Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Subscribe

Logo for

Plant Physiology 126 (3): 1055-1060

Copyright © 2001 by the American Society of Plant Physiologists.

Plant Physiol, July 2001, Vol. 126, pp. 1055-1060

Role of Auxin-Induced Reactive Oxygen Species in Root Gravitropism1

Jung Hee Joo, Yun Soo Bae, and June Seung Lee*

Center for Cell Signaling Research, Department of Biological Sciences, Ewha Womans University, Seoul 120-750, South Korea

We report our studies on root gravitropism indicating that reactive oxygen species (ROS) may function as a downstream component in auxin-mediated signal transduction. A transient increase in the intracellular concentration of ROS in the convex endodermis resulted from either gravistimulation or unilateral application of auxin to vertical roots. Root bending was also brought about by unilateral application of ROS to vertical roots pretreated with the auxin transport inhibitor N-1-naphthylphthalamic acid. Furthermore, the scavenging of ROS by antioxidants (N-acetylcysteine, ascorbic acid, and Trolox) inhibited root gravitropism. These results indicate that the generation of ROS plays a role in root gravitropism.

1 This work was supported by the Center of Excellence grant (to J.S.L. and Y.S.B.), by the BK21 program (to Y.S.B.) from Ministry of Education, and by the Korea Research Foundation (grant to J.S.L.) made in the program year of 1998.

* Corresponding author; e-mail jslee{at}mm.ewha.ac.kr; fax 82-2-3277-3760.

