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Science 288 (5470): 1439-1441

Copyright © 2000 by the American Association for the Advancement of Science

Regulation of B Lymphocyte and Macrophage Development by Graded Expression of PU.1

Rodney P. DeKoter, Harinder Singh *

The ets family transcription factor PU.1 is required for the development of multiple lineages of the immune system. Using retroviral transduction of PU.1 complementary DNA into mutant hematopoietic progenitors, we demonstrate that differing concentrations of the protein regulate the development of B lymphocytes as compared with macrophages. A low concentration of PU.1 protein induces the B cell fate, whereas a high concentration promotes macrophage differentiation and blocks B cell development. Conversely, a transcriptionally weakened mutant protein preferentially induces B cell generation. Our results suggest that graded expression of a transcription factor can be used to specify distinct cell fates in the hematopoietic system.

Howard Hughes Medical Institute and Department of Molecular Genetics and Cell Biology, The University of Chicago, 5841 South Maryland Avenue, MC1028, Chicago, IL 60637, USA.
*   To whom correspondence should be addressed. E-mail: hsingh{at}

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E. Bailey, L. Li, A. S. Duffield, H. S. Ma, D. L. Huso, and D. Small (2013)
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   Abstract »    Full Text »    PDF »
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J. Ahsberg, J. Ungerback, T. Strid, E. Welinder, J. Stjernberg, M. Larsson, H. Qian, and M. Sigvardsson (2013)
J. Biol. Chem. 288, 33449-33461
   Abstract »    Full Text »    PDF »
Transcriptional Regulation of IL-15 Expression during Hematopoiesis.
S. L. Colpitts, S. W. Stonier, T. A. Stoklasek, S. H. Root, H. L. Aguila, K. S. Schluns, and L. Lefrancois (2013)
J. Immunol. 191, 3017-3024
   Abstract »    Full Text »    PDF »
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J. R. DiSpirito, B. Fang, F. Wang, and M. A. Lazar (2013)
Mol. Cell. Biol. 33, 3354-3364
   Abstract »    Full Text »    PDF »
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H. Y. Kueh, A. Champhekar, S. L. Nutt, M. B. Elowitz, and E. V. Rothenberg (2013)
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   Abstract »    Full Text »    PDF »
Hacking cell differentiation: transcriptional rerouting in reprogramming, lineage infidelity and metaplasia.
G. Regalo and A. Leutz (2013)
EMBO Mol Med. 5, 1154-1164
   Abstract »    Full Text »    PDF »
Mechanisms of in vivo binding site selection of the hematopoietic master transcription factor PU.1.
T.-H. Pham, J. Minderjahn, C. Schmidl, H. Hoffmeister, S. Schmidhofer, W. Chen, G. Langst, C. Benner, and M. Rehli (2013)
Nucleic Acids Res. 41, 6391-6402
   Abstract »    Full Text »    PDF »
Transcription factor dose links development to disease.
M. Sigvardsson (2012)
Blood 120, 3630-3631
   Full Text »    PDF »
Biphenotypic B-lymphoid/myeloid cells expressing low levels of Pax5: potential targets of BAL development.
S. Simmons, M. Knoll, C. Drewell, I. Wolf, H.-J. Mollenkopf, C. Bouquet, and F. Melchers (2012)
Blood 120, 3688-3698
   Abstract »    Full Text »    PDF »
Transcription Factor-mediated Epigenetic Reprogramming.
J. Biol. Chem. 287, 30922-30931
ChIP-seq guidelines and practices of the ENCODE and modENCODE consortia.
S. G. Landt, G. K. Marinov, A. Kundaje, P. Kheradpour, F. Pauli, S. Batzoglou, B. E. Bernstein, P. Bickel, J. B. Brown, P. Cayting, et al. (2012)
Genome Res. 22, 1813-1831
   Abstract »    Full Text »    PDF »
Plenary Perspective: The complexity of constitutive and inducible gene expression in mononuclear phagocytes.
D. A. Hume (2012)
J. Leukoc. Biol. 92, 433-444
   Abstract »    Full Text »    PDF »
Functional PU.1 in macrophages has a pivotal role in NF-{kappa}B activation and neutrophilic lung inflammation during endotoxemia.
