Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Logo for

Mol. Cell. Biol. 22 (6): 1792-1803

Copyright © 2002 by the American Society for Microbiology. All rights reserved.

CKA, a Novel Multidomain Protein, Regulates the JUN N-Terminal Kinase Signal Transduction Pathway in Drosophila

Hua-Wei Chen,1 Maria Julia Marinissen,2 Su-Wan Oh,1 Xiu Chen,1 Michael Melnick,3 Norbert Perrimon,4 J. Silvio Gutkind,2 and Steven X. Hou1*

The Laboratory of Immunobiology, National Institutes of Health, National Cancer Institute at Frederick, Frederick, Maryland 21702,1 Laboratory of Cell Signaling, New England BioLabs, Beverly, Massachusetts 01915,3 Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892,2 Howard Hughes Medical Institute, Department of Genetics, Harvard Medical School, Boston, Massachusetts 021154

Received for publication 31 July 2001. Revision received 18 September 2001. Accepted for publication 4 December 2001.

Abstract: The Drosophila melanogaster JUN N-terminal kinase (DJNK) and DPP (decapentaplegic) signal transduction pathways coordinately regulate epithelial cell sheet movement during the process of dorsal closure in the embryo. By a genetic screen of mutations affecting dorsal closure in Drosophila, we have now identified a multidomain protein, connector of kinase to AP-1 (cka), that functions in the DJNK pathway and controls the localized expression of dpp in the leading-edge cells. We have also investigated how CKA acts. This unique molecule forms a complex with HEP (DJNKK), BSK (DJNK), DJUN, and DFOS. Complex formation activates BSK kinase, which in turn phosphorylates and activates DJUN and DFOS. These data suggest that CKA represents a novel molecule regulating AP-1 activity by organizing a molecular complex of kinases and transcription factors, thus coordinating the spatial-temporal expression of AP-1-regulated genes.


* Corresponding author. Mailing address: Building 560, Room 12-70, NCI-FCRDC, National Cancer Institute at Frederick, Frederick, MD 21702. Phone: (301) 846-1589. Fax: (301) 846-6145. E-mail: shou{at}mail.ncifcrf.gov.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Interactions between the amnioserosa and the epidermis revealed by the function of the u-shaped gene.
K. Lada, N. Gorfinkiel, and A. Martinez Arias (2012)
Biology Open 1, 353-361
   Abstract »    Full Text »    PDF »
Drosophila Smt3 negatively regulates JNK signaling through sequestering Hipk in the nucleus.
H. Huang, G. Du, H. Chen, X. Liang, C. Li, N. Zhu, L. Xue, J. Ma, and R. Jiao (2011)
Development 138, 2477-2485
   Abstract »    Full Text »    PDF »
The coiled-coil protein-binding motif in Fusarium verticillioides Fsr1 is essential for maize stalk rot virulence.
Y. Yamamura and W.-B. Shim (2008)
Microbiology 154, 1637-1645
   Abstract »    Full Text »    PDF »
A WD40 Repeat Protein Regulates Fungal Cell Differentiation and Can Be Replaced Functionally by the Mammalian Homologue Striatin.
S. Poggeler and U. Kuck (2004)
Eukaryot. Cell 3, 232-240
   Abstract »    Full Text »    PDF »
bullwinkle and shark regulate dorsal-appendage morphogenesis in Drosophila oogenesis.
D. H. Tran and C. A. Berg (2003)
Development 130, 6273-6282
   Abstract »    Full Text »    PDF »

To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882