Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Logo for

J. Biol. Chem. 276 (4): 2752-2757

© 2001 by The American Society for Biochemistry and Molecular Biology, Inc.

Regulation of a Novel Human Phospholipase C, PLCepsilon , through Membrane Targeting by Ras*

Chunhua SongDagger §, Chang-Deng HuDagger §, Misa MasagoDagger , Ken-ichi Kariya, Yuriko Yamawaki-KataokaDagger , Mitsushige ShibatohgeDagger , Dongmei WuDagger , Takaya SatohDagger , and Tohru KataokaDagger ||

From the Dagger  Department of Physiology II, Kobe University School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan and the  Department of Biochemistry II, School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan

Phosphoinositide-specific phospholipase C (PI-PLC) plays a pivotal role in regulation of intracellular signal transduction from various receptor molecules. More than 10 members of human PI-PLC isoforms have been identified and classified into three classes beta , gamma , and delta , which are regulated by distinct mechanisms. Here we report identification of a novel class of human PI-PLC, named PLCepsilon , which is characterized by the presence of a Ras-associating domain at its C terminus and a CDC25-like domain at its N terminus. The Ras-associating domain of PLCepsilon specifically binds to the GTP-bound forms of Ha-Ras and Rap1A. The dissociation constant for Ha-Ras is estimated to be approximately 40 nM, comparable with those of other Ras effectors. Co-expression of an activated Ha-Ras mutant with PLCepsilon induces its translocation from the cytosol to the plasma membrane. Upon stimulation with epidermal growth factor, similar translocation of ectopically expressed PLCepsilon is observed, which is inhibited by co-expression of dominant-negative Ha-Ras. Furthermore, using a liposome-based reconstitution assay, it is shown that the phosphatidylinositol 4,5-bisphosphate-hydrolyzing activity of PLCepsilon is stimulated in vitro by Ha-Ras in a GTP-dependent manner. These results indicate that Ras directly regulates phosphoinositide breakdown through membrane targeting of PLCepsilon .

* This work was supported by Ministry of Education, Science, Sports and Culture of Japan Grants 11470034, 12215098, 12670116, and 12670136 and by funds from the Sankyo Foundation of Life Science, Hyogo Science and Technology Association, and Kobe University.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF190642.

§ These authors contributed equally to this work.

|| To whom correspondence should be addressed. Tel.: 81-78-382-5380; Fax: 81-78-382-5399; E-mail:

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.

Association between phospholipase C epsilon gene (PLCE1) polymorphism and colorectal cancer risk in a Chinese population.
Q. Wang, P. Chen, D. Chen, F. Liu, and W. Pan (2014)
Journal of International Medical Research 42, 270-281
   Abstract »    Full Text »    PDF »
Phosphoinositides: Tiny Lipids With Giant Impact on Cell Regulation.
T. Balla (2013)
Physiol Rev 93, 1019-1137
   Abstract »    Full Text »    PDF »
PLC{zeta} causes Ca2+ oscillations in mouse eggs by targeting intracellular and not plasma membrane PI(4,5)P2.
Y. Yu, M. Nomikos, M. Theodoridou, G. Nounesis, F. A. Lai, and K. Swann (2012)
Mol. Biol. Cell 23, 371-380
   Abstract »    Full Text »    PDF »
Phospholipase C{epsilon} Scaffolds to Muscle-specific A Kinase Anchoring Protein (mAKAP{beta}) and Integrates Multiple Hypertrophic Stimuli in Cardiac Myocytes.
L. Zhang, S. Malik, G. G. Kelley, M. S. Kapiloff, and A. V. Smrcka (2011)
J. Biol. Chem. 286, 23012-23021
   Abstract »    Full Text »    PDF »
Therapeutic Strategies for Targeting Ras Proteins.
S. Gysin, M. Salt, A. Young, and F. McCormick (2011)
Genes & Cancer 2, 359-372
   Abstract »    Full Text »    PDF »
Dual Regulation of RA-RhoGAP Activity by Phosphatidic Acid and Rap1 during Neurite Outgrowth.
