Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Logo for

J. Cell Biol. 150 (6): 1311-1320

Copyright © 2000 by the Rockefeller University Press.

Original Article

Activation by Cdc42 and Pip2 of Wiskott-Aldrich Syndrome Protein (Wasp) Stimulates Actin Nucleation by Arp2/3 Complex

Henry N. Higgsa, and Thomas D. Pollarda

a The Salk Institute for Biological Studies, La Jolla, California 92037
The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037.(858) 452-3683(858) 453-4100 x1716

Abstract: We purified native WASp (Wiskott-Aldrich Syndrome protein) from bovine thymus and studied its ability to stimulate actin nucleation by Arp2/3 complex. WASp alone is inactive in the presence or absence of 0.5 µM GTP-Cdc42. Phosphatidylinositol 4,5 bisphosphate (PIP2) micelles allowed WASp to activate actin nucleation by Arp2/3 complex, and this was further enhanced twofold by GTP-Cdc42. Filaments nucleated by Arp2/3 complex and WASp in the presence of PIP2 and Cdc42 concentrated around lipid micelles and vesicles, providing that Cdc42 was GTP-bound and prenylated. Thus, the high concentration of WASp in neutrophils (9 µM) is dependent on interactions with both acidic lipids and GTP-Cdc42 to activate actin nucleation by Arp2/3 complex. The results also suggest that membrane binding increases the local concentrations of Cdc42 and WASp, favoring their interaction.

Key Words: prenylation • membrane • GBD • neutrophil • thymus

Abbreviations used in this paper: CF-PE, carboxyfluorescein-labeled PE; GBD, GTPase binding domain; GST, glutathione-S-transferase; HSS, high speed supernatant; PA, phosphatidic acid; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PIP2, phosphatidylinositol 4,5 bisphosphate; PS, phosphatidylserine; PMN, polymorphonuclear leukocytes; WA, WASp/Scar protein COOH-terminal 70–100 amino acids; WASp, Wiskott-Aldrich Syndrome protein.

NKG2D- and CD28-mediated costimulation regulate CD8+ T cell chemotaxis through different mechanisms: the role of Cdc42/N-WASp.
E. Serrano-Pertierra, E. Cernuda-Morollon, and C. Lopez-Larrea (2014)
J. Leukoc. Biol. 95, 487-495
   Abstract »    Full Text »    PDF »
Actin Monomers Activate Inverted Formin 2 by Competing with Its Autoinhibitory Interaction.
V. Ramabhadran, A. L. Hatch, and H. N. Higgs (2013)
J. Biol. Chem. 288, 26847-26855
   Abstract »    Full Text »    PDF »
Molecular mechanisms of cell-cell spread of intracellular bacterial pathogens.
K. Ireton (2013)
Open Bio 3, 130079
   Abstract »    Full Text »    PDF »
Dedicator of Cytokinesis 8 Interacts with Talin and Wiskott-Aldrich Syndrome Protein To Regulate NK Cell Cytotoxicity.
H. Ham, S. Guerrier, J. Kim, R. A. Schoon, E. L. Anderson, M. J. Hamann, Z. Lou, and D. D. Billadeau (2013)
J. Immunol. 190, 3661-3669
   Abstract »    Full Text »    PDF »
Phosphorylation of Actin-related Protein 2 (Arp2) Is Required for Normal Development and cAMP Chemotaxis in Dictyostelium.
C.-H. Choi, P. A. Thomason, M. Zaki, R. H. Insall, and D. L. Barber (2013)
J. Biol. Chem. 288, 2464-2474
   Abstract »    Full Text »    PDF »
Disease-associated missense mutations in the EVH1 domain disrupt intrinsic WASp function causing dysregulated actin dynamics and impaired dendritic cell migration.