© 2001 American Society of Plant Physiologists

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Heterotrimeric G protein signalling in the plant kingdom.
D. Urano, J.-G. Chen, J. R. Botella, and A. M. Jones (2013)
Open Bio 3, 120186
   Abstract »    Full Text »    PDF »
Transient Influx of Nickel in Root Mitochondria Modulates Organic Acid and Reactive Oxygen Species Production in Nickel Hyperaccumulator Alyssum murale.
B. Agrawal, K. J. Czymmek, D. L. Sparks, and H. P. Bais (2013)
J. Biol. Chem. 288, 7351-7362
   Abstract »    Full Text »    PDF »
Enhanced Reactive Oxygen Species Scavenging by Overproduction of Superoxide Dismutase and Catalase Delays Postharvest Physiological Deterioration of Cassava Storage Roots.
J. Xu, X. Duan, J. Yang, J. R. Beeching, and P. Zhang (2013)
Plant Physiology 161, 1517-1528
   Abstract »    Full Text »    PDF »
Nitrated Cyclic GMP Modulates Guard Cell Signaling in Arabidopsis.
T. Joudoi, Y. Shichiri, N. Kamizono, T. Akaike, T. Sawa, J. Yoshitake, N. Yamada, and S. Iwai (2013)
PLANT CELL 25, 558-571
   Abstract »    Full Text »    PDF »
A role for lipid-mediated signaling in plant gravitropism.
C. M. Smith, M. Desai, E. S. Land, and I. Y. Perera (2013)
Am. J. Botany 100, 153-160
   Abstract »    Full Text »    PDF »
A proteomics approach identifies novel proteins involved in gravitropic signal transduction.
C. A. Schenck, V. Nadella, S. L. Clay, J. Lindner, Z. Abrams, and S. E. Wyatt (2013)
Am. J. Botany 100, 194-202
   Abstract »    Full Text »    PDF »
Gravitropism and mechanical signaling in plants.
M. Toyota and S. Gilroy (2013)
Am. J. Botany 100, 111-125
   Abstract »    Full Text »    PDF »
Gravity Sensing and Signal Transduction in Vascular Plant Primary Roots.
K. L. Baldwin, A. K. Strohm, and P. H. Masson (2013)
Am. J. Botany 100, 126-142
   Abstract »    Full Text »    PDF »
Cereal Grass Pulvini: Agronomically Significant Models for Studying Gravitropism Signaling and Tissue Polarity.
A. M. Clore (2013)
Am. J. Botany 100, 101-110
   Abstract »    Full Text »    PDF »
The proteome of Populus nigra woody root: response to bending.
D. Trupiano, M. Rocco, G. Renzone, A. Scaloni, V. Viscosi, D. Chiatante, and G. S. Scippa (2012)
Ann. Bot. 110, 415-432
   Abstract »    Full Text »    PDF »
N-3-Oxo-Decanoyl-L-Homoserine-Lactone Activates Auxin-Induced Adventitious Root Formation via Hydrogen Peroxide- and Nitric Oxide-Dependent Cyclic GMP Signaling in Mung Bean.
X. Bai, C. D. Todd, R. Desikan, Y. Yang, and X. Hu (2012)
Plant Physiology 158, 725-736
   Abstract »    Full Text »    PDF »
Extracellular Nucleotides and Apyrases Regulate Stomatal Aperture in Arabidopsis.
G. Clark, D. Fraley, I. Steinebrunner, A. Cervantes, J. Onyirimba, A. Liu, J. Torres, W. Tang, J. Kim, and S. J. Roux (2011)
Plant Physiology 156, 1740-1753
   Abstract »    Full Text »    PDF »
Arabidopsis Monothiol Glutaredoxin, AtGRXS17, Is Critical for Temperature-dependent Postembryonic Growth and Development via Modulating Auxin Response.
N.-H. Cheng, J.-Z. Liu, X. Liu, Q. Wu, S. M. Thompson, J. Lin, J. Chang, S. A. Whitham, S. Park, J. D. Cohen, et al. (2011)
J. Biol. Chem. 286, 20398-20406
   Abstract »    Full Text »    PDF »
Cell Wall Integrity Controls Root Elongation via a General 1-Aminocyclopropane-1-Carboxylic Acid-Dependent, Ethylene-Independent Pathway.
D. L. Tsang, C. Edmond, J. L. Harrington, and T. S. Nuhse (2011)
Plant Physiology 156, 596-604
   Abstract »    Full Text »    PDF »
Intercellular Communication during Plant Development.
J. M. Van Norman, N. W. Breakfield, and P. N. Benfey (2011)
PLANT CELL 23, 855-864
   Abstract »    Full Text »    PDF »
Ralstonia solanacearum Dps Contributes to Oxidative Stress Tolerance and to Colonization of and Virulence on Tomato Plants.
J. M. Colburn-Clifford, J. M. Scherf, and C. Allen (2010)
Appl. Envir. Microbiol. 76, 7392-7399
   Abstract »    Full Text »    PDF »
FERONIA receptor-like kinase regulates RHO GTPase signaling of root hair development.
Q. Duan, D. Kita, C. Li, A. Y. Cheung, and H.-M. Wu (2010)
PNAS 107, 17821-17826
   Abstract »    Full Text »    PDF »
Effect of ectomycorrhizal colonization and drought on reactive oxygen species metabolism of Nothofagus dombeyi roots.
