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PLANT CELL 12 (9): 1679-1688

Copyright © 2000 by the American Society of Plant Physiologists.

Plant Cell, Vol. 12, 1679-1688, September 2000, Copyright © 2000, American Society of Plant Physiologists Association of Phosphatidylinositol 3-Kinase with Nuclear Transcription Sites in Higher Plants Tom D. Bunneya, Peter A. C. Watkinsa, Alison F. Bevena, Peter J. Shawa, Luis E. Hernandeza, George P. Lomonossoffa, Mike Shanksa, Jan Pearta, and Bjørn K. Drøbaka a John Innes Centre, Norwich Research Park, Colney, NR4 7UH, United Kingdom Bjørn K. Drøbak, bjorn.drobak{at} (E-mail), 44-0-1603-501771 (fax)

The kinases responsible for phosphorylation of inositol-containing lipids are essential for many aspects of normal eukaryotic cell function. Genetic and biochemical studies have established that the phosphatidylinositol (PtdIns) 3-kinase encoded by the yeast VPS34 gene is essential for the efficient sorting and delivery of proteins to the vacuole; the kinase encoded by the human VPS34 homolog has been equally implicated in the control of intracellular vesicle traffic. The plant VPS34 homolog also is required for normal growth and development, and although a role for PtdIns 3-kinase in vesicle trafficking is likely, it has not been established. In this study, we have shown that considerable PtdIns 3-kinase activity is associated with the internal matrix of nuclei isolated from carrot suspension cells. Immunocytochemical and confocal laser scanning microscopy studies using the monoclonal antibody JIM135 (John Innes Monoclonal 135), raised against a truncated version of the soybean PtdIns 3-kinase, SPI3K-5p, revealed that this kinase appears to have a distinct and punctate distribution within the plant nucleus and nucleolus. Dual probing of root sections with JIM135 and anti–bromo-UTP antibodies, after in vitro transcription had been allowed to proceed in the presence of bromo-UTP, showed that SPI3K-5p associates with active nuclear and nucleolar transcription sites. These findings suggest a possible link between PtdIns 3-kinase activity and nuclear transcription in plants.

Vps Factors Are Required for Efficient Transcription Elongation in Budding Yeast.
N. A. Gaur, J. Hasek, D. G. Brickner, H. Qiu, F. Zhang, C.-M. Wong, I. Malcova, P. Vasicova, J. H. Brickner, and A. G. Hinnebusch (2013)
Genetics 193, 829-851
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Inositol Polyphosphate 5-Phosphatase7 Regulates the Production of Reactive Oxygen Species and Salt Tolerance in Arabidopsis.
Y. Kaye, Y. Golani, Y. Singer, Y. Leshem, G. Cohen, M. Ercetin, G. Gillaspy, and A. Levine (2011)
Plant Physiology 157, 229-241
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Energy Signaling in the Regulation of Gene Expression during Stress.
E. Baena-Gonzalez (2010)
Mol Plant 3, 300-313
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The Arabidopsis Phosphatidylinositol 3-Kinase Is Important for Pollen Development.
Y. Lee, E.-S. Kim, Y. Choi, I. Hwang, C. J. Staiger, Y.-Y. Chung, and Y. Lee (2008)
Plant Physiology 147, 1886-1897
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Stress-inducible and constitutive phosphoinositide pools have distinctive fatty acid patterns in Arabidopsis thaliana.
S. Konig, A. Mosblech, and I. Heilmann (2007)
FASEB J 21, 1958-1967
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TbVps34, the Trypanosome Orthologue of Vps34, Is Required for Golgi Complex Segregation.
B. S. Hall, C. Gabernet-Castello, A. Voak, D. Goulding, S. K. Natesan, and M. C. Field (2006)
J. Biol. Chem. 281, 27600-27612
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Gene silencing reveals a specific function of hVps34 phosphatidylinositol 3-kinase in late versus early endosomes.
E. E. Johnson, J. H. Overmeyer, W. T. Gunning, and W. A. Maltese (2006)
J. Cell Sci. 119, 1219-1232
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PIKE/nuclear PI 3-kinase signaling mediates the antiapoptotic actions of NGF in the nucleus.
J.-Y. Ahn, R. Rong, X. Liu, and K. Ye (2004)
EMBO J. 23, 3995-4006
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Novel expression patterns of phosphatidylinositol 3-hydroxy kinase in nodulated Medicago spp. plants.
L. E. Hernandez, C. Escobar, B. K. Drobak, T. Bisseling, and N. J. Brewin (2004)
J. Exp. Bot. 55, 957-959
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Differential Regulation of Two Arabidopsis Type III Phosphatidylinositol 4-Kinase Isoforms. A Regulatory Role for the Pleckstrin Homology Domain.
J. Stevenson-Paulik, J. Love, and W. F. Boss (2003)
Plant Physiology 132, 1053-1064
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A Role for Phosphatidylinositol 3-Phosphate in Abscisic Acid-Induced Reactive Oxygen Species Generation in Guard Cells.
K.-Y. Park, J.-Y. Jung, J. Park, J.-U. Hwang, Y.-W. Kim, I. Hwang, and Y. Lee (2003)
Plant Physiology 132, 92-98
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Arabidopsis Inositol Polyphosphate 6-/3-Kinase Is a Nuclear Protein That Complements a Yeast Mutant Lacking a Functional ArgR-Mcm1 Transcription Complex.
H.-J. Xia, C. Brearley, S. Elge, B. Kaplan, H. Fromm, and B. Mueller-Roeber (2003)
PLANT CELL 15, 449-463
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Phosphatidylinositol 3- and 4-Phosphate Are Required for Normal Stomatal Movements.
J.-Y. Jung, Y.-W. Kim, J. M. Kwak, J.-U. Hwang, J. Young, J. I. Schroeder, I. Hwang, and Y. Lee (2002)
PLANT CELL 14, 2399-2412
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Nuclear Lipid Signaling.
R. F. Irvine (2002)
Sci. STKE 2002, re13
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Inositol Phospholipid Metabolism in Arabidopsis. Characterized and Putative Isoforms of Inositol Phospholipid Kinase and Phosphoinositide-Specific Phospholipase C.
B. Mueller-Roeber and C. Pical (2002)
Plant Physiology 130, 22-46
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Membrane transport in Caenorhabditis elegans: an essential role for VPS34 at the nuclear membrane.
L. Roggo, V. Bernard, A. L. Kovacs, A. M. Rose, F. Savoy, M. Zetka, M. P. Wymann, and F. Muller (2002)
EMBO J. 21, 1673-1683
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PARF-1: an Arabidopsis thaliana FYVE-domain protein displaying a novel eukaryotic domain structure and phosphoinositide affinity.
B. Heras and B. K. Drobak (2002)
J. Exp. Bot. 53, 565-567
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Plasma Membrane Phosphatidylinositol 4,5-Bisphosphate Levels Decrease with Time in Culture.
I. Heilmann, I. Y. Perera, W. Gross, and W. F. Boss (2001)
Plant Physiology 126, 1507-1518
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Rapid Accumulation of Phosphatidylinositol 4,5-Bisphosphate and Inositol 1,4,5-Trisphosphate Correlates with Calcium Mobilization in Salt-Stressed Arabidopsis.
D. B. DeWald, J. Torabinejad, C. A. Jones, J. C. Shope, A. R. Cangelosi, J. E. Thompson, G. D. Prestwich, and H. Hama (2001)
Plant Physiology 126, 759-769
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