A. J. J. Worth, J. Metelo, G. Bouma, D. Moulding, M. Fritzsche, B. Vernay, G. Charras, G. O. C. Cory, A. J. Thrasher, and S. O. Burns (2013)
Blood 121, 72-84
   Abstract »    Full Text »    PDF »
Mechanism of synergistic activation of Arp2/3 complex by cortactin and N-WASP.
L. A. Helgeson and B. J. Nolen (2013)
eLife Sci 2, e00884
   Abstract »    Full Text »    PDF »
Enteropathogenic Escherichia coli and Vaccinia Virus Do Not Require the Family of WASP-Interacting Proteins for Pathogen-Induced Actin Assembly.
J. J. Garber, F. Takeshima, I. M. Anton, M. K. Oyoshi, A. Lyubimova, A. Kapoor, T. Shibata, F. Chen, F. W. Alt, R. S. Geha, et al. (2012)
Infect. Immun. 80, 4071-4077
   Abstract »    Full Text »    PDF »
The Small GTPase RhoA Regulates the Contraction of Smooth Muscle Tissues by Catalyzing the Assembly of Cytoskeletal Signaling Complexes at Membrane Adhesion Sites.
W. Zhang, Y. Huang, and S. J. Gunst (2012)
J. Biol. Chem. 287, 33996-34008
   Abstract »    Full Text »    PDF »
Nck and Cdc42 co-operate to recruit N-WASP to promote Fc{gamma}R-mediated phagocytosis.
A. E. Dart, S. K. Donnelly, D. W. Holden, M. Way, and E. Caron (2012)
J. Cell Sci. 125, 2825-2830
   Abstract »    Full Text »    PDF »
Stoichiometry of Nck-dependent actin polymerization in living cells.
J. A. Ditlev, P. J. Michalski, G. Huber, G. M. Rivera, W. A. Mohler, L. M. Loew, and B. J. Mayer (2012)
J. Cell Biol. 197, 643-658
   Abstract »    Full Text »    PDF »
Distinct Cell Guidance Pathways Controlled by the Rac and Rho GEF Domains of UNC-73/TRIO in Caenorhabditis elegans.
N. Marcus-Gueret, K. L. Schmidt, and E. G. Stringham (2012)
Genetics 190, 129-142
   Abstract »    Full Text »    PDF »
The actin nucleation factor JMY is a negative regulator of neuritogenesis.
E. N. Firat-Karalar, P. P. Hsiue, and M. D. Welch (2011)
Mol. Biol. Cell 22, 4563-4574
   Abstract »    Full Text »    PDF »
A Novel Phospholipase D2-Grb2-WASp Heterotrimer Regulates Leukocyte Phagocytosis in a Two-Step Mechanism.
S. Kantonen, N. Hatton, M. Mahankali, K. M. Henkels, H. Park, D. Cox, and J. Gomez-Cambronero (2011)
Mol. Cell. Biol. 31, 4524-4537
   Abstract »    Full Text »    PDF »
Host-pathogen interactions: Pathogens and polymers: Microbe-host interactions illuminate the cytoskeleton.
C. M. Haglund and M. D. Welch (2011)
J. Cell Biol. 195, 7-17
   Abstract »    Full Text »    PDF »
Structural and biochemical characterization of two binding sites for nucleation-promoting factor WASp-VCA on Arp2/3 complex.
S.-C. Ti, C. T. Jurgenson, B. J. Nolen, and T. D. Pollard (2011)
PNAS 108, E463-E471
   Abstract »    Full Text »    PDF »
Identification of Fyn as the binding partner for the WASP N-terminal domain in T cells.
M. Sato, R. Sawahata, T. Takenouchi, and H. Kitani (2011)
Int. Immunol. 23, 493-502
   Abstract »    Full Text »    PDF »
Mechanism of a Concentration-dependent Switch between Activation and Inhibition of Arp2/3 Complex by Coronin.
S.-L. Liu, K. M. Needham, J. R. May, and B. J. Nolen (2011)
J. Biol. Chem. 286, 17039-17046
   Abstract »    Full Text »    PDF »
WASP is activated by phosphatidylinositol-4,5-bisphosphate to restrict synapse growth in a pathway parallel to bone morphogenetic protein signaling.
