Related Content
Search Google Scholar for:
|
J. Biol. Chem. 275 (9): 6063-6066
© 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
J Biol Chem, Vol. 275, Issue 9, 6063-6066, March 3, 2000
ACCELERATED PUBLICATION
Inositol Tetrakisphosphate as a Frequency Regulator in Calcium
Oscillations in HeLa Cells*
De-Min
Zhu ,
Ephrem
Tekle,
Charles Y.
Huang, and
P. Boon
Chock§
From the Laboratory of Biochemistry, NHLBI, National Institutes of
Health, Bethesda, Maryland 20892
Cellular signaling mediated by inositol
(1,4,5)trisphosphate (Ins(1,4,5)P3) results in
oscillatory intracellular calcium (Ca2+) release. Because
the amplitude of the Ca2+ spikes is relatively invariant,
the extent of the agonist-mediated effects must reside in their ability
to regulate the oscillating frequency. Using electroporation
techniques, we show that Ins(1,4,5)P3, Ins(1,3,4,5)P4, and Ins(1,3,4,6)P4 cause a
rapid intracellular Ca2+ release in resting HeLa cells and
a transient increase in the frequency of ongoing Ca2+
oscillations stimulated by histamine. Two poorly metabolizable analogs
of Ins(1,4,5)P3, Ins(2,4,5)P3, and
2,3-dideoxy-Ins(1,4,5)P3, gave a single Ca2+
spike and failed to alter the frequency of ongoing oscillations. Complete inhibition of Ins(1,4,5)P3 3-kinase (IP3K) by
either adriamycin or its specific antibody blocked Ca2+
oscillations. Partial inhibition of IP3K causes a significant reduction
in frequency. Taken together, our results indicate that Ins(1,3,4,5)P4 is the frequency regulator in
vivo, and IP3K, which phosphorylates Ins(1,4,5)P3 to
Ins(1,3,4,5)P4, plays a major regulatory role in
intracellular Ca2+ oscillations.
*
The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Present address: Immunology Dept., Wayne Hughes Institute, 2665 Long Lake Rd., St. Paul, MN 55113.
§
To whom correspondence should be addressed: Laboratory of
Biochemistry, NHLBI, National Institutes of Health, Bldg. 3, Rm. 204, 3 Center Dr., MSC-0342, Bethesda, MD 20892-0342. Tel.: 301-496-2073; Fax:
301-496-0599. E-mail: pbc@helix.nih.gov.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
- Headpiece Domain of Dematin Regulates Calcium Mobilization and Signaling in Platelets.
- A. J. Wieschhaus, G. C. Le Breton, and A. H. Chishti (2012)
J. Biol. Chem.
287, 41218-41231
| Abstract »
| Full Text »
| PDF »
- At5PTase13 Modulates Cotyledon Vein Development through Regulating Auxin Homeostasis.
- W.-H. Lin, Y. Wang, B. Mueller-Roeber, C. A. Brearley, Z.-H. Xu, and H.-W. Xue (2005)
Plant Physiology
139, 1677-1691
| Abstract »
| Full Text »
| PDF »
- Inositol (1,4,5) trisphosphate 3 kinase B controls positive selection of T cells and modulates Erk activity.
- B. G. Wen, M. T. Pletcher, M. Warashina, S. H. Choe, N. Ziaee, T. Wiltshire, K. Sauer, and M. P. Cooke (2004)
PNAS
101, 5604-5609
| Abstract »
| Full Text »
| PDF »
- Monitoring Agonist-induced Phospholipase C Activation in Live Cells by Fluorescence Resonance Energy Transfer.
- J. van der Wal, R. Habets, P. Varnai, T. Balla, and K. Jalink (2001)
J. Biol. Chem.
276, 15337-15344
| Abstract »
| Full Text »
| PDF »
|
|