M. Karpurapu, X. Wang, J. Deng, H. Park, L. Xiao, R. T. Sadikot, R. S. Frey, U. A. Maus, G. Y. Park, E. W. Scott, et al. (2011)
Blood 118, 5255-5266
   Abstract »    Full Text »    PDF »
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K. Arita, S. Endo, T. Kaifu, K. Kitaguchi, A. Nakamura, H. Ohmori, K. Kohu, M. Satake, and T. Takai (2011)
J. Immunol. 186, 7050-7059
   Abstract »    Full Text »    PDF »
A microRNA expression signature of osteoclastogenesis.
T. Sugatani, J. Vacher, and K. A. Hruska (2011)
Blood 117, 3648-3657
   Abstract »    Full Text »    PDF »
The ARID Family Transcription Factor Bright Is Required for both Hematopoietic Stem Cell and B Lineage Development.
C. F. Webb, J. Bryant, M. Popowski, L. Allred, D. Kim, J. Harriss, C. Schmidt, C. A. Miner, K. Rose, H.-L. Cheng, et al. (2011)
Mol. Cell. Biol. 31, 1041-1053
   Abstract »    Full Text »    PDF »
Accurate inference of transcription factor binding from DNA sequence and chromatin accessibility data.
R. Pique-Regi, J. F. Degner, A. A. Pai, D. J. Gaffney, Y. Gilad, and J. K. Pritchard (2011)
Genome Res. 21, 447-455
   Abstract »    Full Text »    PDF »
The genomic landscapes of inflammation.
G. Natoli, S. Ghisletti, and I. Barozzi (2011)
Genes & Dev. 25, 101-106
   Abstract »    Full Text »    PDF »
Highly conserved transcriptional responses to mechanical ventilation of the lung.
K. S. Kompass, G. Deslee, C. Moore, D. McCurnin, and R. A. Pierce (2010)
Physiol Genomics 42, 384-396
   Abstract »    Full Text »    PDF »
Macrophage-specific gene functions in Spi1-directed innate immunity.
A. Zakrzewska, C. Cui, O. W. Stockhammer, E. L. Benard, H. P. Spaink, and A. H. Meijer (2010)
Blood 116, e1-e11
   Abstract »    Full Text »    PDF »
The Justy mutation identifies Gon4-like as a gene that is essential for B lymphopoiesis.
P. Lu, I. L. Hankel, J. Knisz, A. Marquardt, M.-Y. Chiang, J. Grosse, R. Constien, T. Meyer, A. Schroeder, L. Zeitlmann, et al. (2010)
J. Exp. Med. 207, 1359-1367
   Abstract »    Full Text »    PDF »
PU.1 Binds to a Distal Regulatory Element That Is Necessary for B Cell-Specific Expression of CIITA.
H. Yoon and J. M. Boss (2010)
J. Immunol. 184, 5018-5028
   Abstract »    Full Text »    PDF »
PHOX2A regulation of oculomotor complex nucleogenesis.
K. B. Hasan, S. Agarwala, and C. W. Ragsdale (2010)
Development 137, 1205-1213
   Abstract »    Full Text »    PDF »
Downregulation of PU.1 Leads to Decreased Expression of Dectin-1 in Alveolar Macrophages during Pneumocystis Pneumonia.
C. Zhang, S.-H. Wang, C.-P. Liao, S. Shao, M. E. Lasbury, P. J. Durant, and C.-H. Lee (2010)
Infect. Immun. 78, 1058-1065
   Abstract »    Full Text »    PDF »
Transformation of Chronic Lymphocytic Leukemia/Small Lymphocytic Lymphoma to Interdigitating Dendritic Cell Sarcoma: Evidence for Transdifferentiation of the Lymphoma Clone.
C. R. Fraser, W. Wang, M. Gomez, T. Zhang, S. Mathew, R. R. Furman, D. M. Knowles, A. Orazi, and W. Tam (2009)
Am J Clin Pathol 132, 928-939
   Abstract »    Full Text »    PDF »
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Z. Yang, T. Kondo, C. S. Voorhorst, S. C. Nabinger, L. Ndong, F. Yin, E. M. Chan, M. Yu, O. Wurstlin, C. P. Kratz, et al. (2009)
Mol. Cell. Biol. 29, 4376-4393
   Abstract »    Full Text »    PDF »
Graded repression of PU.1/Sfpi1 gene transcription by GATA factors regulates hematopoietic cell fate.
S. T. Chou, E. Khandros, L. C. Bailey, K. E. Nichols, C. R. Vakoc, Y. Yao, Z. Huang, J. D. Crispino, R. C. Hardison, G. A. Blobel, et al. (2009)
Blood 114, 983-994
   Abstract »    Full Text »    PDF »
Roles of PU.1 in monocyte- and mast cell-specific gene regulation: PU.1 transactivates CIITA pIV in cooperation with IFN-{gamma}.