T. M. Khuong, R. L. P. Habets, J. R. Slabbaert, and P. Verstreken (2010)
PNAS 107, 17379-17384
   Abstract »    Full Text »    PDF »
c-mip Impairs Podocyte Proximal Signaling and Induces Heavy Proteinuria.
S. y. Zhang, M. Kamal, K. Dahan, A. Pawlak, V. Ory, D. Desvaux, V. Audard, M. Candelier, F. BenMohamed, M. Matignon, et al. (2010)
Science Signaling 3, ra39
   Abstract »    Full Text »    PDF »
Regulation of the Actin Cytoskeleton-Plasma Membrane Interplay by Phosphoinositides.
J. Saarikangas, H. Zhao, and P. Lappalainen (2010)
Physiol Rev 90, 259-289
   Abstract »    Full Text »    PDF »
Platelet-associated IgAs and impaired GPVI responses in platelets lacking WIP.
H. Falet, M. P. Marchetti, K. M. Hoffmeister, M. J. Massaad, R. S. Geha, and J. H. Hartwig (2009)
Blood 114, 4729-4737
   Abstract »    Full Text »    PDF »
Regulation of podosome dynamics by WASp phosphorylation: implication in matrix degradation and chemotaxis in macrophages.
A. Dovas, J.-C. Gevrey, A. Grossi, H. Park, W. Abou-Kheir, and D. Cox (2009)
J. Cell Sci. 122, 3873-3882
   Abstract »    Full Text »    PDF »
Phosphorylation of WASp is a key regulator of activity and stability in vivo.
M. P. Blundell, G. Bouma, J. Metelo, A. Worth, Y. Calle, L. A. Cowell, L. S. Westerberg, D. A. Moulding, S. Mirando, C. Kinnon, et al. (2009)
PNAS 106, 15738-15743
   Abstract »    Full Text »    PDF »
A Phosphatidylinositol-Transfer Protein and Phosphatidylinositol-4-phosphate 5-Kinase Control Cdc42 to Regulate the Actin Cytoskeleton and Secretory Pathway in Yeast.
L. Yakir-Tamang and J. E. Gerst (2009)
Mol. Biol. Cell 20, 3583-3597
   Abstract »    Full Text »    PDF »
Leukocyte trafficking in primary immunodeficiencies.
L. D. Notarangelo and R. Badolato (2009)
J. Leukoc. Biol. 85, 335-343
   Abstract »    Full Text »    PDF »
The cell migration molecule UNC-53/NAV2 is linked to the ARP2/3 complex by ABI-1.
K. L. Schmidt, N. Marcus-Gueret, A. Adeleye, J. Webber, D. Baillie, and E. G. Stringham (2009)
Development 136, 563-574
   Abstract »    Full Text »    PDF »
Grab Your Partner with Both Hands: Cytoskeletal Remodeling by Arp2/3 Signaling.
S. H. Soderling (2009)
Science Signaling 2, pe5
   Abstract »    Full Text »    PDF »
Kank attenuates actin remodeling by preventing interaction between IRSp53 and Rac1.
B. Chandra Roy, N. Kakinuma, and R. Kiyama (2009)
J. Cell Biol. 184, 253-267
   Abstract »    Full Text »    PDF »
Btk Regulates B Cell Receptor-Mediated Antigen Processing and Presentation by Controlling Actin Cytoskeleton Dynamics in B Cells.
S. Sharma, G. Orlowski, and W. Song (2009)
J. Immunol. 182, 329-339
   Abstract »    Full Text »    PDF »
Regulation of WAVE1 expression in macrophages at multiple levels.
H. Dinh, G. M. Scholz, and J. A. Hamilton (2008)
J. Leukoc. Biol. 84, 1483-1491
   Abstract »    Full Text »    PDF »
Close Packing of Listeria monocytogenes ActA, a Natively Unfolded Protein, Enhances F-actin Assembly without Dimerization.
