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RESEARCH PAPER
PIASy, a nuclear matrix-associated SUMO E3 ligase, represses LEF1 activity by sequestration into nuclear bodies
Shrikesh
Sachdev,1
Laurakay
Bruhn,1
Heidemarie
Sieber,1
Andrea
Pichler,2
Frauke
Melchior,2 and
Rudolf
Grosschedl1,3
1 Gene Center and Institute of Biochemistry, University of
Munich, 81377 Munich, Germany; and 2 Max-Planck Institute
of Biochemistry, 82152 Martinsried, Germany
The Wnt-responsive transcription factor LEF1 can activate
transcription in association with -catenin and repress transcriptionin association with Groucho. In search of additional regulatorymechanisms of LEF1 function, we identified the protein inhibitorof
activated STAT, PIASy, as a novel interaction partner of LEF1.Coexpression of PIASy with LEF1 results in potent repression ofLEF1
activity and in covalent modification of LEF1 with SUMO.PIASy markedly
stimulates the sumoylation of LEF1 and multipleother proteins in vivo
and functions as a SUMO E3 ligase for LEF1in a reconstituted system in
vitro. Moreover, PIASy binds to nuclearmatrix-associated DNA
sequences and targets LEF1 to nuclear bodies,suggesting that PIASy-mediated
subnuclear sequestration accountsfor the repression of LEF1activity.
Protein Inhibitors of Activated STAT (Pias1 and Piasy) Differentially Regulate Pituitary Homeobox 2 (PITX2) Transcriptional Activity.
J. Wang, Z. Sun, Z. Zhang, I. Saadi, J. Wang, X. Li, S. Gao, J. J. Engle, A. Kuburas, X. Fu, et al. (2013)
J. Biol. Chem.
288, 12580-12595
|Abstract »|Full Text »|PDF »
UHRF2, a Ubiquitin E3 Ligase, Acts as a Small Ubiquitin-like Modifier E3 Ligase for Zinc Finger Protein 131.
The in Vivo Role of Androgen Receptor SUMOylation as Revealed by Androgen Insensitivity Syndrome and Prostate Cancer Mutations Targeting the Proline/Glycine Residues of Synergy Control Motifs.
S. Mukherjee, O. Cruz-Rodriguez, E. Bolton, and J. A. Iniguez-Lluhi (2012)
J. Biol. Chem.
287, 31195-31206
|Abstract »|Full Text »|PDF »
CBX4-mediated SUMO modification regulates BMI1 recruitment at sites of DNA damage.
I. H. Ismail, J.-P. Gagne, M.-C. Caron, D. McDonald, Z. Xu, J.-Y. Masson, G. G. Poirier, and M. J. Hendzel (2012)
Nucleic Acids Res.
40, 5497-5510
|Abstract »|Full Text »|PDF »
Characterization of a PIAS4 Homologue from Zebrafish: Insights into Its Conserved Negative Regulatory Mechanism in the TRIF, MAVS, and IFN Signaling Pathways during Vertebrate Evolution.
R. Xiong, L. Nie, L.-x. Xiang, and J.-z. Shao (2012)
J. Immunol.
188, 2653-2668
|Abstract »|Full Text »|PDF »
UBC9 Autosumoylation Negatively Regulates Sumoylation of Septins in Saccharomyces cerevisiae.
PKC{zeta} mediates disturbed flow-induced endothelial apoptosis via p53 SUMOylation.
K.-S. Heo, H. Lee, P. Nigro, T. Thomas, N.-T. Le, E. Chang, C. McClain, C. A. Reinhart-King, M. R. King, B. C. Berk, et al. (2011)
J. Cell Biol.
193, 867-884
|Abstract »|Full Text »|PDF »
PIASy Inhibits Virus-induced and Interferon-stimulated Transcription through Distinct Mechanisms.