M. Alvarez, D. Huygens, C. Fernandez, Y. Gacitua, E. Olivares, I. Saavedra, M. Alberdi, and E. Valenzuela (2009)
Tree Physiol 29, 1047-1057
   Abstract »    Full Text »    PDF »
Reductions in Maize Root-tip Elongation by Salt and Osmotic Stress do not Correlate with Apoplastic O2*- Levels.
D. Bustos, R. Lascano, A. L. Villasuso, E. Machado, M. E. Senn, A. Cordoba, and E. Taleisnik (2008)
Ann. Bot. 102, 551-559
   Abstract »    Full Text »    PDF »
Galactinol and Raffinose Constitute a Novel Function to Protect Plants from Oxidative Damage.
A. Nishizawa, Y. Yabuta, and S. Shigeoka (2008)
Plant Physiology 147, 1251-1263
   Abstract »    Full Text »    PDF »
Involvement of Polyamine Oxidase in Wound Healing.
R. Angelini, A. Tisi, G. Rea, M. M. Chen, M. Botta, R. Federico, and A. Cona (2008)
Plant Physiology 146, 162-177
   Abstract »    Full Text »    PDF »
Time-course of changes in amounts of specific proteins upon exposure to hyper-g, 2-D clinorotation, and 3-D random positioning of Arabidopsis cell cultures.
Z. Barjaktarovic, A. Nordheim, T. Lamkemeyer, C. Fladerer, J. Madlung, and R. Hampp (2007)
J. Exp. Bot. 58, 4357-4363
   Abstract »    Full Text »    PDF »
Proteomics Identification of Differentially Expressed Proteins Associated with Pollen Germination and Tube Growth Reveals Characteristics of Germinated Oryza sativa Pollen.
S. Dai, T. Chen, K. Chong, Y. Xue, S. Liu, and T. Wang (2007)
Mol. Cell. Proteomics 6, 207-230
   Abstract »    Full Text »    PDF »
Factors involved in root formation in Medicago truncatula.
N. Imin, M. Nizamidin, T. Wu, and B. G. Rolfe (2007)
J. Exp. Bot. 58, 439-451
   Abstract »    Full Text »    PDF »
Redox Regulatory Mechanisms in Cellular Stress Responses.
N. FEDOROFF (2006)
Ann. Bot. 98, 289-300
   Abstract »    Full Text »    PDF »
Flavin-containing polyamine oxidase is a hydrogen peroxide source in the oxidative response to the protein phosphatase inhibitor cantharidin in Zea mays L..
A. Cona, G. Rea, M. Botta, F. Corelli, R. Federico, and R. Angelini (2006)
J. Exp. Bot. 57, 2277-2289
   Abstract »    Full Text »    PDF »
The Role of Reactive Oxygen Species in Hormonal Responses.
J. M. Kwak, V. Nguyen, and J. I. Schroeder (2006)
Plant Physiology 141, 323-329
   Full Text »    PDF »
Expression and Characterization of a Redox-Sensing Green Fluorescent Protein (Reduction-Oxidation-Sensitive Green Fluorescent Protein) in Arabidopsis.
K. Jiang, C. Schwarzer, E. Lally, S. Zhang, S. Ruzin, T. Machen, S. J. Remington, and L. Feldman (2006)
Plant Physiology 141, 397-403
   Abstract »    Full Text »    PDF »
Ascorbate Oxidase-Dependent Changes in the Redox State of the Apoplast Modulate Gene Transcript Accumulation Leading to Modified Hormone Signaling and Orchestration of Defense Processes in Tobacco.
C. Pignocchi, G. Kiddle, I. Hernandez, S. J. Foster, A. Asensi, T. Taybi, J. Barnes, and C. H. Foyer (2006)
Plant Physiology 141, 423-435
   Abstract »    Full Text »    PDF »
Modulation of plant morphology, root architecture, and cell structure by low vitamin C in Arabidopsis thaliana.
E Olmos, G Kiddle, T. Pellny, S Kumar, and C. Foyer (2006)
J. Exp. Bot. 57, 1645-1655
   Abstract »    Full Text »    PDF »
Ascorbic Acid Deficiency Activates Cell Death and Disease Resistance Responses in Arabidopsis.
V. Pavet, E. Olmos, G. Kiddle, S. Mowla, S. Kumar, J. Antoniw, M. E. Alvarez, and C. H. Foyer (2005)
Plant Physiology 139, 1291-1303
   Abstract »    Full Text »    PDF »
Complementary interactions between oxidative stress and auxins control plant growth responses at plant, organ, and cellular level.
T. Pasternak, G. Potters, R. Caubergs, and M. A. K. Jansen (2005)
J. Exp. Bot. 56, 1991-2001
   Abstract »    Full Text »    PDF »
Acclimation to Diverse Environmental Stresses Caused by a Suppression of Cytosolic Ascorbate Peroxidase in Tobacco BY-2 cells.
T. Ishikawa, Y. Morimoto, R. Madhusudhan, Y. Sawa, H. Shibata, Y. Yabuta, A. Nishizawa, and S. Shigeoka (2005)
Plant Cell Physiol. 46, 1264-1271
   Abstract »    Full Text »    PDF »
Different Signaling and Cell Death Roles of Heterotrimeric G Protein {alpha} and {beta} Subunits in the Arabidopsis Oxidative Stress Response to Ozone.
