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J. Biol. Chem. 276 (11): 7701-7704

© 2001 by The American Society for Biochemistry and Molecular Biology, Inc.

I{kappa}B Family Members Function by Different Mechanisms*

Winnie F. Tam, and Ranjan Sen{ddagger}

From the Rosenstiel Basic Medical Sciences Research Center and the Department of Biology, Brandeis University, Waltham, Massachusetts 02454

ABSTRACT Back to Top

Abstract: The I{kappa}B family of proteins regulates NF-{kappa}B-dependent transcription by inhibiting DNA binding and localizing these factors to the cell cytoplasm. I{kappa}Bα does this by shifting the balance between nuclear import of Rel proteins and their export from the nucleus. Here we show that, unlike I{kappa}Bα, I{kappa}Bβ and I{kappa}B{varepsilon} appear to sequester p65 or c-Rel in the cytoplasm by inhibiting nuclear import. Furthermore, because I{kappa}Bβ does not undergo nucleocytoplasmic shuttling, it cannot remove nuclear proteins like I{kappa}Bα does. We conclude that the mechanism of action differs among I{kappa}B family members.


Received for publication December 22, 2000.

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