T. Ito, C. Nishiyama, N. Nakano, M. Nishiyama, Y. Usui, K. Takeda, S. Kanada, K. Fukuyama, H. Akiba, T. Tokura, et al. (2009)
Int. Immunol. 21, 803-816
   Abstract »    Full Text »    PDF »
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K. Maeda, A. Malykhin, B. N. Teague-Weber, X.-H. Sun, A. D. Farris, and K. M. Coggeshall (2009)
Blood 113, 4534-4540
   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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L. Laricchia-Robbio, K. Premanand, C. R. Rinaldi, and G. Nucifora (2009)
Cancer Res. 69, 1633-1642
   Abstract »    Full Text »    PDF »
Balance between Id and E proteins regulates myeloid-versus-lymphoid lineage decisions.
S. W. Cochrane, Y. Zhao, R. S. Welner, and X.-H. Sun (2009)
Blood 113, 1016-1026
   Abstract »    Full Text »    PDF »
IRF8 regulates B-cell lineage specification, commitment, and differentiation.
H. Wang, C. H. Lee, C. Qi, P. Tailor, J. Feng, S. Abbasi, T. Atsumi, and H. C. Morse III (2008)
Blood 112, 4028-4038
   Abstract »    Full Text »    PDF »
B-lineage commitment prior to surface expression of B220 and CD19 on hematopoietic progenitor cells.
R. Mansson, S. Zandi, K. Anderson, I.-L. Martensson, S. E. W. Jacobsen, D. Bryder, and M. Sigvardsson (2008)
Blood 112, 1048-1055
   Abstract »    Full Text »    PDF »
PU.1 expression is modulated by the balance of functional sense and antisense RNAs regulated by a shared cis-regulatory element.
A. K. Ebralidze, F. C. Guibal, U. Steidl, P. Zhang, S. Lee, B. Bartholdy, M. A. Jorda, V. Petkova, F. Rosenbauer, G. Huang, et al. (2008)
Genes & Dev. 22, 2085-2092
   Abstract »    Full Text »    PDF »
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H. Singh (2008)
J. Immunol. 181, 1595-1596
   Full Text »    PDF »
C/EBP{alpha} binds and activates the PU.1 distal enhancer to induce monocyte lineage commitment.
C. Yeamans, D. Wang, I. Paz-Priel, B. E. Torbett, D. G. Tenen, and A. D. Friedman (2007)
Blood 110, 3136-3142
   Abstract »    Full Text »    PDF »
Growth factor independence-1 (Gfi-1) plays a role in mediating specific granule deficiency (SGD) in a patient lacking a gene-inactivating mutation in the C/EBP{epsilon} gene.
A. Khanna-Gupta, H. Sun, T. Zibello, H. M. Lee, R. Dahl, L. A. Boxer, and N. Berliner (2007)
Blood 109, 4181-4190
   Abstract »    Full Text »    PDF »
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A. Lagergren, R. Mansson, J. Zetterblad, E. Smith, B. Basta, D. Bryder, P. Akerblad, and M. Sigvardsson (2007)
J. Biol. Chem. 282, 14454-14462
   Abstract »    Full Text »    PDF »
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R. P. DeKoter, B. L. Schweitzer, M. B. Kamath, D. Jones, H. Tagoh, C. Bonifer, D. A. Hildeman, and K. J. Huang (2007)
J. Biol. Chem. 282, 14194-14204
   Abstract »    Full Text »    PDF »
HOXA10 is a critical regulator for hematopoietic stem cells and erythroid/megakaryocyte development.
M. Magnusson, A. C. M. Brun, N. Miyake, J. Larsson, M. Ehinger, J. M. Bjornsson, A. Wutz, M. Sigvardsson, and S. Karlsson (2007)
Blood 109, 3687-3696
   Abstract »    Full Text »    PDF »
c-Jun Homodimers Can Function as a Context-Specific Coactivator.
B. Grondin, M. Lefrancois, M. Tremblay, M. Saint-Denis, A. Haman, K. Waga, A. Bedard, D. G. Tenen, and T. Hoang (2007)
Mol. Cell. Biol. 27, 2919-2933
   Abstract »    Full Text »    PDF »
Penumbra encodes a novel tetraspanin that is highly expressed in erythroid progenitors and promotes effective erythropoiesis.