T. Kurooka, Y. Yamamoto, Y. Takai, and T. Sakisaka (2011)
J. Biol. Chem. 286, 6832-6843
   Abstract »    Full Text »    PDF »
Epac2-dependent mobilization of intracellular Ca2+ by glucagon-like peptide-1 receptor agonist exendin-4 is disrupted in {beta}-cells of phospholipase C- knockout mice.
I. Dzhura, O. G. Chepurny, G. G. Kelley, C. A. Leech, M. W. Roe, E. Dzhura, P. Afshari, S. Malik, M. J. Rindler, X. Xu, et al. (2010)
J. Physiol. 588, 4871-4889
   Abstract »    Full Text »    PDF »
Enhancement of ultraviolet B-induced skin tumor development in phospholipase C{varepsilon}-knockout mice is associated with decreased cell death.
M. Oka, H. Edamatsu, M. Kunisada, L. Hu, N. Takenaka, S. Dien, M. Sakaguchi, R. Kitazawa, K. Norose, T. Kataoka, et al. (2010)
Carcinogenesis 31, 1897-1902
   Abstract »    Full Text »    PDF »
{beta}-Arrestin-dependent activation of Ca2+/calmodulin kinase II after {beta}1-adrenergic receptor stimulation.
S. Mangmool, A. K. Shukla, and H. A. Rockman (2010)
J. Cell Biol. 189, 573-587
   Abstract »    Full Text »    PDF »
Crucial Role of Phospholipase C{varepsilon} in Induction of Local Skin Inflammatory Reactions in the Elicitation Stage of Allergic Contact Hypersensitivity.
L. Hu, H. Edamatsu, N. Takenaka, S. Ikuta, and T. Kataoka (2010)
J. Immunol. 184, 993-1002
   Abstract »    Full Text »    PDF »
Roles of Phospholipase C Isozymes in Organogenesis and Embryonic Development.
Y. Nakamura and K. Fukami (2009)
Physiology 24, 332-341
   Abstract »    Full Text »    PDF »
Ras is an indispensable coregulator of the class IB phosphoinositide 3-kinase p87/p110{gamma}.
B. Kurig, A. Shymanets, T. Bohnacker, Prajwal, C. Brock, M. R. Ahmadian, M. Schaefer, A. Gohla, C. Harteneck, M. P. Wymann, et al. (2009)
PNAS 106, 20312-20317
   Abstract »    Full Text »    PDF »
Phospholipase C{varepsilon} promotes intestinal tumorigenesis of ApcMin/+ mice through augmentation of inflammation and angiogenesis.
M. Li, H. Edamatsu, R. Kitazawa, S. Kitazawa, and T. Kataoka (2009)
Carcinogenesis 30, 1424-1432
   Abstract »    Full Text »    PDF »
Specificity in Ras and Rap Signaling.
J. H. Raaijmakers and J. L. Bos (2009)
J. Biol. Chem. 284, 10995-10999
   Abstract »    Full Text »    PDF »
Phospholipase C isozymes as effectors of Ras superfamily GTPases.
T. K. Harden, S. N. Hicks, and J. Sondek (2009)
J. Lipid Res. 50, S243-S248
   Abstract »    Full Text »    PDF »
Dual Activation of Phospholipase C-{epsilon} by Rho and Ras GTPases.
J. P. Seifert, Y. Zhou, S. N. Hicks, J. Sondek, and T. K. Harden (2008)
J. Biol. Chem. 283, 29690-29698
   Abstract »    Full Text »    PDF »
Rac Regulates Its Effector Phospholipase C{gamma}2 through Interaction with a Split Pleckstrin Homology Domain.
C. Walliser, M. Retlich, R. Harris, K. L. Everett, M. B. Josephs, P. Vatter, D. Esposito, P. C. Driscoll, M. Katan, P. Gierschik, et al. (2008)
J. Biol. Chem. 283, 30351-30362
   Abstract »    Full Text »    PDF »
Siah Proteins Induce the Epidermal Growth Factor-dependent Degradation of Phospholipase C{epsilon}.
