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Science 286 (5443): 1362-1365

Copyright © 1999 by the American Association for the Advancement of Science

The Protein Kinase p90 Rsk as an Essential Mediator of Cytostatic Factor Activity

Ramesh R. Bhatt, James E. Ferrell Jr. *

Persistent activation of p42 mitogen-activated protein kinase (p42 MAPK) during mitosis induces a "cytostatic factor" arrest, the arrest responsible for preventing the parthenogenetic activation of unfertilized eggs. The protein kinase p90 Rsk is a substrate of p42 MAPK; thus, the role of p90 Rsk in p42 MAPK-induced mitotic arrest was examined. Xenopus laevis egg extracts immunodepleted of Rsk lost their capacity to undergo mitotic arrest in response to activation of the Mos-MEK-1-p42 MAPK cascade of protein kinases. Replenishing Rsk-depleted extracts with catalytically competent Rsk protein restored the ability of the extracts to undergo mitotic arrest. Rsk appears to be essential for cytostatic factor arrest.

Department of Molecular Pharmacology, Stanford University School of Medicine, Stanford, CA 94305-5332, USA.
*   To whom correspondence should be addressed. E-mail: ferrell{at}cmgm.stanford.edu


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