M. J. Heikens, T. M. Cao, C. Morita, S. L. DeHart, and S. Tsai (2007)
Blood 109, 3244-3252
   Abstract »    Full Text »    PDF »
Spi-1/PU.1 participates in erythroleukemogenesis by inhibiting apoptosis in cooperation with Epo signaling and by blocking erythroid differentiation.
P. Rimmele, O. Kosmider, P. Mayeux, F. Moreau-Gachelin, and C. Guillouf (2007)
Blood 109, 3007-3014
   Abstract »    Full Text »    PDF »
The Transcriptional Repressor GFI-1 Antagonizes PU.1 Activity through Protein-Protein Interaction.
R. Dahl, S. R. Iyer, K. S. Owens, D. D. Cuylear, and M. C. Simon (2007)
J. Biol. Chem. 282, 6473-6483
   Abstract »    Full Text »    PDF »
PU.1 Activates Transcription of SHP-1 Gene in Hematopoietic Cells.
P. Wlodarski, Q. Zhang, X. Liu, M. Kasprzycka, M. Marzec, and M. A. Wasik (2007)
J. Biol. Chem. 282, 6316-6323
   Abstract »    Full Text »    PDF »
GATA-1-mediated Transcriptional Repression Yields Persistent Transcription Factor IIB-Chromatin Complexes.
M. L. Martowicz, J. A. Grass, and E. H. Bresnick (2006)
J. Biol. Chem. 281, 37345-37352
   Abstract »    Full Text »    PDF »
Stages of Germinal Center Transit Are Defined by B Cell Transcription Factor Coexpression and Relative Abundance.
G. Cattoretti, R. Shaknovich, P. M. Smith, H.-M. Jack, V. V. Murty, and B. Alobeid (2006)
J. Immunol. 177, 6930-6939
   Abstract »    Full Text »    PDF »
The order of expression of transcription factors directs hierarchical specification of hematopoietic lineages..
H. Iwasaki, S.-i. Mizuno, Y. Arinobu, H. Ozawa, Y. Mori, H. Shigematsu, K. Takatsu, D. G. Tenen, and K. Akashi (2006)
Genes & Dev. 20, 3010-3021
   Abstract »    Full Text »    PDF »
Epigenetic Histone Modifications Do Not Control Ig{kappa} Locus Contraction and Intranuclear Localization in Cells with Dual B Cell-Macrophage Potential.
S. Hodawadekar, F. Wei, D. Yu, A. Thomas-Tikhonenko, and M. L. Atchison (2006)
J. Immunol. 177, 6165-6171
   Abstract »    Full Text »    PDF »
Fetal spleen stroma drives macrophage commitment.
J. Y. Bertrand, G. E. Desanti, R. Lo-Man, C. Leclerc, A. Cumano, and R. Golub (2006)
Development 133, 3619-3628
   Abstract »    Full Text »    PDF »
Spi-C Has Opposing Effects to PU.1 on Gene Expression in Progenitor B Cells.
B. L. Schweitzer, K. J. Huang, M. B. Kamath, A. V. Emelyanov, B. K. Birshtein, and R. P. DeKoter (2006)
J. Immunol. 177, 2195-2207
   Abstract »    Full Text »    PDF »
Proper levels of c-Myb are discretely defined at distinct steps of hematopoietic cell development.
H. Sakamoto, G. Dai, K. Tsujino, K. Hashimoto, X. Huang, T. Fujimoto, M. Mucenski, J. Frampton, and M. Ogawa (2006)
Blood 108, 896-903
   Abstract »    Full Text »    PDF »
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D. Wang, C. L. Claus, G. Vaccarelli, M. Braunstein, T. M. Schmitt, J. C. Zuniga-Pflucker, E. V. Rothenberg, and M. K. Anderson (2006)
J. Immunol. 177, 109-119
   Abstract »    Full Text »    PDF »
Differential Binding of Erythroid Krupple-like Factor to Embryonic/Fetal Globin Gene Promoters during Development.
D. Zhou, K. M. Pawlik, J. Ren, C.-W. Sun, and T. M. Townes (2006)
J. Biol. Chem. 281, 16052-16057
   Abstract »    Full Text »    PDF »
C/EBP{alpha} determines hematopoietic cell fate in multipotential progenitor cells by inhibiting erythroid differentiation and inducing myeloid differentiation.
H. C. Suh, J. Gooya, K. Renn, A. D. Friedman, P. F. Johnson, and J. R. Keller (2006)
Blood 107, 4308-4316
   Abstract »    Full Text »    PDF »
SOX2 is a dose-dependent regulator of retinal neural progenitor competence..