T. Kubota, M. Matsuoka, S. Xu, N. Otsuki, M. Takeda, A. Kato, and K. Ozato (2011)
J. Biol. Chem.
286, 8165-8175
|Abstract »|Full Text »|PDF »
Molecular Determinants for Small Maf Protein Control of Platelet Production.
H. Motohashi, R. Fujita, M. Takayama, A. Inoue, F. Katsuoka, E. H. Bresnick, and M. Yamamoto (2011)
Mol. Cell. Biol.
31, 151-162
|Abstract »|Full Text »|PDF »
PIAS1 regulates CP2c localization and active promoter complex formation in erythroid cell-specific {alpha}-globin expression.
H. Chul Kang, J. Hyung Chae, J. Jeon, W. Kim, D. Hyun Ha, J. Ho Shin, C. Gil Kim, and C. Geun Kim (2010)
Nucleic Acids Res.
38, 5456-5471
|Abstract »|Full Text »|PDF »
Phosphorylation-Dependent Interaction of SATB1 and PIAS1 Directs SUMO-Regulated Caspase Cleavage of SATB1.
J. A. T. Tan, J. Song, Y. Chen, and L. K. Durrin (2010)
Mol. Cell. Biol.
30, 2823-2836
|Abstract »|Full Text »|PDF »
The SUMO E3 Ligase Activity of Pc2 Is Coordinated through a SUMO Interaction Motif.
A Novel Role for Protein Inhibitor of Activated STAT (PIAS) Proteins in Modulating the Activity of Zimp7, a Novel PIAS-like Protein, in Androgen Receptor-mediated Transcription.
Y. Peng, J. Lee, C. Zhu, and Z. Sun (2010)
J. Biol. Chem.
285, 11465-11475
|Abstract »|Full Text »|PDF »
Specific Domain Structures Control Abscisic Acid-, Salicylic Acid-, and Stress-Mediated SIZ1 Phenotypes.
M. S. Cheong, H. C. Park, M. J. Hong, J. Lee, W. Choi, J. B. Jin, H. J. Bohnert, S. Y. Lee, R. A. Bressan, and D.-J. Yun (2009)
Plant Physiology
151, 1930-1942
|Abstract »|Full Text »|PDF »
SUMOylation of the mitochondrial fission protein Drp1 occurs at multiple nonconsensus sites within the B domain and is linked to its activity cycle.
C. Figueroa-Romero, J. A. Iniguez-Lluhi, J. Stadler, C.-R. Chang, D. Arnoult, P. J. Keller, Y. Hong, C. Blackstone, and E. L. Feldman (2009)
FASEB J
23, 3917-3927
|Abstract »|Full Text »|PDF »
Small Ubiquitin-like Modifier (SUMO) Modification of the Androgen Receptor Attenuates Polyglutamine-mediated Aggregation.
S. Mukherjee, M. Thomas, N. Dadgar, A. P. Lieberman, and J. A. Iniguez-Lluhi (2009)
J. Biol. Chem.
284, 21296-21306
|Abstract »|Full Text »|PDF »
Dazap2 modulates transcription driven by the Wnt effector TCF-4.
J. Lukas, P. Mazna, T. Valenta, L. Doubravska, V. Pospichalova, M. Vojtechova, B. Fafilek, R. Ivanek, J. Plachy, J. Novak, et al. (2009)
Nucleic Acids Res.
37, 3007-3020
|Abstract »|Full Text »|PDF »
Sumoylation of the Transcription Factor NFATc1 Leads to Its Subnuclear Relocalization and Interleukin-2 Repression by Histone Deacetylase.
A. Nayak, J. Glockner-Pagel, M. Vaeth, J. E. Schumann, M. Buttmann, T. Bopp, E. Schmitt, E. Serfling, and F. Berberich-Siebelt (2009)
J. Biol. Chem.
284, 10935-10946
|Abstract »|Full Text »|PDF »
PIAS3 negatively regulates RANKL-mediated osteoclastogenesis directly in osteoclast precursors and indirectly via osteoblasts.
