Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Logo for

Genes & Dev. 15 (18): 2421-2432

Copyright © 2001 by Cold Spring Harbor Laboratory Press.

Vol. 15, No. 18, pp. 2421-2432, September 15, 2001

RESEARCH PAPER
Requirement of the JIP1 scaffold protein for stress-induced JNK activation

Alan J. Whitmarsh,1,6,8 Chia-Yi Kuan,4,7,8 Norman J. Kennedy,1,8 Nyaya Kelkar,1,8 Tarik F. Haydar,4 John P. Mordes,2 Michael Appel,2 Aldo A. Rossini,2 Stephen N. Jones,3 Richard A. Flavell,5 Pasko Rakic,4 and Roger J. Davis1,9

1 Howard Hughes Medical Institute and Program in Molecular Medicine, 2 Program in Molecular Medicine and Division of Diabetes, Department of Medicine, and 3 Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA; 4 Section of Neurobiology and 5 Howard Hughes Medical Institute and Section of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA

The c-Jun N-terminal kinase (JNK) signal transduction pathway is activated in response to the exposure of cells to environmental stress. Components of the JNK signaling pathway interact with the JIP1 scaffold protein. JIP1 is located in the neurites of primary hippocampal neurons. However, in response to stress, JIP1 accumulates in the soma together with activated JNK and phosphorylated c-Jun. Disruption of the Jip1 gene in mice by homologous recombination prevented JNK activation caused by exposure to excitotoxic stress and anoxic stress in vivo and in vitro. These data show that the JIP1 scaffold protein is a critical component of a MAP-kinase signal transduction pathway.

[Key Words: MAP kinase; scaffold; JIP1; JNK]


Present addresses: 6School of Biological Sciences, University of Manchester, Manchester, UK; 7Division of Developmental Biology, Cincinnati Children's Hospital Research Foundation, Cincinnati, OH 45229, USA.

8 These authors contributed equally to this work.

9 Corresponding author.


GENES & DEVELOPMENT 15:2421-2432 © 2001 by Cold Spring Harbor Laboratory Press  ISSN 0890-9369/01 $5.00

