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J. Biol. Chem. 276 (27): 24441-24444

© 2001 by The American Society for Biochemistry and Molecular Biology, Inc.

The C2A Domain of Double C2 Protein gamma  Contains a Functional Nuclear Localization Signal*

Mitsunori FukudaDagger §, Chika SaegusaDagger , Eiko KannoDagger , and Katsuhiko MikoshibaDagger

From the Dagger  Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN (the Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako, Saitama 351-0198 and the  Division of Molecular Neurobiology, Department of Basic Medical Science, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan

The C2 domain was originally defined as a homologous domain to the C2 regulatory region of Ca2+-dependent protein kinase C and has been identified in more than 50 different signaling molecules. The original C2 domain of protein kinase Calpha functions as a Ca2+ binding module, and the Ca2+ binding to the C2 domain allows translocation of proteins to phospholipid membranes. By contrast, however, some C2 domains do not exhibit Ca2+ binding activity because of amino acid substitutions at Ca2+-binding sites, and their physiological meanings remain largely unknown. In this study, we discovered an unexpected function of the Ca2+-independent C2A domain of double C2 protein gamma  (Doc2gamma ) in nuclear localization. Deletion and mutation analyses revealed that the putative Ca2+ binding loop 3 of Doc2gamma contains six Arg residues (177RLRRRRR183) and that this basic cluster is both necessary and sufficient for nuclear localization of Doc2gamma . Because of the presence of the basic cluster, the C2A domain of Doc2gamma did not show Ca2+-dependent phospholipid binding activity. Our findings indicate that by changing the nature of the putative Ca2+ binding loops the C2 domain has more diversified function in cellular signaling than a simple Ca2+ binding motif.

* This work was supported in part by grants from the Science and Technology Agency of Japan (to K. M.) and by Grants 11780571 and 12053274 from the Ministry of Education, Science, and Culture of Japan (to M. F.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ To whom correspondence should be addressed. Tel.: 81-48-467-9745; Fax: 81-48-467-9744; E-mail:

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.

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