T. Hikata, H. Takaishi, J. Takito, A. Hakozaki, M. Furukawa, S. Uchikawa, T. Kimura, Y. Okada, M. Matsumoto, A. Yoshimura, et al. (2009)
Blood
113, 2202-2212
|Abstract »|Full Text »|PDF »
SUMOylation Inhibits SF-1 Activity by Reducing CDK7-Mediated Serine 203 Phosphorylation.
W.-H. Yang, J. H. Heaton, H. Brevig, S. Mukherjee, J. A. Iniguez-Lluhi, and G. D. Hammer (2009)
Mol. Cell. Biol.
29, 613-625
|Abstract »|Full Text »|PDF »
Molecular Basis for SUMOylation-dependent Regulation of DNA Binding Activity of Heat Shock Factor 2.
Y. Tateishi, M. Ariyoshi, R. Igarashi, H. Hara, K. Mizuguchi, A. Seto, A. Nakai, T. Kokubo, H. Tochio, and M. Shirakawa (2009)
J. Biol. Chem.
284, 2435-2447
|Abstract »|Full Text »|PDF »
SUMO-Mediated Inhibition of Glucocorticoid Receptor Synergistic Activity Depends on Stable Assembly at the Promoter But Not on DAXX.
S. R. Holmstrom, S. Chupreta, A. Y.-L. So, and J. A. Iniguez-Lluhi (2008)
Mol. Endocrinol.
22, 2061-2075
|Abstract »|Full Text »|PDF »
The PHD Domain of Plant PIAS Proteins Mediates Sumoylation of Bromodomain GTE Proteins.
M. Garcia-Dominguez, R. March-Diaz, and J. C. Reyes (2008)
J. Biol. Chem.
283, 21469-21477
|Abstract »|Full Text »|PDF »
PIASy Represses CCAAT/Enhancer-binding Protein {delta} (C/EBP{delta}) Transcriptional Activity by Sequestering C/EBP{delta} to the Nuclear Periphery.
S. Zhou, J. Si, T. Liu, and J. W. DeWille (2008)
J. Biol. Chem.
283, 20137-20148
|Abstract »|Full Text »|PDF »
SUMO Assay with Peptide Arrays on Solid Support: Insights into SUMO Target Sites.
K. Schwamborn, P. Knipscheer, E. van Dijk, W. J. van Dijk, T. K. Sixma, R. H. Meloen, and J. P.M. Langedijk (2008)
J. Biochem.
144, 39-49
|Abstract »|Full Text »|PDF »
Signal transducer and activator of transcription 5A/B in prostate and breast cancers.
Spatial Interplay between PIASy and FIP200 in the Regulation of Signal Transduction and Transcriptional Activity.
N. Martin, K. Schwamborn, H. Urlaub, B. Gan, J.-L. Guan, and A. Dejean (2008)
Mol. Cell. Biol.
28, 2771-2781
|Abstract »|Full Text »|PDF »
SUMOylation of HMGA2: selective destabilization of promyelocytic leukemia protein via proteasome.
X. Cao, C. Clavijo, X. Li, H. H. Lin, Y. Chen, H.-M. Shih, and D. K. Ann (2008)
Mol. Cancer Ther.
7, 923-934
|Abstract »|Full Text »|PDF »
Transforming Growth Factor-{beta}1 Attenuates Expression of Both the Progesterone Receptor and Dickkopf in Differentiated Human Endometrial Stromal Cells.
N. Kane, M. Jones, J. J. Brosens, P. T. K. Saunders, R. W. Kelly, and H. O. D. Critchley (2008)
Mol. Endocrinol.
22, 716-728
|Abstract »|Full Text »|PDF »
In Vivo Identification of Human Small Ubiquitin-like Modifier Polymerization Sites by High Accuracy Mass Spectrometry and an in Vitro to in Vivo Strategy.