M. J. Footer, J. K. Lyo, and J. A. Theriot (2008)
J. Biol. Chem. 283, 23852-23862
   Abstract »    Full Text »    PDF »
Nervous Wreck and Cdc42 Cooperate to Regulate Endocytic Actin Assembly during Synaptic Growth.
A. A. Rodal, R. N. Motola-Barnes, and J. T. Littleton (2008)
J. Neurosci. 28, 8316-8325
   Abstract »    Full Text »    PDF »
Physiologic Properties and Regulation of the Actin Cytoskeleton in Vascular Smooth Muscle.
D. D. Tang and Y. Anfinogenova (2008)
Journal of Cardiovascular Pharmacology and Therapeutics 13, 130-140
   Abstract »    PDF »
Wiskott-Aldrich Syndrome Protein Is a Key Regulator of the Phagocytic Cup Formation in Macrophages.
S. Tsuboi and J. Meerloo (2007)
J. Biol. Chem. 282, 34194-34203
   Abstract »    Full Text »    PDF »
Stimulation of Actin Polymerization by Vacuoles via Cdc42p-dependent Signaling.
S. Isgandarova, L. Jones, D. Forsberg, A. Loncar, J. Dawson, K. Tedrick, and G. Eitzen (2007)
J. Biol. Chem. 282, 30466-30475
   Abstract »    Full Text »    PDF »
Unregulated actin polymerization by WASp causes defects of mitosis and cytokinesis in X-linked neutropenia.
D. A. Moulding, M. P. Blundell, D. G. Spiller, M. R.H. White, G. O. Cory, Y. Calle, H. Kempski, J. Sinclair, P. J. Ancliff, C. Kinnon, et al. (2007)
J. Exp. Med. 204, 2213-2224
   Abstract »    Full Text »    PDF »
Filopodia: The Fingers That Do the Walking.
S. L. Gupton and F. B. Gertler (2007)
Sci. STKE 2007, re5
   Abstract »    Full Text »    PDF »
A Conserved Family of Enzymes That Phosphorylate Inositol Hexakisphosphate.
S. Mulugu, W. Bai, P. C. Fridy, R. J. Bastidas, J. C. Otto, D. E. Dollins, T. A. Haystead, A. A. Ribeiro, and J. D. York (2007)
Science 316, 106-109
   Abstract »    Full Text »    PDF »
Multiple WASP-interacting Protein Recognition Motifs Are Required for a Functional Interaction with N-WASP.
F. C. Peterson, Q. Deng, M. Zettl, K. E. Prehoda, W. A. Lim, M. Way, and B. F. Volkman (2007)
J. Biol. Chem. 282, 8446-8453
   Abstract »    Full Text »    PDF »
WASP regulates suppressor activity of human and murine CD4+CD25+FOXP3+ natural regulatory T cells.
F. Marangoni, S. Trifari, S. Scaramuzza, C. Panaroni, S. Martino, L. D. Notarangelo, Z. Baz, A. Metin, F. Cattaneo, A. Villa, et al. (2007)
J. Exp. Med. 204, 369-380
   Abstract »    Full Text »    PDF »
N-WASP Involvement in Dorsal Ruffle Formation in Mouse Embryonic Fibroblasts.
J. A. Legg, G. Bompard, J. Dawson, H. L. Morris, N. Andrew, L. Cooper, S. A. Johnston, G. Tramountanis, and L. M. Machesky (2007)
Mol. Biol. Cell 18, 678-687
   Abstract »    Full Text »    PDF »
Visualizing Arp2/3 complex activation mediated by binding of ATP and WASp using structural mass spectrometry.