S. Yun, A. Moller, S.-K. Chae, W.-P. Hong, Y. J. Bae, D. D. L. Bowtell, S. H. Ryu, and P.-G. Suh (2008)
J. Biol. Chem. 283, 1034-1042
   Abstract »    Full Text »    PDF »
Phospholipase C-{epsilon} Augments Epidermal Growth Factor-dependent Cell Growth by Inhibiting Epidermal Growth Factor Receptor Down-regulation.
S. Yun, W.-P. Hong, J. H. Choi, K. S. Yi, S.-K. Chae, S. H. Ryu, and P.-G. Suh (2008)
J. Biol. Chem. 283, 341-349
   Abstract »    Full Text »    PDF »
Crucial Role of Phospholipase C{varepsilon} in Skin Inflammation Induced by Tumor-Promoting Phorbol Ester.
S. Ikuta, H. Edamatsu, M. Li, L. Hu, and T. Kataoka (2008)
Cancer Res. 68, 64-72
   Abstract »    Full Text »    PDF »
Phospholipase C{varepsilon} is a nexus for Rho and Rap-mediated G protein-coupled receptor-induced astrocyte proliferation.
S. Citro, S. Malik, E. A. Oestreich, J. Radeff-Huang, G. G. Kelley, A. V. Smrcka, and J. H. Brown (2007)
PNAS 104, 15543-15548
   Abstract »    Full Text »    PDF »
The M-Ras-RA-GEF-2-Rap1 Pathway Mediates Tumor Necrosis Factor-{alpha}-dependent Regulation of Integrin Activation in Splenocytes.
Y. Yoshikawa, T. Satoh, T. Tamura, P. Wei, S. E. Bilasy, H. Edamatsu, A. Aiba, K. Katagiri, T. Kinashi, K. Nakao, et al. (2007)
Mol. Biol. Cell 18, 2949-2959
   Abstract »    Full Text »    PDF »
Targeting Protein Kinase C Activity Reporter to Discrete Intracellular Regions Reveals Spatiotemporal Differences in Agonist-dependent Signaling.
L. L. Gallegos, M. T. Kunkel, and A. C. Newton (2006)
J. Biol. Chem. 281, 30947-30956
   Abstract »    Full Text »    PDF »
Phospholipase C{epsilon} Suppresses Integrin Activation.
Y. Lad, B. McHugh, P. S. Hodkinson, A. C. MacKinnon, C. Haslett, M. H. Ginsberg, and T. Sethi (2006)
J. Biol. Chem. 281, 29501-29512
   Abstract »    Full Text »    PDF »
G{alpha}12/13- and Rho-Dependent Activation of Phospholipase C-{epsilon} by Lysophosphatidic Acid and Thrombin Receptors.
M. D. Hains, M. R. Wing, S. Maddileti, D. P. Siderovski, and T. K. Harden (2006)
Mol. Pharmacol. 69, 2068-2075
   Abstract »    Full Text »    PDF »
Hyaluronan-CD44 Interaction with Leukemia-associated RhoGEF and Epidermal Growth Factor Receptor Promotes Rho/Ras Co-activation, Phospholipase C{epsilon}-Ca2+ Signaling, and Cytoskeleton Modification in Head and Neck Squamous Cell Carcinoma Cells.
L. Y. W. Bourguignon, E. Gilad, A. Brightman, F. Diedrich, and P. Singleton (2006)
J. Biol. Chem. 281, 14026-14040
   Abstract »    Full Text »    PDF »
Ral Is both Necessary and Sufficient for the Inhibition of Myeloid Differentiation Mediated by Ras.
N. Omidvar, L. Pearn, A. K. Burnett, and R. L. Darley (2006)
Mol. Cell. Biol. 26, 3966-3975
   Abstract »    Full Text »    PDF »
The small GTPase R-Ras regulates organization of actin and drives membrane protrusions through the activity of PLC{epsilon}.
A. S. Ada-Nguema, H. Xenias, M. P. Sheetz, and P. J. Keely (2006)
J. Cell Sci. 119, 1307-1319
   Abstract »    Full Text »    PDF »
G-protein-coupled Receptor Agonists Activate Endogenous Phospholipase C{epsilon} and Phospholipase Cbeta3 in a Temporally Distinct Manner.