I. Matic, M. van Hagen, J. Schimmel, B. Macek, S. C. Ogg, M. H. Tatham, R. T. Hay, A. I. Lamond, M. Mann, and A. C. O. Vertegaal (2008)
Mol. Cell. Proteomics
7, 132-144
|Abstract »|Full Text »|PDF »
Sumoylation-dependent Control of Homotypic and Heterotypic Synergy by the Kruppel-type Zinc Finger Protein ZBP-89.
S. Chupreta, H. Brevig, L. Bai, J. L. Merchant, and J. A. Iniguez-Lluhi (2007)
J. Biol. Chem.
282, 36155-36166
|Abstract »|Full Text »|PDF »
MUC1 Expression Is Repressed by Protein Inhibitor of Activated Signal Transducer and Activator of Transcription-y.
M. J. Brayman, N. Dharmaraj, E. Lagow, and D. D. Carson (2007)
Mol. Endocrinol.
21, 2725-2737
|Abstract »|Full Text »|PDF »
Topoisomerase I-Dependent Viability Loss in Saccharomyces cerevisiae Mutants Defective in Both SUMO Conjugation and DNA Repair.
X. L. Chen, H. R. Silver, L. Xiong, I. Belichenko, C. Adegite, and E. S. Johnson (2007)
Genetics
177, 17-30
|Abstract »|Full Text »|PDF »
The Arabidopsis E3 SUMO Ligase SIZ1 Regulates Plant Growth and Drought Responses.
R. Catala, J. Ouyang, I. A. Abreu, Y. Hu, H. Seo, X. Zhang, and N.-H. Chua (2007)
PLANT CELL
19, 2952-2966
|Abstract »|Full Text »|PDF »
PIASxbeta is a key regulator of osterix transcriptional activity and matrix mineralization in osteoblasts.
Md. M. Ali, T. Yoshizawa, O. Ishibashi, A. Matsuda, M. Ikegame, J. Shimomura, H. Mera, K. Nakashima, and H. Kawashima (2007)
J. Cell Sci.
120, 2565-2573
|Abstract »|Full Text »|PDF »
Control of specificity and magnitude of NF-{kappa}B and STAT1-mediated gene activation through PIASy and PIAS1 cooperation.
S. Tahk, B. Liu, V. Chernishof, K. A. Wong, H. Wu, and K. Shuai (2007)
PNAS
104, 11643-11648
|Abstract »|Full Text »|PDF »
PIASy-Mediated Sumoylation of Yin Yang 1 Depends on Their Interaction but Not the RING Finger.
Sumoylation of the Transcriptional Intermediary Factor 1beta (TIF1beta), the Co-repressor of the KRAB Multifinger Proteins, Is Required for Its Transcriptional Activity and Is Modulated by the KRAB Domain.
X. H. Mascle, D. Germain-Desprez, P. Huynh, P. Estephan, and M. Aubry (2007)
J. Biol. Chem.
282, 10190-10202
|Abstract »|Full Text »|PDF »
SIZ1-Mediated Sumoylation of ICE1 Controls CBF3/DREB1A Expression and Freezing Tolerance in Arabidopsis.
K. Miura, J. B. Jin, J. Lee, C. Y. Yoo, V. Stirm, T. Miura, E. N. Ashworth, R. A. Bressan, D.-J. Yun, and P. M. Hasegawa (2007)
PLANT CELL
19, 1403-1414
|Abstract »|Full Text »|PDF »
Stress-induced Inactivation of the c-Myb Transcription Factor through Conjugation of SUMO-2/3 Proteins.
M. Sramko, J. Markus, J. Kabat, L. Wolff, and J. Bies (2006)
J. Biol. Chem.
281, 40065-40075
|Abstract »|Full Text »|PDF »
Distinct and Overlapping Sets of SUMO-1 and SUMO-2 Target Proteins Revealed by Quantitative Proteomics.
A. C. O. Vertegaal, J. S. Andersen, S. C. Ogg, R. T. Hay, M. Mann, and A. I. Lamond (2006)
Mol. Cell. Proteomics
5, 2298-2310
|Abstract »|Full Text »|PDF »
Ubc9 Regulates Mitosis and Cell Survival during Zebrafish Development.
