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Plant Physiology 126 (3): 1055-1060

Copyright © 2001 by the American Society of Plant Physiologists.

Plant Physiol, July 2001, Vol. 126, pp. 1055-1060

Role of Auxin-Induced Reactive Oxygen Species in Root Gravitropism1

Jung Hee Joo, Yun Soo Bae, and June Seung Lee*

Center for Cell Signaling Research, Department of Biological Sciences, Ewha Womans University, Seoul 120-750, South Korea

We report our studies on root gravitropism indicating that reactive oxygen species (ROS) may function as a downstream component in auxin-mediated signal transduction. A transient increase in the intracellular concentration of ROS in the convex endodermis resulted from either gravistimulation or unilateral application of auxin to vertical roots. Root bending was also brought about by unilateral application of ROS to vertical roots pretreated with the auxin transport inhibitor N-1-naphthylphthalamic acid. Furthermore, the scavenging of ROS by antioxidants (N-acetylcysteine, ascorbic acid, and Trolox) inhibited root gravitropism. These results indicate that the generation of ROS plays a role in root gravitropism.

1 This work was supported by the Center of Excellence grant (to J.S.L. and Y.S.B.), by the BK21 program (to Y.S.B.) from Ministry of Education, and by the Korea Research Foundation (grant to J.S.L.) made in the program year of 1998.

* Corresponding author; e-mail jslee{at}; fax 82-2-3277-3760.

© 2001 American Society of Plant Physiologists

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