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Plant Physiology 126 (4): 1507-1518

Copyright © 2001 by the American Society of Plant Physiologists.

Plant Physiol, August 2001, Vol. 126, pp. 1507-1518

Plasma Membrane Phosphatidylinositol 4,5-Bisphosphate Levels Decrease with Time in Culture1

Ingo Heilmann, Imara Y. Perera, Wolfgang Gross, and Wendy F. Boss*

Department of Botany, North Carolina State University, Raleigh, NC 27695-7612 (I.H., I.Y.P., W.F.B.); and Institut für Biologie (Pflanzenphysiologie), Freie Universität, Berlin, Germany (W.G.)

During the stationary phase of growth, after 7 to 12 d in culture, the levels of phosphatidylinositol 4,5-bisphosphate (PtdInsP2) decreased by 75% in plasma membranes of the red alga Galdieria sulphuraria. Concomitant with the decrease in PtdInsP2 levels in plasma membranes, there was an increase in PtdInsP2 in microsomes, suggesting that the levels of plasma membrane PtdInsP2 are regulated differentially. The decline of PtdInsP2 in plasma membranes was accompanied by a 70% decrease in the specific activity of PtdInsP kinase and by reduced levels of protein cross-reacting with antisera against a conserved PtdInsP kinase domain. Upon osmotic stimulation, the loss of PtdInsP2from the plasma membrane increased from 10% in 7-d-old cells to 60% in 12-d-old cells, although the levels of inositol 1,4,5-trisphosphate (InsP3) produced in whole cells were roughly equal at both times. When cells with low plasma membrane PtdInsP2 levels were osmotically stimulated, a mild osmotic stress (12.5 mM KCl) activated PtdInsP kinase prior to InsP3 production, whereas in cells with high plasma membrane PtdInsP2, more severe stress (250 mM KCl) was required to induce an increase in PtdInsP kinase activity. The differential regulation of a plasma membrane signaling pool of PtdInsP2 is discussed with regard to the implications for understanding the responsive state of cells.

1 This work was supported by the National Science Foundation (grant no. MCB-9604285 to W.F.B.) and by a Deutscher Akademischer Austauschdienst fellowship (HSPIII to I.H.) financed by the German Federal Ministry of Education, Science, Research, and Technology.

* Corresponding author; email wendy_boss{at}ncsu.edu; fax 919-515-3436.

© 2001 American Society of Plant Physiologists

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