Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Subscribe

Logo for

J. Biol. Chem. 275 (39): 30520-30524

© 2000 by The American Society for Biochemistry and Molecular Biology, Inc.

A Role for Nuclear Phospholipase Cbeta 1 in Cell Cycle Control*

Irene FaenzaDagger , Alessandro Matteucci§, Lucia ManzoliDagger , Anna Maria BilliDagger , Michela AluigiDagger , Daniela PeruzziDagger , Marco Vitale, Sergio Castorina||, Pann-Ghill Suh**, and Lucio CoccoDagger Dagger Dagger

From the Dagger  Department of Anatomical Sciences, Cellular Signalling Laboratory, University of Bologna, Via Irnerio 48, I-40126 Bologna, Italy the § Institute of Cytomorphology, CNR c/o Rizzoli Institute, I-40136 Bologna, Italy, the  Department of Biomedical Sciences and Biotechnology, University of Brescia, I-25123 Brescia, Italy, the || Department of Anatomy, University of Catania, I-95124 Catania, Italy, and the ** Department of Life Sciences, Division of Molecular Life Science, Pohang University of Science and Technology, 633-165 Pohang, South Korea

Phosphoinositide signaling resides in the nucleus, and among the enzymes of the cycle, phospholipase C (PLC) appears as the key element both in Saccharomyces cerevisiae and in mammalian cells. The yeast PLC pathway produces multiple inositol polyphosphates that modulate distinct nuclear processes. The mammalian PLCbeta 1, which localizes in the nucleus, is activated in insulin-like growth factor 1-mediated mitogenesis and undergoes down-regulation during murine erythroleukemia differentiation. PLCbeta 1 exists as two polypeptides of 150 and 140 kDa generated from a single gene by alternative RNA splicing, both of them containing in the COOH-terminal tail a cluster of lysine residues responsible for nuclear localization. These clues prompted us to try to establish the critical nuclear target(s) of PLCbeta 1 subtypes in the control of cell cycle progression. The results reveal that the two subtypes of PLCbeta 1 that localize in the nucleus induce cell cycle progression in Friend erythroleukemia cells. In fact when they are overexpressed in the nucleus, cyclin D3, along with its kinase (cdk4) but not cyclin E is overexpressed even though cells are serum-starved. As a consequence of this enforced expression, retinoblastoma protein is phosphorylated and E2F-1 transcription factor is activated as well. On the whole the results reveal a direct effect of nuclear PLCbeta 1 signaling in G1 progression by means of a specific target, i.e. cyclin D3/cdk4.