J. G. Kiselar, R. Mahaffy, T. D. Pollard, S. C. Almo, and M. R. Chance (2007)
PNAS 104, 1552-1557
   Abstract »    Full Text »    PDF »
Hyaluronan-CD44 Interaction with Neural Wiskott-Aldrich Syndrome Protein (N-WASP) Promotes Actin Polymerization and ErbB2 Activation Leading to beta-Catenin Nuclear Translocation, Transcriptional Up-regulation, and Cell Migration in Ovarian Tumor Cells.
L. Y. W. Bourguignon, K. Peyrollier, E. Gilad, and A. Brightman (2007)
J. Biol. Chem. 282, 1265-1280
   Abstract »    Full Text »    PDF »
Two novel activating mutations in the Wiskott-Aldrich syndrome protein result in congenital neutropenia.
P. J. Ancliff, M. P. Blundell, G. O. Cory, Y. Calle, A. Worth, H. Kempski, S. Burns, G. E. Jones, J. Sinclair, C. Kinnon, et al. (2006)
Blood 108, 2182-2189
   Abstract »    Full Text »    PDF »
Myosin-X is a molecular motor that functions in filopodia formation.
A. B. Bohil, B. W. Robertson, and R. E. Cheney (2006)
PNAS 103, 12411-12416
   Abstract »    Full Text »    PDF »
Tuba stimulates intracellular N-WASP-dependent actin assembly.
E. M. Kovacs, R. S. Makar, and F. B. Gertler (2006)
J. Cell Sci. 119, 2715-2726
   Abstract »    Full Text »    PDF »
A Complex of Wiskott-Aldrich Syndrome Protein with Mammalian Verprolins Plays an Important Role in Monocyte Chemotaxis.
S. Tsuboi (2006)
J. Immunol. 176, 6576-6585
   Abstract »    Full Text »    PDF »
WAVE1 Is Required for Oligodendrocyte Morphogenesis and Normal CNS Myelination.
H.-J. Kim, A. B. DiBernardo, J. A. Sloane, M. N. Rasband, D. Solomon, B. Kosaras, S. P. Kwak, and T. K. Vartanian (2006)
J. Neurosci. 26, 5849-5859
   Abstract »    Full Text »    PDF »
Formation of a WIP-, WASp-, actin-, and myosin IIA-containing multiprotein complex in activated NK cells and its alteration by KIR inhibitory signaling..
K. Krzewski, X. Chen, J. S. Orange, and J. L. Strominger (2006)
J. Cell Biol. 173, 121-132
   Abstract »    Full Text »    PDF »
Phosphorylation of WASP by the Cdc42-associated Kinase ACK1: DUAL HYDROXYAMINO ACID SPECIFICITY IN A TYROSINE KINASE.
N. Yokoyama, J. Lougheed, and W. T. Miller (2005)
J. Biol. Chem. 280, 42219-42226
   Abstract »    Full Text »    PDF »
Counting Cytokinesis Proteins Globally and Locally in Fission Yeast.
J.-Q. Wu and T. D. Pollard (2005)
Science 310, 310-314
   Abstract »    Full Text »    PDF »
Role of the WASP family proteins for Mycobacterium marinum actin tail formation.
L. M. Stamm, M. A. Pak, J. H. Morisaki, S. B. Snapper, K. Rottner, S. Lommel, and E. J. Brown (2005)
PNAS 102, 14837-14842
   Abstract »    Full Text »    PDF »
Lipid rafts and regulation of the cytoskeleton during T cell activation.
K. F. Meiri (2005)
Phil Trans R Soc B 360, 1663-1672
   Abstract »    Full Text »    PDF »
Regulation of Actin Ring Formation by Rho GTPases in Osteoclasts.
M. A. Chellaiah (2005)
J. Biol. Chem. 280, 32930-32943
   Abstract »    Full Text »    PDF »
WAVE2 Signaling Mediates Invasion of Polarized Epithelial Cells by Salmonella typhimurium.
