Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Logo for

Mol. Biol. Cell 12 (2): 383-392

Copyright © 2001 by The American Society for Cell Biology.

Vol. 12, Issue 2, 383-392, February 2001

The Cellular Level of PR500, a Protein Complex Related to the 19S Regulatory Particle of the Proteasome, Is Regulated in Response to Stresses in Plants

Zhaohua Peng,* Jeffrey M. Staub,*dagger Giovanna Serino,* Shing F. Kwok,*Dagger Jasmina Kurepa,§ Barry D. Bruce,|| Richard D. Vierstra,§ Ning Wei,* and Xing-Wang Deng*

 *Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520-8104;  ||Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee at Knoxville, Knoxville, Tennessee 37996; and  §Cellular and Molecular Biology Program, University of Wisconsin-Madison, Madison, Wisconsin 53706

In Arabidopsis seedlings and cauliflower florets, Rpn6 (a proteasome non-ATPase regulatory subunit) was found in two distinct protein complexes of ~800 and 500 kDa, respectively. The large complex likely represents the proteasome 19S regulator particle (RP) because it displays the expected subunit composition and all characteristics. The small complex, designated PR500, shares at least three subunits with the "lid" subcomplex of 19S RP and is loosely associated with an hsp70 protein. In Arabidopsis COP9 signalosome mutants, PR500 was specifically absent or reduced to an extent that correlates with the severity of the mutations. Furthermore, PR500 was also diminished in response to potential protein-misfolding stresses caused by the heat shock and canavanine treatment. Immunofluorescence studies suggest that PR500 has a distinct localization pattern and is enriched in specific nuclear foci. We propose that PR500 may be evolved in higher plants to cope with the frequently encountered environmental stresses.


Present addresses: dagger Monsanto Company, 700 Chesterfield Parkway North, Mail Code BB3G, St. Louis, MO 63017; Dagger Ceres, Inc., 3007 Malibu Canyon Road, Malibu, CA 90265.

Corresponding author. E-mail address: xingwang.deng{at}yale.edu.


Molecular Biology of the Cell
Vol. 12, 383-392, February 2001
Copyright © 2001 by The American Society for Cell Biology

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Dynamic Regulation of the COP9 Signalosome in Response to DNA Damage.
M. G. Fuzesi-Levi, G. Ben-Nissan, E. Bianchi, H. Zhou, M. J. Deery, K. S. Lilley, Y. Levin, and M. Sharon (2014)
Mol. Cell. Biol. 34, 1066-1076
   Abstract »    Full Text »    PDF »
The Arabidopsis COP9 SIGNALOSOME INTERACTING F-BOX KELCH 1 Protein Forms an SCF Ubiquitin Ligase and Regulates Hypocotyl Elongation.
A. Franciosini, B. Lombardi, S. Iafrate, V. Pecce, G. Mele, L. Lupacchini, G. Rinaldi, Y. Kondou, G. Gusmaroli, S. Aki, et al. (2013)
Mol Plant 6, 1616-1629
   Abstract »    Full Text »    PDF »
Malformed Selenoproteins Are Removed by the Ubiquitin-Proteasome Pathway in Stanleya pinnata.
M. Sabbagh and D. Van Hoewyk (2012)
Plant Cell Physiol. 53, 555-564
   Abstract »    Full Text »    PDF »
The 26S Proteasome Function and Hsp90 Activity Involved in the Regulation of HsfA2 Expression in Response to Oxidative Stress.
A. Nishizawa-Yokoi, H. Tainaka, E. Yoshida, M. Tamoi, Y. Yabuta, and S. Shigeoka (2010)
Plant Cell Physiol. 51, 486-496
   Abstract »    Full Text »    PDF »
Arabidopsis PUB22 and PUB23 Are Homologous U-Box E3 Ubiquitin Ligases That Play Combinatory Roles in Response to Drought Stress.
S. K. Cho, M. Y. Ryu, C. Song, J. M. Kwak, and W. T. Kim (2008)
PLANT CELL 20, 1899-1914
   Abstract »    Full Text »    PDF »
Characterization of the VIER F-BOX PROTEINE Genes from Arabidopsis Reveals Their Importance for Plant Growth and Development.
K. M. Schwager, L. I. A. Calderon-Villalobos, E. M.N. Dohmann, B. C. Willige, S. Knierer, C. Nill, and C. Schwechheimer (2007)
PLANT CELL 19, 1163-1178
   Abstract »    Full Text »    PDF »
Heterologous Expression and Molecular and Cellular Characterization of CaPUB1 Encoding a Hot Pepper U-Box E3 Ubiquitin Ligase Homolog.
S. K. Cho, H. S. Chung, M. Y. Ryu, M. J. Park, M. M. Lee, Y.-Y. Bahk, J. Kim, H. S. Pai, and W. T. Kim (2006)
Plant Physiology 142, 1664-1682
   Abstract »    Full Text »    PDF »
Rpn6p, a Proteasome Subunit from Saccharomyces cerevisiae, Is Essential for the Assembly and Activity of the 26 S Proteasome.
P. G. Santamaria, D. Finley, J. P. G. Ballesta, and M. Remacha (2003)
J. Biol. Chem. 278, 6687-6695
   Abstract »    Full Text »    PDF »
Missense Mutation in the PAS2 Domain of Phytochrome A Impairs Subnuclear Localization and a Subset of Responses.
M. J. Yanovsky, J. P. Luppi, D. Kirchbauer, O. B. Ogorodnikova, V. A. Sineshchekov, E. Adam, S. Kircher, R. J. Staneloni, E. Schafer, F. Nagy, et al. (2002)
PLANT CELL 14, 1591-1603
   Abstract »    Full Text »    PDF »
Cytokinin Growth Responses in Arabidopsis Involve the 26S Proteasome Subunit RPN12.
J. Smalle, J. Kurepa, P. Yang, E. Babiychuk, S. Kushnir, A. Durski, and R. D. Vierstra (2002)
PLANT CELL 14, 17-32
   Abstract »    Full Text »    PDF »
Subunit interaction maps for the regulatory particle of the 26S proteasome and the COP9 signalosome.
H. Fu, N. Reis, Y. Lee, M. H. Glickman, and R. D. Vierstra (2001)
EMBO J. 20, 7096-7107
   Abstract »    Full Text »    PDF »
Molecular Characterization of Subunit 6 of the COP9 Signalosome and Its Role in Multifaceted Developmental Processes in Arabidopsis.
Z. Peng, G. Serino, and X.-W. Deng (2001)
PLANT CELL 13, 2393-2407
   Abstract »    Full Text »    PDF »
LAF1, a MYB transcription activator for phytochrome A signaling.
M. L. Ballesteros, C. Bolle, L. M. Lois, J. M. Moore, J.-P. Vielle-Calzada, U. Grossniklaus, and N.-H. Chua (2001)
Genes & Dev. 15, 2613-2625
   Abstract »    Full Text »    PDF »
A Hitchhiker's Guide to the Proteasome.
A. G. von Arnim (2001)
Sci. STKE 2001, pe2
   Abstract »    Full Text »    PDF »
Saccharomyces cerevisiae Protein Pci8p and Human Protein eIF3e/Int-6 Interact with the eIF3 Core Complex by Binding to Cognate eIF3b Subunits.
A. Shalev, L. Valasek, C. A. Pise-Masison, M. Radonovich, L. Phan, J. Clayton, H. He, J. N. Brady, A. G. Hinnebusch, and K. Asano (2001)
J. Biol. Chem. 276, 34948-34957
   Abstract »    Full Text »    PDF »

To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882