in a molecular complex can affect measured FRET efficiency, will enhance the usefulness with which FRET experiments can be interpreted.