* This work was supported by the Italian Association for Cancer Research, Italian Murst Cofin99, Selected Topics Research Fund of Bologna University, and Italian CNR PF Biotechnology grants.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger Dagger To whom correspondence should be addressed. E-mail: lcocco@biocfarm.unibo.it.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Distinct Phospholipase C-{beta} Isozymes Mediate Lysophosphatidic Acid Receptor 1 Effects on Intestinal Epithelial Homeostasis and Wound Closure.
S.-J. Lee, G. Leoni, P.-A. Neumann, J. Chun, A. Nusrat, and C. C. Yun (2013)
Mol. Cell. Biol. 33, 2016-2028
   Abstract »    Full Text »    PDF »
Inositol Pyrophosphates Modulate S Phase Progression after Pheromone-induced Arrest in Saccharomyces cerevisiae.
H. Banfic, A. Bedalov, J. D. York, and D. Visnjic (2013)
J. Biol. Chem. 288, 1717-1725
   Abstract »    Full Text »    PDF »
A role for PLC{beta}1 in myotonic dystrophies type 1 and 2.
I. Faenza, W. Blalock, A. Bavelloni, B. Schoser, R. Fiume, S. Pacella, M. Piazzi, A. D'Angelo, and L. Cocco (2012)
FASEB J 26, 3042-3048
   Abstract »    Full Text »    PDF »
Phospholipase C Beta 1 Expression in the Dorsolateral Prefrontal Cortex from Patients with Schizophrenia at Different Stages of Illness.
M. Udawela, E. Scarr, A. J. Hannan, E. A. Thomas, and B. Dean (2011)
Australian and New Zealand Journal of Psychiatry 45, 140-147
   Abstract »    Full Text »    PDF »
eEF1A Phosphorylation in the Nucleus of Insulin-stimulated C2C12 Myoblasts: SER53 IS A NOVEL SUBSTRATE FOR PROTEIN KINASE C {beta}I.
M. Piazzi, A. Bavelloni, I. Faenza, W. Blalock, A. Urbani, S. D'Aguanno, R. Fiume, G. Ramazzotti, N. M. Maraldi, and L. Cocco (2010)
Mol. Cell. Proteomics 9, 2719-2728
   Abstract »    Full Text »    PDF »
Gq-initiated cardiomyocyte hypertrophy is mediated by phospholipase C{beta}1b.
T. M. Filtz, D. R. Grubb, T. J. McLeod-Dryden, J. Luo, and E. A. Woodcock (2009)
FASEB J 23, 3564-3570
   Abstract »    Full Text »    PDF »
Reduction of phosphoinositide-phospholipase C beta1 methylation predicts the responsiveness to azacitidine in high-risk MDS.
M. Y. Follo, C. Finelli, S. Mongiorgi, C. Clissa, C. Bosi, N. Testoni, F. Chiarini, G. Ramazzotti, M. Baccarani, A. M. Martelli, et al. (2009)
PNAS 106, 16811-16816
   Abstract »    Full Text »    PDF »
Involvement of nuclear PLC{beta}1 in lamin B1 phosphorylation and G2/M cell cycle progression.
R. Fiume, G. Ramazzotti, G. Teti, F. Chiarini, I. Faenza, G. Mazzotti, A. M. Billi, and L. Cocco (2009)
FASEB J 23, 957-966
   Abstract »    Full Text »    PDF »
Phosphoinositide-Phospholipase C {beta}1 Mono-Allelic Deletion Is Associated With Myelodysplastic Syndromes Evolution Into Acute Myeloid Leukemia.
M. Y. Follo, C. Finelli, C. Clissa, S. Mongiorgi, C. Bosi, G. Martinelli, M. Baccarani, L. Manzoli, A. M. Martelli, and L. Cocco (2009)
J. Clin. Oncol. 27, 782-790
   Abstract »    Full Text »    PDF »
The extreme C-terminal region of phospholipase C{beta}1 determines subcellular localization and function; the "b" splice variant mediates {alpha}1-adrenergic receptor responses in cardiomyocytes.
D. R. Grubb, O. Vasilevski, H. Huynh, and E. A. Woodcock (2008)
FASEB J 22, 2768-2774
   Abstract »    Full Text »    PDF »
Phospholipase C-{delta}1 Expression Is Linked to Proliferation, DNA Synthesis, and Cyclin E Levels.
J. D. Stallings, Y. X. Zeng, F. Narvaez, and M. J. Rebecchi (2008)
J. Biol. Chem. 283, 13992-14001
   Abstract »    Full Text »    PDF »
Interaction of the melanocortin 2 receptor with nucleoporin 50: evidence for a novel pathway between a G-protein-coupled receptor and the nucleus.
M. Doufexis, H. L. Storr, P. J. King, and A. J. L. Clark (2007)
FASEB J 21, 4095-4100
   Abstract »    Full Text »    PDF »