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Regulation of axon growth by the JIP1-AKT axis.
F. Dajas-Bailador, I. Bantounas, E. V. Jones, and A. J. Whitmarsh (2014)
J. Cell Sci. 127, 230-239
   Abstract »    Full Text »    PDF »
The Small GTPase RALA Controls c-Jun N-terminal Kinase-mediated FOXO Activation by Regulation of a JIP1 Scaffold Complex.
M. C. W. van den Berg, I. J. A. van Gogh, A. M. M. Smits, M. van Triest, T. B. Dansen, M. Visscher, P. E. Polderman, M. J. Vliem, H. Rehmann, and B. M. T. Burgering (2013)
J. Biol. Chem. 288, 21729-21741
   Abstract »    Full Text »    PDF »
Mammalian MAPK Signal Transduction Pathways Activated by Stress and Inflammation: A 10-Year Update.
J. M. Kyriakis and J. Avruch (2012)
Physiol Rev 92, 689-737
   Abstract »    Full Text »    PDF »
Sh3rf2/POSHER Protein Promotes Cell Survival by Ring-mediated Proteasomal Degradation of the c-Jun N-terminal Kinase Scaffold POSH (Plenty of SH3s) Protein.
M. Wilhelm, N. V. Kukekov, T. L. Schmit, K. V. Biagas, A. A. Sproul, S. Gire, M. E. Maes, Z. Xu, and L. A. Greene (2012)
J. Biol. Chem. 287, 2247-2256
   Abstract »    Full Text »    PDF »
Requirement of JIP1-Mediated c-Jun N-Terminal Kinase Activation for Obesity-Induced Insulin Resistance.
C. Morel, C. L. Standen, D. Y. Jung, S. Gray, H. Ong, R. A. Flavell, J. K. Kim, and R. J. Davis (2010)
Mol. Cell. Biol. 30, 4616-4625
   Abstract »    Full Text »    PDF »
Distinct Roles of c-Jun N-Terminal Kinase Isoforms in Neurite Initiation and Elongation during Axonal Regeneration.
M. Barnat, H. Enslen, F. Propst, R. J. Davis, S. Soares, and F. Nothias (2010)
J. Neurosci. 30, 7804-7816
   Abstract »    Full Text »    PDF »
Signal Transduction Cross Talk Mediated by Jun N-Terminal Kinase-Interacting Protein and Insulin Receptor Substrate Scaffold Protein Complexes.
C. L. Standen, N. J. Kennedy, R. A. Flavell, and R. J. Davis (2009)
Mol. Cell. Biol. 29, 4831-4840
   Abstract »    Full Text »    PDF »
Vaccinia-Related Kinase 2 Modulates the Stress Response to Hypoxia Mediated by TAK1.
S. Blanco, C. Santos, and P. A. Lazo (2007)
Mol. Cell. Biol. 27, 7273-7283
   Abstract »    Full Text »    PDF »
Requirement of JIP scaffold proteins for NMDA-mediated signal transduction.
N. J. Kennedy, G. Martin, A. G. Ehrhardt, J. Cavanagh-Kyros, C.-Y. Kuan, P. Rakic, R. A. Flavell, S. N. Treistman, and R. J. Davis (2007)
Genes & Dev. 21, 2336-2346
   Abstract »    Full Text »    PDF »
T-Lymphokine-Activated Killer Cell-Originated Protein Kinase Functions as a Positive Regulator of c-Jun-NH2-Kinase 1 Signaling and H-Ras-Induced Cell Transformation.
S.-M. Oh, F. Zhu, Y.-Y. Cho, K. W. Lee, B. S. Kang, H.-G. Kim, T. Zykova, A. M. Bode, and Z. Dong (2007)
Cancer Res. 67, 5186-5194
   Abstract »    Full Text »    PDF »
Src Family Kinases Directly Regulate JIP1 Module Dynamics and Activation.
D. Nihalani, H. Wong, R. Verma, and L. B. Holzman (2007)
Mol. Cell. Biol. 27, 2431-2441
   Abstract »    Full Text »    PDF »
PKC-{alpha} and TAK-1 are intermediates in the activation of c-Jun NH2-terminal kinase by hypoxia-reoxygenation.
D. P. Frazier, A. Wilson, C. J. Dougherty, H. Li, N. H. Bishopric, and K. A. Webster (2007)
Am J Physiol Heart Circ Physiol 292, H1675-H1684
   Abstract »    Full Text »    PDF »
Peptides Targeting Protein Kinases: Strategies and Implications..
O. Kaidanovich-Beilin and H. Eldar-Finkelman (2006)
Physiology 21, 411-418
   Abstract »    Full Text »    PDF »
Vaccinia Virus B1R Kinase Interacts with JIP1 and Modulates c-Jun-Dependent Signaling.
C. R. Santos, S. Blanco, A. Sevilla, and P. A. Lazo (2006)
J. Virol. 80, 7667-7675
