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PNAS 96 (22): 12667-12672

Copyright © 1999 by the National Academy of Sciences.


LFG: An anti-apoptotic gene that provides protection from Fas-mediated cell death

Nikunj V. Somia, Mark J. Schmitt, Douglas E. Vetter, Daniel Van Antwerp, Stephen F. Heinemann, and Inder M. Verma*

The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037

Contributed by Inder M. Verma

Accepted for publication September 3, 1999.

Abstract: Programmed cell death regulates a number of biological phenomena, and the apoptotic signal must itself be tightly controlled to avoid inappropriate cell death. We established a genetic screen to search for molecules that inhibit the apoptotic signal from the Fas receptor. Here we report the isolation of a gene, LFG, that protects cells uniquely from Fas but not from the mechanistically related tumor necrosis factor α death signal. LFG is widely distributed, but remarkably is highly expressed in the hippocampus. LFG can bind to the Fas receptor, but does not regulate Fas expression or interfere with binding of an agonist antibody. Furthermore LFG does not inhibit binding of FADD to Fas.

* To whom reprint requests should be addressed at: Laboratory of Genetics, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037. E-mail: verma{at}

Data deposition: The sequence reported in this paper has been deposited in the GenBank database (accession no. AF190461).

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