Multiple domains in Siz SUMO ligases contribute to substrate selectivity.
A. Reindle, I. Belichenko, G. R. Bylebyl, X. L. Chen, N. Gandhi, and E. S. Johnson (2006)
J. Cell Sci.
119, 4749-4757
|Abstract »|Full Text »|PDF »
Stat1 and SUMO modification.
L. Song, S. Bhattacharya, A. A. Yunus, C. D. Lima, and C. Schindler (2006)
Blood
108, 3237-3244
|Abstract »|Full Text »|PDF »
PIAS3 induction of PRB sumoylation represses PRB transactivation by destabilizing its retention in the nucleus.
J.-H. Man, H.-Y. Li, P.-J. Zhang, T. Zhou, K. He, X. Pan, B. Liang, A.-L. Li, J. Zhao, W.-L. Gong, et al. (2006)
Nucleic Acids Res.
34, 5552-5566
|Abstract »|Full Text »|PDF »
Functional Interaction between Human Herpesvirus 6 Immediate-Early 2 Protein and Ubiquitin-Conjugating Enzyme 9 in the Absence of Sumoylation.
A. Tomoiu, A. Gravel, R. M. Tanguay, and L. Flamand (2006)
J. Virol.
80, 10218-10228
|Abstract »|Full Text »|PDF »
Negative Modulation of RXR{alpha} Transcriptional Activity by Small Ubiquitin-related Modifier (SUMO) Modification and Its Reversal by SUMO-specific Protease SUSP1.
S. J. Choi, S. S. Chung, E. J. Rho, H. W. Lee, M. H. Lee, H.-S. Choi, J. H. Seol, S. H. Baek, O. S. Bang, and C. H. Chung (2006)
J. Biol. Chem.
281, 30669-30677
|Abstract »|Full Text »|PDF »
Sumoylation of CCAAT/Enhancer-binding Protein {alpha} and Its Functional Roles in Hepatocyte Differentiation.
Y. Sato, K. Miyake, H. Kaneoka, and S. Iijima (2006)
J. Biol. Chem.
281, 21629-21639
|Abstract »|Full Text »|PDF »
NARF, an Nemo-like Kinase (NLK)-associated Ring Finger Protein Regulates the Ubiquitylation and Degradation of T Cell Factor/Lymphoid Enhancer Factor (TCF/LEF).
M. Yamada, J. Ohnishi, B. Ohkawara, S. Iemura, K. Satoh, J. Hyodo-Miura, K. Kawachi, T. Natsume, and H. Shibuya (2006)
J. Biol. Chem.
281, 20749-20760
|Abstract »|Full Text »|PDF »
MafG Sumoylation Is Required for Active Transcriptional Repression.
H. Motohashi, F. Katsuoka, C. Miyoshi, Y. Uchimura, H. Saitoh, C. Francastel, J. D. Engel, and M. Yamamoto (2006)
Mol. Cell. Biol.
26, 4652-4663
|Abstract »|Full Text »|PDF »
The myocardin family of transcriptional coactivators: versatile regulators of cell growth, migration, and myogenesis..
G.C. T. Pipes, E. E. Creemers, and E. N. Olson (2006)
Genes & Dev.
20, 1545-1556
|Abstract »|Full Text »|PDF »
Characterization of a Family of Nucleolar SUMO-specific Proteases with Preference for SUMO-2 or SUMO-3.
CA150 expression delays striatal cell death in overexpression and knock-in conditions for mutant huntingtin neurotoxicity..
M. Arango, S. Holbert, D. Zala, E. Brouillet, J. Pearson, E. Regulier, A. K. Thakur, P. Aebischer, R. Wetzel, N. Deglon, et al. (2006)
J. Neurosci.
26, 4649-4659
|Abstract »|Full Text »|PDF »
SUMOylation Interferes with CCAAT/Enhancer-Binding Protein beta-Mediated c-myc Repression, but Not IL-4 Activation in T Cells..