   Abstract »    Full Text »    PDF »
Direct Interaction of the Molecular Scaffolds POSH and JIP Is Required for Apoptotic Activation of JNKs.
N. V. Kukekov, Z. Xu, and L. A. Greene (2006)
J. Biol. Chem. 281, 15517-15524
   Abstract »    Full Text »    PDF »
JNK1 phosphorylation of SCG10 determines microtubule dynamics and axodendritic length.
T. Tararuk, N. Ostman, W. Li, B. Bjorkblom, A. Padzik, J. Zdrojewska, V. Hongisto, T. Herdegen, W. Konopka, M. J. Courtney, et al. (2006)
J. Cell Biol. 173, 265-277
   Abstract »    Full Text »    PDF »
A unique set of SH3-SH3 interactions controls IB1 homodimerization.
O. Kristensen, S. Guenat, I. Dar, N. Allaman-Pillet, A. Abderrahmani, M. Ferdaoussi, R. Roduit, F. Maurer, J. S. Beckmann, J. S. Kastrup, et al. (2006)
EMBO J. 25, 785-797
   Abstract »    Full Text »    PDF »
Coordinated transport of phosphorylated amyloid-{beta} precursor protein and c-Jun NH2-terminal kinase-interacting protein-1.
Z. Muresan and V. Muresan (2005)
J. Cell Biol. 171, 615-625
   Abstract »    Full Text »    PDF »
Regulation of Apoptotic c-Jun N-Terminal Kinase Signaling by a Stabilization-Based Feed-Forward Loop.
Z. Xu, N. V. Kukekov, and L. A. Greene (2005)
Mol. Cell. Biol. 25, 9949-9959
   Abstract »    Full Text »    PDF »
JSAP1/JIP3 Cooperates with Focal Adhesion Kinase to Regulate c-Jun N-terminal Kinase and Cell Migration.
T. Takino, M. Nakada, H. Miyamori, Y. Watanabe, T. Sato, D. Gantulga, K. Yoshioka, K. M. Yamada, and H. Sato (2005)
J. Biol. Chem. 280, 37772-37781
   Abstract »    Full Text »    PDF »
Notch interferes with the scaffold function of JNK-interacting protein 1 to inhibit the JNK signaling pathway.
J. W. Kim, M. J. Kim, K. J. Kim, H. J. Yun, J. S. Chae, S. G. Hwang, T.-S. Chang, H.-S. Park, K.-W. Lee, P.-L. Han, et al. (2005)
PNAS 102, 14308-14313
   Abstract »    Full Text »    PDF »
The Molecular Scaffold Kinase Suppressor of Ras 1 (KSR1) Regulates Adipogenesis.
R. L. Kortum, D. L. Costanzo, J. Haferbier, S. J. Schreiner, G. L. Razidlo, M.-H. Wu, D. J. Volle, T. Mori, H. Sakaue, N. V. Chaika, et al. (2005)
Mol. Cell. Biol. 25, 7592-7604
   Abstract »    Full Text »    PDF »
Cross-talk between JIP3 and JIP1 during Glucose Deprivation: SEK1-JNK2 AND Akt1 ACT AS MEDIATORS.
J. J. Song and Y. J. Lee (2005)
J. Biol. Chem. 280, 26845-26855
   Abstract »    Full Text »    PDF »
Dissociation of Akt1 from its negative regulator JIP1 is mediated through the ASK1-MEK-JNK signal transduction pathway during metabolic oxidative stress: a negative feedback loop.
J. J. Song and Y. J. Lee (2005)
J. Cell Biol. 170, 61-72
   Abstract »    Full Text »    PDF »
Fructose Selectively Modulates c-jun N-Terminal Kinase Activity and Insulin Signaling in Rat Primary Hepatocytes.
Y. Wei, D. Wang, and M. J. Pagliassotti (2005)
J. Nutr. 135, 1642-1646
   Abstract »    Full Text »    PDF »
c-Jun NH2-Terminal Kinase-Interacting Protein-3 Facilitates Phosphorylation and Controls Localization of Amyloid-{beta} Precursor Protein.
Z. Muresan and V. Muresan (2005)
J. Neurosci. 25, 3741-3751
   Abstract »    Full Text »    PDF »
Role of the JIP4 Scaffold Protein in the Regulation of Mitogen-Activated Protein Kinase Signaling Pathways.
N. Kelkar, C. L. Standen, and R. J. Davis (2005)
Mol. Cell. Biol. 25, 2733-2743
   Abstract »    Full Text »    PDF »
Sunday Driver links axonal transport to damage signaling.
V. Cavalli, P. Kujala, J. Klumperman, and L. S.B. Goldstein (2005)
J. Cell Biol. 168, 775-787
   Abstract »    Full Text »    PDF »
The ALX Src Homology 2 Domain Is Both Necessary and Sufficient to Inhibit T Cell receptor/CD28-mediated Up-regulation of RE/AP.
