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Annu. Rev. Biophys. Biomol. Struct. 29 (1): 49-79

Copyright © 2000 by Annual Reviews.

Annu. Rev. Biophys. Biomol. Struct. 2000. 29:49-79. SIGNALING AND SUBCELLULAR TARGETING BY MEMBRANE-BINDING DOMAINS James H. Hurley and Saurav Misra Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0580; e-mail: jh8e{at}nih.gov C1 domain, C2 domain, FYVE domain, PH domain, subcellular localization

Protein kinase C homology-1 and -2, FYVE, and pleckstrin homology domains are ubiquitous in eukaryotic signal transduction and membrane-trafficking proteins. These domains regulate subcellular localization and protein function by binding to lipid ligands embedded in cell membranes. Structural and biochemical analysis of these domains has shown that their molecular mechanisms of membrane binding depend on a combination of specific and nonspecific interactions with membrane lipids. In vivo studies of green fluorescent protein fusions have highlighted the key roles of these domains in regulating protein localization to plasma and internal membranes in cells.

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