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PLANT CELL 13 (5): 1155-1163

Copyright © 2001 by the American Society of Plant Physiologists.

Plant Cell, Vol. 13, 1155-1163, May 2001, Copyright © 2001, American Society of Plant Physiologists Both the Extracellular Leucine-Rich Repeat Domain and the Kinase Activity of FLS2 Are Required for Flagellin Binding and Signaling in Arabidopsis Lourdes Gómez-Gómeza, Zsuzsa Bauera, and Thomas Bollera,b a Friedrich Miescher-Institut, P.O. Box 2543, CH-4002 Basel, Switzerland b Botanisches Institut, Universität Basel, Helbelstrasse 1, CH-4056 Basel, Switzerland Lourdes Gómez-Gómez, lourdes.gomez{at}fmi.ch (E-mail), 41-61-697-45-27 (fax)

In Arabidopsis, activation of defense responses by flagellin is triggered by the specific recognition of the most conserved domain of flagellin, represented by the peptide flg22, in a process involving the FLS2 gene, which encodes a leucine-rich repeat serine/threonine protein kinase. We show here that the two fls2 mutant alleles, fls2-24 and fls2-17, which were shown previously to confer insensitivity to flg22, also cause impaired flagellin binding. These features are rescued when a functional FLS2 gene is expressed as a transgene in each of the fls2 mutant plants, indicating that FLS2 is necessary for flagellin binding. The point mutation of the fls2-17 allele lies in the kinase domain. A kinase carrying this missense mutation lacked autophosphorylation activity when expressed in Escherichia coli. This indicates that kinase activity is required for binding and probably affects the stability of the flagellin receptor complex. We further show that overexpression of the kinase-associated protein phosphatase (KAPP) in Arabidopsis results in plants that are insensitive to flagellin treatment, and we show reduced flg22 binding in these plants. Furthermore, using the yeast two-hybrid system, we show physical interaction of KAPP with the kinase domain of FLS2. These results suggest that KAPP functions as a negative regulator of the FLS2 signal transduction pathway and that the phosphorylation of FLS2 is necessary for proper binding and signaling of the flagellin receptor complex.

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