G. G. Kelley, K. A. Kaproth-Joslin, S. E. Reks, A. V. Smrcka, and R. J. H. Wojcikiewicz (2006)
J. Biol. Chem. 281, 2639-2648
   Abstract »    Full Text »    PDF »
Phospholipase C-{delta}1 and -{delta}3 Are Essential in the Trophoblast for Placental Development.
Y. Nakamura, Y. Hamada, T. Fujiwara, H. Enomoto, T. Hiroe, S. Tanaka, M. Nose, M. Nakahara, N. Yoshida, T. Takenawa, et al. (2005)
Mol. Cell. Biol. 25, 10979-10988
   Abstract »    Full Text »    PDF »
Phospholipase C {epsilon} Modulates {beta}-Adrenergic Receptor- Dependent Cardiac Contraction and Inhibits Cardiac Hypertrophy.
H. Wang, E. A. Oestreich, N. Maekawa, T. A. Bullard, K. L. Vikstrom, R. T. Dirksen, G. G. Kelley, B. C. Blaxall, and A. V. Smrcka (2005)
Circ. Res. 97, 1305-1313
   Abstract »    Full Text »    PDF »
A Novel Phospholipase C, PLC{eta}2, Is a Neuron-specific Isozyme.
M. Nakahara, M. Shimozawa, Y. Nakamura, Y. Irino, M. Morita, Y. Kudo, and K. Fukami (2005)
J. Biol. Chem. 280, 29128-29134
   Abstract »    Full Text »    PDF »
Regulation of Phospholipase C-{delta}1 through Direct Interactions with the Small GTPase Ral and Calmodulin.
R. S. Sidhu, R. R. Clough, and R. P. Bhullar (2005)
J. Biol. Chem. 280, 21933-21941
   Abstract »    Full Text »    PDF »
The Role of EF-hand Domains and C2 Domain in Regulation of Enzymatic Activity of Phospholipase C{zeta}.
Z. Kouchi, T. Shikano, Y. Nakamura, H. Shirakawa, K. Fukami, and S. Miyazaki (2005)
J. Biol. Chem. 280, 21015-21021
   Abstract »    Full Text »    PDF »
The Interaction of Phospholipase C-{beta}3 with Shank2 Regulates mGluR-mediated Calcium Signal.
J.-I. Hwang, H. S. Kim, J. R. Lee, E. Kim, S. H. Ryu, and P.-G. Suh (2005)
J. Biol. Chem. 280, 12467-12473
   Abstract »    Full Text »    PDF »
Congenital Semilunar Valvulogenesis Defect in Mice Deficient in Phospholipase C{varepsilon}.
M. Tadano, H. Edamatsu, S. Minamisawa, U. Yokoyama, Y. Ishikawa, N. Suzuki, H. Saito, D. Wu, M. Masago-Toda, Y. Yamawaki-Kataoka, et al. (2005)
Mol. Cell. Biol. 25, 2191-2199
   Abstract »    Full Text »    PDF »
Crucial Role of Phospholipase C{epsilon} in Chemical Carcinogen-Induced Skin Tumor Development.
Y. Bai, H. Edamatsu, S. Maeda, H. Saito, N. Suzuki, T. Satoh, and T. Kataoka (2004)
Cancer Res. 64, 8808-8810
   Abstract »    Full Text »    PDF »
The Traf2- and Nck-interacting Kinase as a Putative Effector of Rap2 to Regulate Actin Cytoskeleton.
K. Taira, M. Umikawa, K. Takei, B.-E. Myagmar, M. Shinzato, N. Machida, H. Uezato, S. Nonaka, and K.-i. Kariya (2004)
J. Biol. Chem. 279, 49488-49496
   Abstract »    Full Text »    PDF »
RhoA Activates Purified Phospholipase C-{epsilon} by a Guanine Nucleotide-dependent Mechanism.