J. Shi, G. Scita, and J. E. Casanova (2005)
J. Biol. Chem. 280, 29849-29855
   Abstract »    Full Text »    PDF »
The Adapter Protein CrkII Regulates Neuronal Wiskott-Aldrich Syndrome Protein, Actin Polymerization, and Tension Development during Contractile Stimulation of Smooth Muscle.
D. D. Tang, W. Zhang, and S. J. Gunst (2005)
J. Biol. Chem. 280, 23380-23389
   Abstract »    Full Text »    PDF »
WAVE3-mediated Cell Migration and Lamellipodia Formation Are Regulated Downstream of Phosphatidylinositol 3-Kinase.
K. Sossey-Alaoui, X. Li, T. A. Ranalli, and J. K. Cowell (2005)
J. Biol. Chem. 280, 21748-21755
   Abstract »    Full Text »    PDF »
Activation of the Arp2/3 complex by N-WASp is required for actin polymerization and contraction in smooth muscle.
W. Zhang, Y. Wu, L. Du, D. D. Tang, and S. J. Gunst (2005)
Am J Physiol Cell Physiol 288, C1145-C1160
   Abstract »    Full Text »    PDF »
A Dictyostelium Homologue of WASP Is Required for Polarized F-Actin Assembly during Chemotaxis.
S. A. Myers, J. W. Han, Y. Lee, R. A. Firtel, and C. Y. Chung (2005)
Mol. Biol. Cell 16, 2191-2206
   Abstract »    Full Text »    PDF »
Spatially Distinct Binding of Cdc42 to PAK1 and N-WASP in Breast Carcinoma Cells.
M. Parsons, J. Monypenny, S. M. Ameer-Beg, T. H. Millard, L. M. Machesky, M. Peter, M. D. Keppler, G. Schiavo, R. Watson, J. Chernoff, et al. (2005)
Mol. Cell. Biol. 25, 1680-1695
   Abstract »    Full Text »    PDF »
The Wiskott-Aldrich Syndrome Protein Regulates Nuclear Translocation of NFAT2 and NF-{kappa}B (RelA) Independently of Its Role in Filamentous Actin Polymerization and Actin Cytoskeletal Rearrangement.
W. Huang, H. D. Ochs, B. Dupont, and Y. M. Vyas (2005)
J. Immunol. 174, 2602-2611
   Abstract »    Full Text »    PDF »
Cortactin regulates cell migration through activation of N-WASP.
J. R. Kowalski, C. Egile, S. Gil, S. B. Snapper, R. Li, and S. M. Thomas (2005)
J. Cell Sci. 118, 79-87
   Abstract »    Full Text »    PDF »
Interdependent assembly of specific regulatory lipids and membrane fusion proteins into the vertex ring domain of docked vacuoles.
R. A. Fratti, Y. Jun, A. J. Merz, N. Margolis, and W. Wickner (2004)
J. Cell Biol. 167, 1087-1098
   Abstract »    Full Text »    PDF »
Diacylglycerol and Its Formation by Phospholipase C Regulate Rab- and SNARE-dependent Yeast Vacuole Fusion.
Y. Jun, R. A. Fratti, and W. Wickner (2004)
J. Biol. Chem. 279, 53186-53195
   Abstract »    Full Text »    PDF »
The Small GTPase Cdc42 Regulates Actin Polymerization and Tension Development during Contractile Stimulation of Smooth Muscle.
D. D. Tang and S. J. Gunst (2004)
J. Biol. Chem. 279, 51722-51728
   Abstract »    Full Text »    PDF »
Mechanisms of WASp-mediated hematologic and immunologic disease.
S. Burns, G. O. Cory, W. Vainchenker, and A. J. Thrasher (2004)
Blood 104, 3454-3462
   Abstract »    Full Text »    PDF »
Crystal structures of actin-related protein 2/3 complex with bound ATP or ADP.
B. J. Nolen, R. S. Littlefield, and T. D. Pollard (2004)
PNAS 101, 15627-15632
   Abstract »    Full Text »    PDF »
Na+/H+ exchanger NHE1 as plasma membrane scaffold in the assembly of signaling complexes.
