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PNAS 96 (19): 10626-10631

Copyright © 1999 by the National Academy of Sciences.

BIOLOGICAL SCIENCES / BIOCHEMISTRY

Crosstalk between Gαi- and Gαq-coupled receptors is mediated by Gβ{gamma} exchange

Ursula Quitterer Martin J. Lohse*

Institut für Pharmakologie und Toxikologie der Universität Würzburg, Versbacher Strasse 9, 97078 Würzburg, Germany

Accepted for publication July 13, 1999.

Received for publication October 20, 1998.

Abstract: Activation of Gαi-coupled receptors often causes enhancement of the inositol phosphate signal triggered by Gαq-coupled receptors. To investigate the mechanism of this synergistic receptor crosstalk, we studied the Gαi-coupled adenosine A1 and α2C adrenergic receptors and the Gαq-coupled bradykinin B2 and a UTP-preferring P2Y receptor. Stimulation of either Gαi-coupled receptor expressed in COS cells increased the potency and the efficacy of inositol phosphate production by bradykinin or UTP. Likewise, overexpression of Gβ1{gamma}2 resulted in a similar increase in potency and efficacy of bradykinin or UTP. In contrast, these stimuli did not affect the potency of direct activators of Gαq; a truncated Gβ3 mutant had no effect on the receptor-generated signals whereas signals generated at the G-protein level were still enhanced. This suggests that the Gβ{gamma}-mediated signal enhancement occurs at the receptor level. Almost all possible combinations of Gβ1–3 with G{gamma}2–7 were equally effective in enhancing the signals of the B2 and a UTP-preferring P2Y receptor, indicating a very broad specificity of this synergism. The enhancement of the bradykinin signal by (i) Gαi-activating receptor ligands or (ii) cotransfection of Gβ{gamma} was suppressed when the B2 receptor was replaced by a B2Gβ2 fusion protein. Gβ{gamma} enhanced the B2 receptor-stimulated activation of G-proteins as determined by GTP{gamma}S-induced decrease in high affinity agonist binding and by B2 receptor-enhanced [35S]GTP{gamma}S binding. These findings support the concept that Gβ{gamma} exchange between Gαi- and Gαq-coupled receptors mediates this type of receptor crosstalk.

* To whom reprint requests should be addressed. E-mail: lohse{at}toxi.uni-wuerzburg.de.

Communicated by Rolf Huisgen, University of Munich, Muenchen, Germany

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