J. P. Seifert, M. R. Wing, J. T. Snyder, S. Gershburg, J. Sondek, and T. K. Harden (2004)
J. Biol. Chem. 279, 47992-47997
   Abstract »    Full Text »    PDF »
Epac- and Ca2+-controlled Activation of Ras and Extracellular Signal-regulated Kinases by Gs-coupled Receptors.
M. Keiper, M. B. Stope, D. Szatkowski, A. Bohm, K. Tysack, F. vom Dorp, O. Saur, P. A. Oude Weernink, S. Evellin, K. H. Jakobs, et al. (2004)
J. Biol. Chem. 279, 46497-46508
   Abstract »    Full Text »    PDF »
Expression of Phospholipases A2 and C in Human Corneal Epithelial Cells.
S. Landreville, S. Coulombe, P. Carrier, M. H. Gelb, S. L. Guerin, and C. Salesse (2004)
Invest. Ophthalmol. Vis. Sci. 45, 3997-4003
   Abstract »    Full Text »    PDF »
Phospholipase C-{gamma}1 is a guanine nucleotide exchange factor for dynamin-1 and enhances dynamin-1-dependent epidermal growth factor receptor endocytosis.
J. H. Choi, J. B. Park, S. S. Bae, S. Yun, H. S. Kim, W.-P. Hong, I.-S. Kim, J. H. Kim, M. Y. Han, S. H. Ryu, et al. (2004)
J. Cell Sci. 117, 3785-3795
   Abstract »    Full Text »    PDF »
Visualizing Ras signalling in real-time.
S. A. Walker and P. J. Lockyer (2004)
J. Cell Sci. 117, 2879-2886
   Abstract »    Full Text »    PDF »
The Pleckstrin Homology Domain of Phosphoinositide-specific Phospholipase C{delta}4 Is Not a Critical Determinant of the Membrane Localization of the Enzyme.
S. B. Lee, P. Varnai, A. Balla, K. Jalink, S.-G. Rhee, and T. Balla (2004)
J. Biol. Chem. 279, 24362-24371
   Abstract »    Full Text »    PDF »
Rap2B-Dependent Stimulation of Phospholipase C-{varepsilon} by Epidermal Growth Factor Receptor Mediated by c-Src Phosphorylation of RasGRP3.
M. B. Stope, F. vom Dorp, D. Szatkowski, A. Bohm, M. Keiper, J. Nolte, P. A. Oude Weernink, D. Rosskopf, S. Evellin, K. H. Jakobs, et al. (2004)
Mol. Cell. Biol. 24, 4664-4676
   Abstract »    Full Text »    PDF »
Identification of a Novel Domain of Ras and Rap1 That Directs Their Differential Subcellular Localizations.
K. Nomura, H. Kanemura, T. Satoh, and T. Kataoka (2004)
J. Biol. Chem. 279, 22664-22673
   Abstract »    Full Text »    PDF »
Identification of Candidate Disease Genes by EST Alignments, Synteny, and Expression and Verification of Ensembl Genes on Rat Chromosome 1q43-54.
U. Vitt, D. Gietzen, K. Stevens, J. Wingrove, S. Becha, S. Bulloch, J. Burrill, N. Chawla, J. Chien, M. Crawford, et al. (2004)
Genome Res. 14, 640-650
   Abstract »    Full Text »    PDF »
Ras, Akt, and Mechanotransduction in the Cardiac Myocyte.
P. H. Sugden (2003)
Circ. Res. 93, 1179-1192
   Abstract »    Full Text »    PDF »
Direct Activation of Phospholipase C-{epsilon} by Rho.
M. R. Wing, J. T. Snyder, J. Sondek, and T. K. Harden (2003)
J. Biol. Chem. 278, 41253-41258
   Abstract »    Full Text »    PDF »
Integrated transcriptome analysis of the cellular mechanisms associated with Ha-ras-dependent malignant transformation of the human breast epithelial MCF7 cell line.
F. Gadal, C. Bozic, C. Pillot-Brochet, S. Malinge, S. Wagner, A. Le Cam, L. Buffat, M. Crepin, and F. Iris (2003)
Nucleic Acids Res. 31, 5789-5804
   Abstract »    Full Text »    PDF »
Phospholipase C-{gamma} Contains Introns Shared by src Homology 2 Domains in Many Unrelated Proteins.