J. H. Joo, S. Wang, J.G. Chen, A.M. Jones, and N. V. Fedoroff (2005)
PLANT CELL 17, 957-970
   Abstract »    Full Text »    PDF »
Nitric Oxide Mediates Gravitropic Bending in Soybean Roots.
X. Hu, S. J. Neill, Z. Tang, and W. Cai (2005)
Plant Physiology 137, 663-670
   Abstract »    Full Text »    PDF »
The Fast and Transient Transcriptional Network of Gravity and Mechanical Stimulation in the Arabidopsis Root Apex.
J. M. Kimbrough, R. Salinas-Mondragon, W. F. Boss, C. S. Brown, and H. W. Sederoff (2004)
Plant Physiology 136, 2790-2805
   Abstract »    Full Text »    PDF »
Arabidopsis RADICAL-INDUCED CELL DEATH1 Belongs to the WWE Protein-Protein Interaction Domain Protein Family and Modulates Abscisic Acid, Ethylene, and Methyl Jasmonate Responses.
R. Ahlfors, S. Lang, K. Overmyer, P. Jaspers, M. Brosche, A. Tauriainen, H. Kollist, H. Tuominen, E. Belles-Boix, M. Piippo, et al. (2004)
PLANT CELL 16, 1925-1937
   Abstract »    Full Text »    PDF »
Hydrogen peroxide mediates plant root cell response to nutrient deprivation.
R. Shin and D. P. Schachtman (2004)
PNAS 101, 8827-8832
   Abstract »    Full Text »    PDF »
Reactive Oxygen Species Activation of Plant Ca2+ Channels. A Signaling Mechanism in Polar Growth, Hormone Transduction, Stress Signaling, and Hypothetically Mechanotransduction.
I. C. Mori and J. I. Schroeder (2004)
Plant Physiology 135, 702-708
   Full Text »    PDF »
Plant Respiratory Burst Oxidase Homologs Impinge on Wound Responsiveness and Development in Lycopersicon esculentum.
M. Sagi, O. Davydov, S. Orazova, Z. Yesbergenova, R. Ophir, J. W. Stratmann, and R. Fluhr (2004)
PLANT CELL 16, 616-628
   Abstract »    Full Text »    PDF »
Plant Gravitropism. Unraveling the Ups and Downs of a Complex Process.
E. B. Blancaflor and P. H. Masson (2003)
Plant Physiology 133, 1677-1690
   Full Text »    PDF »
The Function of Ascorbate Oxidase in Tobacco.
C. Pignocchi, J. M. Fletcher, J. E. Wilkinson, J. D. Barnes, and C. H. Foyer (2003)
Plant Physiology 132, 1631-1641
   Abstract »    Full Text »    PDF »
A Role for Phosphatidylinositol 3-Phosphate in Abscisic Acid-Induced Reactive Oxygen Species Generation in Guard Cells.
K.-Y. Park, J.-Y. Jung, J. Park, J.-U. Hwang, Y.-W. Kim, I. Hwang, and Y. Lee (2003)
Plant Physiology 132, 92-98
   Abstract »    Full Text »    PDF »
Enhanced Gravitropism of Roots with a Disrupted Cap Actin Cytoskeleton.
G. Hou, D. R. Mohamalawari, and E. B. Blancaflor (2003)
Plant Physiology 131, 1360-1373
   Abstract »    Full Text »    PDF »
Free oxygen radicals regulate plasma membrane Ca2+- and K+-permeable channels in plant root cells.
V. Demidchik, S. N. Shabala, K. B. Coutts, M. A. Tester, and J. M. Davies (2003)
J. Cell Sci. 116, 81-88
   Abstract »    Full Text »    PDF »
The as-1 Promoter Element Is an Oxidative Stress-Responsive Element and Salicylic Acid Activates It via Oxidative Species.
V. Garreton, J. Carpinelli, X. Jordana, and L. Holuigue (2002)
Plant Physiology 130, 1516-1526
   Abstract »    Full Text »    PDF »
Transcription Profiling of the Early Gravitropic Response in Arabidopsis Using High-Density Oligonucleotide Probe Microarrays.
N. Moseyko, T. Zhu, H.-S. Chang, X. Wang, and L. J. Feldman (2002)
Plant Physiology 130, 720-728
   Abstract »    Full Text »    PDF »
Reactive Oxygen Species in the Elongation Zone of Maize Leaves Are Necessary for Leaf Extension.
A. A. Rodriguez, K. A. Grunberg, and E. L. Taleisnik (2002)
Plant Physiology 129, 1627-1632
   Abstract »    Full Text »    PDF »
Common Components, Networks, and Pathways of Cross-Tolerance to Stress. The Central Role of "Redox" and Abscisic Acid-Mediated Controls.
G. M. Pastori and C. H. Foyer (2002)
Plant Physiology 129, 460-468
   Full Text »    PDF »
Signal transduction during oxidative stress.
E. Vranova, D. Inze, and F. Van Breusegem (2002)
J. Exp. Bot. 53, 1227-1236
   Abstract »    Full Text »    PDF »
Small GTPases: Versatile Signaling Switches in Plants.
Z. Yang (2002)
PLANT CELL 14, S375-S388
   Full Text »    PDF »
Dancing Together. Social Controls in Parasitic Plant Development.
W. J. Keyes, J. V. Taylor, R. P. Apkarian, and D. G. Lynn (2001)
Plant Physiology 127, 1508-1512
   Full Text »    PDF »

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882