M. Baumgartner, H. Patel, and D. L. Barber (2004)
Am J Physiol Cell Physiol 287, C844-C850
   Abstract »    Full Text »    PDF »
Enhanced Membrane Fusion in Sterol-enriched Vacuoles Bypasses the Vrp1p Requirement.
K. Tedrick, T. Trischuk, R. Lehner, and G. Eitzen (2004)
Mol. Biol. Cell 15, 4609-4621
   Abstract »    Full Text »    PDF »
Activation of Endogenous Cdc42 Visualized in Living Cells.
P. Nalbant, L. Hodgson, V. Kraynov, A. Toutchkine, and K. M. Hahn (2004)
Science 305, 1615-1619
   Abstract »    Full Text »    PDF »
Erk/Src Phosphorylation of Cortactin Acts as a Switch On-Switch Off Mechanism That Controls Its Ability To Activate N-WASP.
N. Martinez-Quiles, H.-Y. H. Ho, M. W. Kirschner, N. Ramesh, and R. S. Geha (2004)
Mol. Cell. Biol. 24, 5269-5280
   Abstract »    Full Text »    PDF »
The Mouse Formin, FRL{alpha}, Slows Actin Filament Barbed End Elongation, Competes with Capping Protein, Accelerates Polymerization from Monomers, and Severs Filaments.
E. S. Harris, F. Li, and H. N. Higgs (2004)
J. Biol. Chem. 279, 20076-20087
   Abstract »    Full Text »    PDF »
Cascade pathway of filopodia formation downstream of SCAR.
A. Biyasheva, T. Svitkina, P. Kunda, B. Baum, and G. Borisy (2004)
J. Cell Sci. 117, 837-848
   Abstract »    Full Text »    PDF »
Visualization of spatially and temporally regulated N-WASP activity during cytoskeletal reorganization in living cells.
M. E. Ward, J. Y. Wu, and Y. Rao (2004)
PNAS 101, 970-974
   Abstract »    Full Text »    PDF »
Tuba, a Novel Protein Containing Bin/Amphiphysin/Rvs and Dbl Homology Domains, Links Dynamin to Regulation of the Actin Cytoskeleton.
M. A. Salazar, A. V. Kwiatkowski, L. Pellegrini, G. Cestra, M. H. Butler, K. L. Rossman, D. M. Serna, J. Sondek, F. B. Gertler, and P. De Camilli (2003)
J. Biol. Chem. 278, 49031-49043
   Abstract »    Full Text »    PDF »
Translocation of N-WASP by Nuclear Localization and Export Signals into the Nucleus Modulates Expression of HSP90.
S. Suetsugu and T. Takenawa (2003)
J. Biol. Chem. 278, 42515-42523
   Abstract »    Full Text »    PDF »
Downregulation of profilin with antisense oligodeoxynucleotides inhibits force development during stimulation of smooth muscle.
D. D. Tang and J. Tan (2003)
Am J Physiol Heart Circ Physiol 285, H1528-H1536
   Abstract »    Full Text »    PDF »
Role of Crk-Associated Substrate in the Regulation of Vascular Smooth Muscle Contraction.
D. D. Tang and J. Tan (2003)
Hypertension 42, 858-863
   Abstract »    Full Text »    PDF »
Regulation of Actin Dynamics by WASP Family Proteins.
H. Miki and T. Takenawa (2003)
J. Biochem. 134, 309-313
   Abstract »    Full Text »    PDF »
Arf6 and Phosphoinositol-4-Phosphate-5-Kinase Activities Permit Bypass of the Rac1 Requirement for {beta}1 Integrin-mediated Bacterial Uptake.
K.-W. Wong and R. R. Isberg (2003)
J. Exp. Med. 198, 603-614
   Abstract »    Full Text »    PDF »
A defect in hematopoietic stem cell migration explains the nonrandom X-chromosome inactivation in carriers of Wiskott-Aldrich syndrome.