C. M. Manning, W. R. Mathews, L. P. Fico, and J. R. Thackeray (2003)
Genetics 164, 433-442
   Abstract »    Full Text »    PDF »
The Direct Interaction of Phospholipase C-{gamma}1 with Phospholipase D2 Is Important for Epidermal Growth Factor Signaling.
I. H. Jang, S. Lee, J. B. Park, J. H. Kim, C. S. Lee, E.-M. Hur, I. S. Kim, K.-T. Kim, H. Yagisawa, P.-G. Suh, et al. (2003)
J. Biol. Chem. 278, 18184-18190
   Abstract »    Full Text »    PDF »
Activation of CD4 T cells by Raf-independent effectors of Ras.
J. Czyzyk, J. L. Brogdon, A. Badou, O. Henegariu, P. Preston Hurlburt, R. Flavell, and K. Bottomly (2003)
PNAS 100, 6003-6008
   Abstract »    Full Text »    PDF »
Stimulation of the ERK Pathway by GTP-loaded Rap1 Requires the Concomitant Activation of Ras, Protein Kinase C, and Protein Kinase A in Neuronal Cells.
T. Bouschet, V. Perez, C. Fernandez, J. Bockaert, A. Eychene, and L. Journot (2003)
J. Biol. Chem. 278, 4778-4785
   Abstract »    Full Text »    PDF »
Loss of Rb overrides the requirement for ERK activity for cell proliferation.
G. M. D'Abaco, S. Hooper, H. Paterson, and C. J. Marshall (2002)
J. Cell Sci. 115, 4607-4616
   Abstract »    Full Text »    PDF »
Inositol Phospholipid Metabolism in Arabidopsis. Characterized and Putative Isoforms of Inositol Phospholipid Kinase and Phosphoinositide-Specific Phospholipase C.
B. Mueller-Roeber and C. Pical (2002)
Plant Physiology 130, 22-46
   Abstract »    Full Text »    PDF »
Raf-independent Deregulation of p38 and JNK Mitogen-activated Protein Kinases Are Critical for Ras Transformation.
K. Pruitt, W. M. Pruitt, G. K. Bilter, J. K. Westwick, and C. J. Der (2002)
J. Biol. Chem. 277, 31808-31817
   Abstract »    Full Text »    PDF »
PLC{zeta}: a sperm-specific trigger of Ca2+ oscillations in eggs and embryo development.
C. M. Saunders, M. G. Larman, J. Parrington, L. J. Cox, J. Royse, L. M. Blayney, K. Swann, and F. A. Lai (2002)
Development 129, 3533-3544
   Abstract »    Full Text »    PDF »
Stimulation of Phospholipase C-{varepsilon} by the M3Muscarinic Acetylcholine Receptor Mediated by Cyclic AMP and the GTPase Rap2B.
S. Evellin, J. Nolte, K. Tysack, F. vom Dorp, M. Thiel, P. A. O. Weernink, K. H. Jakobs, E. J. Webb, J. W. Lomasney, and M. Schmidt (2002)
J. Biol. Chem. 277, 16805-16813
   Abstract »    Full Text »    PDF »
Involvement of Phosphatidylinositol 3-Kinase, but Not RalGDS, in TC21/R-Ras2-mediated Transformation.
G. A. Murphy, S. M. Graham, S. Morita, S. E. Reks, K. Rogers-Graham, A. Vojtek, G. G. Kelley, and C. J. Der (2002)
J. Biol. Chem. 277, 9966-9975
   Abstract »    Full Text »    PDF »
Absence of Erythrogenesis and Vasculogenesis in Plcg1-deficient Mice.
H.-J. Liao, T. Kume, C. McKay, M.-J. Xu, J. N. Ihle, and G. Carpenter (2002)
J. Biol. Chem. 277, 9335-9341
   Abstract »    Full Text »    PDF »
Critical Function of the Ras-associating Domain as a Primary Ras-binding Site for Regulation of Saccharomyces cerevisiae Adenylyl Cyclase.