F. Berberich-Siebelt, I. Berberich, M. Andrulis, B. Santner-Nanan, M. K. Jha, S. Klein-Hessling, A. Schimpl, and E. Serfling (2006)
J. Immunol.
176, 4843-4851
|Abstract »|Full Text »|PDF »
PIAS proteins and transcriptional regulation--more than just SUMO E3 ligases?.
A. D. Sharrocks (2006)
Genes & Dev.
20, 754-758
|Full Text »|PDF »
PIAS1 confers DNA-binding specificity on the Msx1 homeoprotein..
H. Lee, J. C. Quinn, K. V. Prasanth, V. A. Swiss, K. D. Economides, M. M. Camacho, D. L. Spector, and C. Abate-Shen (2006)
Genes & Dev.
20, 784-794
|Abstract »|Full Text »|PDF »
SUMO-1 Controls the Protein Stability and the Biological Function of Phosducin.
C. Klenk, J. Humrich, U. Quitterer, and M. J. Lohse (2006)
J. Biol. Chem.
281, 8357-8364
|Abstract »|Full Text »|PDF »
SUMO-3 Enhances Androgen Receptor Transcriptional Activity through a Sumoylation-independent Mechanism in Prostate Cancer Cells.
Z. Zheng, C. Cai, J. Omwancha, S.-Y. Chen, T. Baslan, and L. Shemshedini (2006)
J. Biol. Chem.
281, 4002-4012
|Abstract »|Full Text »|PDF »
Control of MEF2 Transcriptional Activity by Coordinated Phosphorylation and Sumoylation.
S. Gregoire, A. M. Tremblay, L. Xiao, Q. Yang, K. Ma, J. Nie, Z. Mao, Z. Wu, V. Giguere, and X.-J. Yang (2006)
J. Biol. Chem.
281, 4423-4433
|Abstract »|Full Text »|PDF »
HIC-5 Is a Novel Repressor of Lymphoid Enhancer Factor/T-cell Factor-driven Transcription.
S. M. Ghogomu, S. van Venrooy, M. Ritthaler, D. Wedlich, and D. Gradl (2006)
J. Biol. Chem.
281, 1755-1764
|Abstract »|Full Text »|PDF »
Functional role of NF-IL6{beta} and its sumoylation and acetylation modifications in promoter activation of cyclooxygenase 2 gene.
Interaction of Moloney Murine Leukemia Virus Capsid with Ubc9 and PIASy Mediates SUMO-1 Addition Required Early in Infection.
A. Yueh, J. Leung, S. Bhattacharyya, L. A. Perrone, K. de los Santos, S.-y. Pu, and S. P. Goff (2006)
J. Virol.
80, 342-352
|Abstract »|Full Text »|PDF »
FLI-1 Functionally Interacts with PIASx{alpha}, a Member of the PIAS E3 SUMO Ligase Family.
E. van den Akker, S. Ano, H.-M. Shih, L.-C. Wang, M. Pironin, J. J. Palvimo, N. Kotaja, O. Kirsh, A. Dejean, and J. Ghysdael (2005)
J. Biol. Chem.
280, 38035-38046
|Abstract »|Full Text »|PDF »
Sumoylation of the Estrogen Receptor {alpha} Hinge Region Regulates Its Transcriptional Activity.
S. Sentis, M. Le Romancer, C. Bianchin, M.-C. Rostan, and L. Corbo (2005)
Mol. Endocrinol.
19, 2671-2684
|Abstract »|Full Text »|PDF »
Interactions between Coilin and PIASy partially link Cajal bodies to PML bodies.