M. J. Shapiro, P. Powell, A. Ndubuizu, C. Nzerem, and V. S. Shapiro (2004)
J. Biol. Chem. 279, 40647-40652
   Abstract »    Full Text »    PDF »
An essential role of the JIP1 scaffold protein for JNK activation in adipose tissue.
A. Jaeschke, M. P. Czech, and R. J. Davis (2004)
Genes & Dev. 18, 1976-1980
   Abstract »    Full Text »    PDF »
Mitochondrial signals initiate the activation of c-Jun N-terminal kinase (JNK) by hypoxia-reoxygenation.
C. J. DOUGHERTY, L. A. KUBASIAK, D. P. FRAZIER, H. LI, W.-CHENG. XIONG, N. H. BISHOPRIC, and K. A. WEBSTER (2004)
FASEB J 18, 1060-1070
   Abstract »    Full Text »    PDF »
Scaffold Proteins in Mammalian MAP Kinase Cascades.
K. Yoshioka (2004)
J. Biochem. 135, 657-661
   Abstract »    Full Text »    PDF »
Modular construction of a signaling scaffold: MORG1 interacts with components of the ERK cascade and links ERK signaling to specific agonists.
T. Vomastek, H.-J. Schaeffer, A. Tarcsafalvi, M. E. Smolkin, E. A. Bissonette, and M. J. Weber (2004)
PNAS 101, 6981-6986
   Abstract »    Full Text »    PDF »
Complexin I regulates glucose-induced secretion in pancreatic {beta}-cells.
A. Abderrahmani, G. Niederhauser, V. Plaisance, M.-E. Roehrich, V. Lenain, T. Coppola, R. Regazzi, and G. Waeber (2004)
J. Cell Sci. 117, 2239-2247
   Abstract »    Full Text »    PDF »
Docking Interactions in the c-Jun N-terminal Kinase Pathway.
L. M. Mooney and A. J. Whitmarsh (2004)
J. Biol. Chem. 279, 11843-11852
   Abstract »    Full Text »    PDF »
A critical role of neural-specific JNK3 for ischemic apoptosis.
C.-Y. Kuan, A. J. Whitmarsh, D. D. Yang, G. Liao, A. J. Schloemer, C. Dong, J. Bao, K. J. Banasiak, G. G. Haddad, R. A. Flavell, et al. (2003)
PNAS 100, 15184-15189
   Abstract »    Full Text »    PDF »
The Transcriptional Repressor HBP1 Is a Target of the p38 Mitogen-Activated Protein Kinase Pathway in Cell Cycle Regulation.
M. Xiu, J. Kim, E. Sampson, C.-Y. Huang, R. J. Davis, K. E. Paulson, and A. S. Yee (2003)
Mol. Cell. Biol. 23, 8890-8901
   Abstract »    Full Text »    PDF »
Inflammatory Cytokine Expression Is Independent of the c-Jun N-Terminal Kinase/AP-1 Signaling Cascade in Human Neutrophils.
A. Cloutier, T. Ear, O. Borissevitch, P. Larivee, and P. P. McDonald (2003)
J. Immunol. 171, 3751-3761
   Abstract »    Full Text »    PDF »
Lithium Blocks the c-Jun Stress Response and Protects Neurons via Its Action on Glycogen Synthase Kinase 3.
V. Hongisto, N. Smeds, S. Brecht, T. Herdegen, M. J. Courtney, and E. T. Coffey (2003)
Mol. Cell. Biol. 23, 6027-6036
   Abstract »    Full Text »    PDF »
JNK-interacting protein 3 associates with Toll-like receptor 4 and is involved in LPS-mediated JNK activation.
T. Matsuguchi, A. Masuda, K. Sugimoto, Y. Nagai, and Y. Yoshikai (2003)
EMBO J. 22, 4455-4464
   Abstract »    Full Text »    PDF »
Morphogenesis of the telencephalic commissure requires scaffold protein JNK-interacting protein 3 (JIP3).
N. Kelkar, M.-H. Delmotte, C. R. Weston, T. Barrett, B. J. Sheppard, R. A. Flavell, and R. J. Davis (2003)
PNAS 100, 9843-9848
   Abstract »    Full Text »    PDF »
JNK-interacting Protein 1 Promotes Akt1 Activation.
A. H. Kim, T. Sasaki, and M. V. Chao (2003)
J. Biol. Chem. 278, 29830-29836
   Abstract »    Full Text »    PDF »
Recruitment of JNK to JIP1 and JNK-dependent JIP1 Phosphorylation Regulates JNK Module Dynamics and Activation.
D. Nihalani, H. N. Wong, and L. B. Holzman (2003)
J. Biol. Chem. 278, 28694-28702
   Abstract »    Full Text »    PDF »
Enhanced activation of axonally transported stress-activated protein kinases in peripheral nerve in diabetic neuropathy is prevented by neurotrophin-3.