M. Kido, F. Shima, T. Satoh, T. Asato, K.-i. Kariya, and T. Kataoka (2002)
J. Biol. Chem. 277, 3117-3123
   Abstract »    Full Text »    PDF »
Deficiency of phospholipase C-{gamma}1 impairs renal development and hematopoiesis.
M. Shirane, H. Sawa, Y. Kobayashi, T. Nakano, K. Kitajima, Y. Shinkai, K. Nagashima, and I. Negishi (2001)
Development 128, 5173-5180
   Abstract »    Full Text »    PDF »
Activation of Phospholipase C-epsilon by Heterotrimeric G Protein beta gamma -Subunits.
M. R. Wing, D. Houston, G. G. Kelley, C. J. Der, D. P. Siderovski, and T. K. Harden (2001)
J. Biol. Chem. 276, 48257-48261
   Abstract »    Full Text »    PDF »
Superantigen-Induced T Cell:B Cell Conjugation Is Mediated by LFA-1 and Requires Signaling Through Lck, But Not ZAP-70.
M. M. Morgan, C. M. Labno, G. A. Van Seventer, M. F. Denny, D. B. Straus, and J. K. Burkhardt (2001)
J. Immunol. 167, 5708-5718
   Abstract »    Full Text »    PDF »
Identification and Characterization of RA-GEF-2, a Rap Guanine Nucleotide Exchange Factor That Serves as a Downstream Target of M-Ras.
X. Gao, T. Satoh, Y. Liao, C. Song, C.-D. Hu, K.-i. Kariya, and T. Kataoka (2001)
J. Biol. Chem. 276, 42219-42225
   Abstract »    Full Text »    PDF »
A Direct Interaction between Oncogenic Ha-Ras and Phosphatidylinositol 3-Kinase Is Not Required for Ha-Ras-dependent Transformation of Epithelial Cells.
M. Karasarides, B. Anand-Apte, and A. Wolfman (2001)
J. Biol. Chem. 276, 39755-39764
   Abstract »    Full Text »    PDF »
Real Time Fluorescence Imaging of Plc{gamma} Translocation and Its Interaction with the Epidermal Growth Factor Receptor.
M. Matsuda, H. F. Paterson, R. Rodriguez, A. C. Fensome, M. V. Ellis, K. Swann, and M. Katan (2001)
J. Cell Biol. 153, 599-612
   Abstract »    Full Text »    PDF »
Phospholipase C-gamma Mediates the Hydrolysis of Phosphatidylinositol, but Not of Phosphatidylinositol 4,5-Bisphoshate, in Carbamylcholine-stimulated Islets of Langerhans.
C. J. Mitchell, M. M. Kelly, M. Blewitt, J. R. Wilson, and T. J. Biden (2001)
J. Biol. Chem. 276, 19072-19077
   Abstract »    Full Text »    PDF »
RA-GEF-1, a Guanine Nucleotide Exchange Factor for Rap1, Is Activated by Translocation Induced by Association with Rap1{middle dot}GTP and Enhances Rap1-dependent B-Raf Activation.
Y. Liao, T. Satoh, X. Gao, T.-G. Jin, C.-D. Hu, and T. Kataoka (2001)
J. Biol. Chem. 276, 28478-28483
   Abstract »    Full Text »    PDF »
Role of the CDC25 Homology Domain of Phospholipase Cepsilon in Amplification of Rap1-dependent Signaling.
T.-G. Jin, T. Satoh, Y. Liao, C. Song, X. Gao, K.-i. Kariya, C.-D. Hu, and T. Kataoka (2001)
J. Biol. Chem. 276, 30301-30307
   Abstract »    Full Text »    PDF »
Evidence for Selective Coupling of alpha 1-Adrenergic Receptors to Phospholipase C-beta 1 in Rat Neonatal Cardiomyocytes.
J. F. Arthur, S. J. Matkovich, C. J. Mitchell, T. J. Biden, and E. A. Woodcock (2001)
J. Biol. Chem. 276, 37341-37346
   Abstract »    Full Text »    PDF »

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882