J. Sun, H. Xu, S. H. Subramony, and M. D. Hebert (2005)
J. Cell Sci.
118, 4995-5003
|Abstract »|Full Text »|PDF »
J. M.P. Desterro, L. P. Keegan, E. Jaffray, R. T. Hay, M. A. O'Connell, and M. Carmo-Fonseca (2005)
Mol. Biol. Cell
16, 5115-5126
|Abstract »|Full Text »|PDF »
TRAF7 Sequesters c-Myb to the Cytoplasm by Stimulating Its Sumoylation.
Y. Morita, C. Kanei-Ishii, T. Nomura, and S. Ishii (2005)
Mol. Biol. Cell
16, 5433-5444
|Abstract »|Full Text »|PDF »
Pc2-mediated Sumoylation of Smad-interacting Protein 1 Attenuates Transcriptional Repression of E-cadherin.
Regulation of MEF2 by Histone Deacetylase 4- and SIRT1 Deacetylase-Mediated Lysine Modifications.
X. Zhao, T. Sternsdorf, T. A. Bolger, R. M. Evans, and T.-P. Yao (2005)
Mol. Cell. Biol.
25, 8456-8464
|Abstract »|Full Text »|PDF »
An internal ribosome entry site mediates translation of lymphoid enhancer factor-1.
J. JIMENEZ, G. M. JANG, B. L. SEMLER, and M. L. WATERMAN (2005)
RNA
11, 1385-1399
|Abstract »|Full Text »|PDF »
Down-Regulation of c-Fos/c-Jun AP-1 Dimer Activity by Sumoylation.
G. Bossis, C. E. Malnou, R. Farras, E. Andermarcher, R. Hipskind, M. Rodriguez, D. Schmidt, S. Muller, I. Jariel-Encontre, and M. Piechaczyk (2005)
Mol. Cell. Biol.
25, 6964-6979
|Abstract »|Full Text »|PDF »
SUMO-Dependent Compartmentalization in Promyelocytic Leukemia Protein Nuclear Bodies Prevents the Access of LRH-1 to Chromatin.
Quantitative SUMO-1 Modification of a Vaccinia Virus Protein Is Required for Its Specific Localization and Prevents Its Self-Association.
S. Palacios, L. H. Perez, S. Welsch, S. Schleich, K. Chmielarska, F. Melchior, and J. K. Locker (2005)
Mol. Biol. Cell
16, 2822-2835
|Abstract »|Full Text »|PDF »
SUMO Represses Transcriptional Activity of the Drosophila SoxNeuro and Human Sox3 Central Nervous System-specific Transcription Factors.
J. Savare, N. Bonneaud, and F. Girard (2005)
Mol. Biol. Cell
16, 2660-2669
|Abstract »|Full Text »|PDF »
Sumoylation induced by the Arf tumor suppressor: A p53-independent function.
The DEAD-Box Protein DP103 (Ddx20 or Gemin-3) Represses Orphan Nuclear Receptor Activity via SUMO Modification.
M. B. Lee, L. A. Lebedeva, M. Suzawa, S. A. Wadekar, M. Desclozeaux, and H. A. Ingraham (2005)
Mol. Cell. Biol.
25, 1879-1890
|Abstract »|Full Text »|PDF »
Role for SUMO Modification in Facilitating Transcriptional Repression by BKLF.
J. Perdomo, A. Verger, J. Turner, and M. Crossley (2005)
Mol. Cell. Biol.
25, 1549-1559
|Abstract »|Full Text »|PDF »
Negative Regulation of NF-{kappa}B Signaling by PIAS1.
B. Liu, R. Yang, K. A. Wong, C. Getman, N. Stein, M. A. Teitell, G. Cheng, H. Wu, and K. Shuai (2005)
Mol. Cell. Biol.
25, 1113-1123
|Abstract »|Full Text »|PDF »
Sumoylation of MITF and Its Related Family Members TFE3 and TFEB.
A. J. Miller, C. Levy, I. J. Davis, E. Razin, and D. E. Fisher (2005)
J. Biol. Chem.
280, 146-155
|Abstract »|Full Text »|PDF »
p53-Dependent and -Independent Functions of the Arf Tumor Suppressor.