C. Lacout, E. Haddad, S. Sabri, F. Svinarchouk, L. Garcon, C. Capron, A. Foudi, R. Mzali, S. B. Snapper, F. Louache, et al. (2003)
Blood 102, 1282-1289
   Abstract »    Full Text »    PDF »
Sequential Interaction of Actin-related Proteins 2 and 3 (Arp2/3) Complex with Neural Wiscott-Aldrich Syndrome Protein (N-WASP) and Cortactin during Branched Actin Filament Network Formation.
T. Uruno, J. Liu, Y. Li, N. Smith, and X. Zhan (2003)
J. Biol. Chem. 278, 26086-26093
   Abstract »    Full Text »    PDF »
Membrane Ruffling Requires Coordination between Type I{alpha} Phosphatidylinositol Phosphate Kinase and Rac Signaling.
R. L. Doughman, A. J. Firestone, M. L. Wojtasiak, M. W. Bunce, and R. A. Anderson (2003)
J. Biol. Chem. 278, 23036-23045
   Abstract »    Full Text »    PDF »
Felic (CIP4b), a novel binding partner with the Src kinase Lyn and Cdc42, localizes to the phagocytic cup.
P. Dombrosky-Ferlan, A. Grishin, R. J. Botelho, M. Sampson, L. Wang, W. A. Rudert, S. Grinstein, and S. J. Corey (2003)
Blood 101, 2804-2809
   Abstract »    Full Text »    PDF »
Mouse MIM, a Tissue-specific Regulator of Cytoskeletal Dynamics, Interacts with ATP-Actin Monomers through Its C-terminal WH2 Domain.
P. K. Mattila, M. Salminen, T. Yamashiro, and P. Lappalainen (2003)
J. Biol. Chem. 278, 8452-8459
   Abstract »    Full Text »    PDF »
EPLIN regulates actin dynamics by cross-linking and stabilizing filaments.
R. S. Maul, Y. Song, K. J. Amann, S. C. Gerbin, T. D. Pollard, and D. D. Chang (2003)
J. Cell Biol. 160, 399-407
   Abstract »    Full Text »    PDF »
Signal transduction during Fc receptor-mediated phagocytosis.
E. Garcia-Garcia and C. Rosales (2002)
J. Leukoc. Biol. 72, 1092-1108
   Abstract »    Full Text »    PDF »
Phosphorylation of Tyrosine 291 Enhances the Ability of WASp to Stimulate Actin Polymerization and Filopodium Formation.
G. O. C. Cory, R. Garg, R. Cramer, and A. J. Ridley (2002)
J. Biol. Chem. 277, 45115-45121
   Abstract »    Full Text »    PDF »
Motility Determinants in WASP Family Proteins.
D. Yarar, J. A. D'Alessio, R. L. Jeng, and M. D. Welch (2002)
Mol. Biol. Cell 13, 4045-4059
   Abstract »    Full Text »    PDF »
Lateral Sequestration of Phosphatidylinositol 4,5-Bisphosphate by the Basic Effector Domain of Myristoylated Alanine-rich C Kinase Substrate Is Due to Nonspecific Electrostatic Interactions.
J. Wang, A. Gambhir, G. Hangyas-Mihalyne, D. Murray, U. Golebiewska, and S. McLaughlin (2002)
J. Biol. Chem. 277, 34401-34412
   Abstract »    Full Text »    PDF »
Remodeling of organelle-bound actin is required for yeast vacuole fusion.
G. Eitzen, L. Wang, N. Thorngren, and W. Wickner (2002)
J. Cell Biol. 158, 669-679
   Abstract »    Full Text »    PDF »
Normal Arp2/3 complex activation in platelets lacking WASp.
H. Falet, K. M. Hoffmeister, R. Neujahr, and J. H. Hartwig (2002)
Blood 100, 2113-2122
   Abstract »    Full Text »    PDF »

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882