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Genes & Dev. 15 (13): 1716-1723

Copyright © 2001 by Cold Spring Harbor Laboratory Press.

Vol. 15, No. 13, pp. 1716-1723, July 1, 2001

The eyeless homeodomain is dispensable for eye development in Drosophila

Claudio Punzo,1 Shoichiro Kurata,1,2 and Walter J. Gehring1,3

1 Biozentrum, University of Basel, CH-4056 Basel, Switzerland; 2 Graduate School of Pharmaceutical Science, Tohoku University, Aramaki Aoba-ku, Sendai 980-8578, Japan

Pax-6 genes, known to be essential for eye development, encode an evolutionarily conserved transcription factor with two DNA-binding domains. To corroborate the contribution of each DNA-binding domain to eye formation, we generated truncated forms of the Drosophila Pax-6 gene eyeless and tested their capacity to rescue the ey2 mutant. Surprisingly, EY deleted of the homeodomain rescued the ey2 mutant and triggered ectopic eyes morphogenesis. In contrast, EY lacking the paired domain failed to rescue the ey2 mutant, led to truncation of appendages, and repressed Distal-less when misexpressed. This result suggests distinct functions mediated differentially by the two DNA-binding domains of eyeless.

[Key Words: eyeless; Pax-6; eye development; paired domain; homeodomain]

3 Corresponding author.

GENES & DEVELOPMENT 15:1716-1723 © 2001 by Cold Spring Harbor Laboratory Press  ISSN 0890-9369/01 $5.00

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