C.J. SHERR, D. BERTWISTLE, W. DEN BESTEN, M.-L. KUO, M. SUGIMOTO, K. TAGO, R.T. WILLIAMS, F. ZINDY, and M.F. ROUSSEL (2005)
Cold Spring Harb Symp Quant Biol
70, 129-137
|Abstract »|PDF »
A Universal Strategy for Proteomic Studies of SUMO and Other Ubiquitin-like Modifiers.
G. Rosas-Acosta, W. K. Russell, A. Deyrieux, D. H. Russell, and V. G. Wilson (2005)
Mol. Cell. Proteomics
4, 56-72
|Abstract »|Full Text »|PDF »
Regulation and Function of SUMO Modification.
R. S. Hilgarth, L. A. Murphy, H. S. Skaggs, D. C. Wilkerson, H. Xing, and K. D. Sarge (2004)
J. Biol. Chem.
279, 53899-53902
|Full Text »|PDF »
Nuclear translocation of an ICA512 cytosolic fragment couples granule exocytosis and insulin expression in {beta}-cells.
M. Trajkovski, H. Mziaut, A. Altkruger, J. Ouwendijk, K.-P. Knoch, S. Muller, and M. Solimena (2004)
J. Cell Biol.
167, 1063-1074
|Abstract »|Full Text »|PDF »
PIAS-1 Is a Checkpoint Regulator Which Affects Exit from G1 and G2 by Sumoylation of p73.
E. Munarriz, D. Barcaroli, A. Stephanou, P. A. Townsend, C. Maisse, A. Terrinoni, M. H. Neale, S. J. Martin, D. S. Latchman, R. A. Knight, et al. (2004)
Mol. Cell. Biol.
24, 10593-10610
|Abstract »|Full Text »|PDF »
p14 Arf Promotes Small Ubiquitin-like Modifier Conjugation of Werners Helicase.
Y. L. Woods, D. P. Xirodimas, A. R. Prescott, A. Sparks, D. P. Lane, and M. K. Saville (2004)
J. Biol. Chem.
279, 50157-50166
|Abstract »|Full Text »|PDF »
J. Wang, X.-h. Feng, and R. J. Schwartz (2004)
J. Biol. Chem.
279, 49091-49098
|Abstract »|Full Text »|PDF »
PIASy-Deficient Mice Display Modest Defects in IFN and Wnt Signaling.
W. Roth, C. Sustmann, M. Kieslinger, A. Gilmozzi, D. Irmer, E. Kremmer, C. Turck, and R. Grosschedl (2004)
J. Immunol.
173, 6189-6199
|Abstract »|Full Text »|PDF »
Negative regulation of Smad2 by PIASy is required for proper Xenopus mesoderm formation.
M. Daniels, K. Shimizu, A. M. Zorn, and S.-i. Ohnuma (2004)
Development
131, 5613-5626
|Abstract »|Full Text »|PDF »
Drosophila Ulp1, a Nuclear Pore-associated SUMO Protease, Prevents Accumulation of Cytoplasmic SUMO Conjugates.
M. Smith, V. Bhaskar, J. Fernandez, and A. J. Courey (2004)
J. Biol. Chem.
279, 43805-43814
|Abstract »|Full Text »|PDF »
-catenin and repress transcription in association with Groucho. In search of additional regulatory mechanisms of LEF1 function, we identified the protein inhibitor of
activated STAT, PIASy, as a novel interaction partner of LEF1. Coexpression of PIASy with LEF1 results in potent repression of LEF1
activity and in covalent modification of LEF1 with SUMO. PIASy markedly
stimulates the sumoylation of LEF1 and multiple other proteins in vivo
and functions as a SUMO E3 ligase for LEF1 in a reconstituted system in
vitro. Moreover, PIASy binds to nuclear matrix-associated DNA
sequences and targets LEF1 to nuclear bodies, suggesting that PIASy-mediated
subnuclear sequestration accounts for the repression of LEF1 activity.