Phospholipase C-{beta}2 promotes mitosis and migration of human breast cancer-derived cells.
V. Bertagnolo, M. Benedusi, F. Brugnoli, P. Lanuti, M. Marchisio, P. Querzoli, and S. Capitani (2007)
Carcinogenesis 28, 1638-1645
   Abstract »    Full Text »    PDF »
Inositide-Dependent Phospholipase C Signaling Mimics Insulin in Skeletal Muscle Differentiation by Affecting Specific Regions of the Cyclin D3 Promoter.
I. Faenza, G. Ramazzotti, A. Bavelloni, R. Fiume, G. C. Gaboardi, M. Y. Follo, R. S. Gilmour, A. M. Martelli, K. Ravid, and L. Cocco (2007)
Endocrinology 148, 1108-1117
   Abstract »    Full Text »    PDF »
Type 1 Parathyroid Hormone Receptor (PTH1R) Nuclear Trafficking: Association of PTH1R with Importin {alpha}1 and {beta}.
B. W. Pickard, A. B. Hodsman, L. J. Fraher, and P. H. Watson (2006)
Endocrinology 147, 3326-3332
   Abstract »    Full Text »    PDF »
The Retinoblastoma Family Proteins Bind to and Activate Diacylglycerol Kinase{zeta}.
A. P. Los, F. P. Vinke, J. de Widt, M. K. Topham, W. J. van Blitterswijk, and N. Divecha (2006)
J. Biol. Chem. 281, 858-866
   Abstract »    Full Text »    PDF »
Cyclosporin A Specifically Affects Nuclear PLC{beta}1 in Immunodepressed Heart Transplant Patients with Gingival Overgrowth.
A. Ruggeri Jr., L. Montebugnoli, A. Matteucci, N. Zini, L. Solimando, D. Servidio, P. Suppa, M. Cadenaro, L. Cocco, and L. Breschi (2005)
Journal of Dental Research 84, 747-751
   Abstract »    Full Text »    PDF »
Nuclear Phospholipase C {beta}1 (PLC{beta}1) Affects CD24 Expression in Murine Erythroleukemia Cells.
R. Fiume, I. Faenza, A. Matteucci, A. Astolfi, M. Vitale, A. M. Martelli, and L. Cocco (2005)
J. Biol. Chem. 280, 24221-24226
   Abstract »    Full Text »    PDF »
Dynamics of Dendritic Cell Phenotype and Interactions with CD4+ T Cells in Airway Inflammation and Tolerance.
T. B. Oriss, M. Ostroukhova, C. Seguin-Devaux, B. Dixon-McCarthy, D. B. Stolz, S. C. Watkins, B. Pillemer, P. Ray, and A. Ray (2005)
J. Immunol. 174, 854-863
   Abstract »    Full Text »    PDF »
Agonist-independent Nuclear Localization of the Apelin, Angiotensin AT1, and Bradykinin B2 Receptors.
D. K. Lee, A. J. Lanca, R. Cheng, T. Nguyen, X. D. Ji, F. Gobeil Jr., S. Chemtob, Susan. R. George, and B. F. O'Dowd (2004)
J. Biol. Chem. 279, 7901-7908
   Abstract »    Full Text »    PDF »
Functional Endothelin Receptors Are Present on Nuclei in Cardiac Ventricular Myocytes.
B. Boivin, D. Chevalier, L. R. Villeneuve, E. Rousseau, and B. G. Allen (2003)
J. Biol. Chem. 278, 29153-29163
   Abstract »    Full Text »    PDF »
Ca2+ oscillations at fertilization in mammals are regulated by the formation of pronuclei.
P. Marangos, G. FitzHarris, and J. Carroll (2003)
Development 130, 1461-1472
   Abstract »    Full Text »    PDF »
Nuclear Lipid Signaling.
R. F. Irvine (2002)
Sci. STKE 2002, re13
   Abstract »    Full Text »    PDF »
Phosphorylation of Nuclear Phospholipase C {beta}1 by Extracellular Signal-Regulated Kinase Mediates the Mitogenic Action of Insulin-Like Growth Factor I.
A. Xu, P.-G. Suh, N. Marmy-Conus, R. B. Pearson, O. Y. Seok, L. Cocco, and R. S. Gilmour (2001)
Mol. Cell. Biol. 21, 2981-2990
   Abstract »    Full Text »    PDF »
Protein Kinase C alpha -mediated Negative Feedback Regulation Is Responsible for the Termination of Insulin-like Growth Factor I-induced Activation of Nuclear Phospholipase C beta 1 in Swiss 3T3 Cells.
A. Xu, Y. Wang, L. Y. Xu, and R. S. Gilmour (2001)
J. Biol. Chem. 276, 14980-14986
   Abstract »    Full Text »    PDF »
Evidence for Selective Coupling of alpha 1-Adrenergic Receptors to Phospholipase C-beta 1 in Rat Neonatal Cardiomyocytes.
J. F. Arthur, S. J. Matkovich, C. J. Mitchell, T. J. Biden, and E. A. Woodcock (2001)
J. Biol. Chem. 276, 37341-37346
   Abstract »    Full Text »    PDF »

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882