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© 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Odorants Stimulate the ERK/Mitogen-activated Protein Kinase Pathway and Activate cAMP-response Element-mediated Transcription in Olfactory Sensory Neurons* and
From the Department of Pharmacology, University of Washington,
Seattle, Washington 98195-7280
Olfactory sensory neurons (OSNs) respond acutely
to volatile molecules and exhibit adaptive responses including
desensitization to odorant exposure. Although mechanisms for short term
adaptation have been described, there is little evidence that odorants
cause long lasting, transcription-dependent changes in
OSNs. Here we report that odorants stimulate cAMP-response element
(CRE)-mediated transcription in OSNs through Ca2+
activation of the ERK/MAPK/p90rsk pathway. Odorant stimulation
of ERK phosphorylation was ablated by inhibition of
calmodulin-dependent protein kinase II suggesting that
odorant activation of ERK is mediated through this kinase. Moreover, a
brief exposure in vivo to an odorant in vapor phase stimulated CRE-mediated gene transcription in discrete populations of
OSNs. These data suggest that like central nervous system neurons, OSNs
may undergo long term adaptive changes mediated through CRE-mediated transcription.
* This work was supported in part by National Institutes of Health Grants DC04156 and NS357056.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Supported by National Institutes of Health Training Grant GM07270.
§
To whom correspondence should be addressed: Dept. of Pharmacology,
Mail Box 357280, Health Sciences Bldg., University of Washington, Seattle, WA 98195-7280. Tel.: 206-543-7028; Fax: 206-616-8621; E-mail: dstorm@u.washington.edu.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc. THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882
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