A. Middlemas, J.-D. Delcroix, N. M. Sayers, D. R. Tomlinson, and P. Fernyhough (2003)
Brain 126, 1671-1682
   Abstract »    Full Text »    PDF »
A Scaffold Protein JIP-1b Enhances Amyloid Precursor Protein Phosphorylation by JNK and Its Association with Kinesin Light Chain 1.
H. Inomata, Y. Nakamura, A. Hayakawa, H. Takata, T. Suzuki, K. Miyazawa, and N. Kitamura (2003)
J. Biol. Chem. 278, 22946-22955
   Abstract »    Full Text »    PDF »
The JNK-interacting Protein-1 Scaffold Protein Targets MAPK Phosphatase-7 to Dephosphorylate JNK.
E. A. Willoughby, G. R. Perkins, M. K. Collins, and A. J. Whitmarsh (2003)
J. Biol. Chem. 278, 10731-10736
   Abstract »    Full Text »    PDF »
POSH acts as a scaffold for a multiprotein complex that mediates JNK activation in apoptosis.
Z. Xu, N. V. Kukekov, and L. A. Greene (2003)
EMBO J. 22, 252-261
   Abstract »    Full Text »    PDF »
Fibroblast Growth Factor Homologous Factors and the Islet Brain-2 Scaffold Protein Regulate Activation of a Stress-activated Protein Kinase.
J. Schoorlemmer and M. Goldfarb (2002)
J. Biol. Chem. 277, 49111-49119
   Abstract »    Full Text »    PDF »
The p65/RelA Subunit of NF-{kappa}B Suppresses the Sustained, Antiapoptotic Activity of Jun Kinase Induced by Tumor Necrosis Factor.
J. Y. Reuther-Madrid, D. Kashatus, S. Chen, X. Li, J. Westwick, R. J. Davis, H. S. Earp, C.-Y. Wang, and A. S. Baldwin Jr. (2002)
Mol. Cell. Biol. 22, 8175-8183
   Abstract »    Full Text »    PDF »
Evidence of Functional Modulation of the MEKK/JNK/cJun Signaling Cascade by the Low Density Lipoprotein Receptor-related Protein (LRP).
C. Lutz, J. Nimpf, M. Jenny, K. Boecklinger, C. Enzinger, G. Utermann, G. Baier-Bitterlich, and G. Baier (2002)
J. Biol. Chem. 277, 43143-43151
   Abstract »    Full Text »    PDF »
JLP: A scaffolding protein that tethers JNK/p38MAPK signaling modules and transcription factors.
C. M. Lee, D. Onesime, C. D. Reddy, N. Dhanasekaran, and E. P. Reddy (2002)
PNAS 99, 14189-14194
   Abstract »    Full Text »    PDF »
Phosphorylation-dependent Scaffolding Role of JSAP1/JIP3 in the ASK1-JNK Signaling Pathway: A NEW MODE OF REGULATION OF THE MAP KINASE CASCADE.
H. Matsuura, H. Nishitoh, K. Takeda, A. Matsuzawa, T. Amagasa, M. Ito, K. Yoshioka, and H. Ichijo (2002)
J. Biol. Chem. 277, 40703-40709
   Abstract »    Full Text »    PDF »
The Activation of Rac1 by M3 Muscarinic Acetylcholine Receptors Involves the Translocation of Rac1 and IQGAP1 to Cell Junctions and Changes in the Composition of Protein Complexes Containing Rac1, IQGAP1, and Actin.
R. Ruiz-Velasco, C. C. Lanning, and C. L. Williams (2002)
J. Biol. Chem. 277, 33081-33091
   Abstract »    Full Text »    PDF »
Interaction of Rac Exchange Factors Tiam1 and Ras-GRF1 with a Scaffold for the p38 Mitogen-Activated Protein Kinase Cascade.
R. J. Buchsbaum, B. A. Connolly, and L. A. Feig (2002)
Mol. Cell. Biol. 22, 4073-4085
   Abstract »    Full Text »    PDF »
Kinase Suppressor of Ras (KSR) Is a Scaffold Which Facilitates Mitogen-Activated Protein Kinase Activation In Vivo.
A. Nguyen, W. R. Burack, J. L. Stock, R. Kortum, O. V. Chaika, M. Afkarian, W. J. Muller, K. M. Murphy, D. K. Morrison, R. E. Lewis, et al. (2002)
Mol. Cell. Biol. 22, 3035-3045
   Abstract »    Full Text »    PDF »
Jun NH2-terminal Kinase (JNK) Interacting Protein 1 (JIP1) Binds the Cytoplasmic Domain of the Alzheimer's beta -Amyloid Precursor Protein (APP).
M. H. Scheinfeld, R. Roncarati, P. Vito, P. A. Lopez, M. Abdallah, and L. D'Adamio (2002)
J. Biol. Chem. 277, 3767-3775
   Abstract »    Full Text »    PDF »

To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882