O. V. Taranova, S. T. Magness, B. M. Fagan, Y. Wu, N. Surzenko, S. R. Hutton, and L. H. Pevny (2006)
Genes & Dev. 20, 1187-1202
   Abstract »    Full Text »    PDF »
Notch Signaling Requires GATA-2 to Inhibit Myelopoiesis from Embryonic Stem Cells and Primary Hemopoietic Progenitors.
R. F. de Pooter, T. M. Schmitt, J. L. de la Pompa, Y. Fujiwara, S. H. Orkin, and J. C. Zuniga-Pflucker (2006)
J. Immunol. 176, 5267-5275
   Abstract »    Full Text »    PDF »
Thalidomide derivative CC-4047 inhibits osteoclast formation by down-regulation of PU.1.
G. Anderson, M. Gries, N. Kurihara, T. Honjo, J. Anderson, V. Donnenberg, A. Donnenberg, I. Ghobrial, M. Y. Mapara, D. Stirling, et al. (2006)
Blood 107, 3098-3105
   Abstract »    Full Text »    PDF »
Lineage specification and plasticity in CD19- early B cell precursors.
L. L. Rumfelt, Y. Zhou, B. M. Rowley, S. A. Shinton, and R. R. Hardy (2006)
J. Exp. Med. 203, 675-687
   Abstract »    Full Text »    PDF »
Multipotential differentiation ability of GATA-1-null erythroid-committed cells..
K. Kitajima, J. Zheng, H. Yen, D. Sugiyama, and T. Nakano (2006)
Genes & Dev. 20, 654-659
   Abstract »    Full Text »    PDF »
Epigenetic processes play a major role in B-cell-specific gene silencing in classical Hodgkin lymphoma.
A. Ushmorov, F. Leithauser, O. Sakk, A. Weinhausel, S. W. Popov, P. Moller, and T. Wirth (2006)
Blood 107, 2493-2500
   Abstract »    Full Text »    PDF »
The mechanism of repression of the myeloid-specific c-fms gene by Pax5 during B lineage restriction.
H. Tagoh, R. Ingram, N. Wilson, G. Salvagiotto, A. J. Warren, D. Clarke, M. Busslinger, and C. Bonifer (2006)
EMBO J. 25, 1070-1080
   Abstract »    Full Text »    PDF »
Redirecting differentiation of hematopoietic progenitors by a transcription factor, GATA-2.
K. Kitajima, M. Tanaka, J. Zheng, H. Yen, A. Sato, D. Sugiyama, H. Umehara, E. Sakai, and T. Nakano (2006)
Blood 107, 1857-1863
   Abstract »    Full Text »    PDF »
The ETS factor TEL2 is a hematopoietic oncoprotein.
C. Carella, M. Potter, J. Bonten, J. E. Rehg, G. Neale, and G. C. Grosveld (2006)
Blood 107, 1124-1132
   Abstract »    Full Text »    PDF »
Activation of the Flt3 signal transduction cascade rescues and enhances type I interferon-producing and dendritic cell development.
N. Onai, A. Obata-Onai, R. Tussiwand, A. Lanzavecchia, and M. G. Manz (2006)
J. Exp. Med. 203, 227-238
   Abstract »    Full Text »    PDF »
Hydrolytic and Nonenzymatic Functions of Acetylcholinesterase Comodulate Hemopoietic Stress Responses.
D. Grisaru, M. Pick, C. Perry, E. H. Sklan, R. Almog, I. Goldberg, E. Naparstek, J. B. Lessing, H. Soreq, and V. Deutsch (2006)
J. Immunol. 176, 27-35
   Abstract »    Full Text »    PDF »
Protein Acetylation Regulates Both PU.1 Transactivation and Ig{kappa} 3' Enhancer Activity.
Y. Bai, L. Srinivasan, L. Perkins, and M. L. Atchison (2005)
J. Immunol. 175, 5160-5169
   Abstract »    Full Text »    PDF »
PU.1 Regulates the Tissue-specific Expression of Dendritic Cell-specific Intercellular Adhesion Molecule (ICAM)-3-grabbing Nonintegrin.
A. Dominguez-Soto, A. Puig-Kroger, M. A. Vega, and A. L. Corbi (2005)
J. Biol. Chem. 280, 33123-33131
   Abstract »    Full Text »    PDF »
Identification of target genes and a unique cis element regulated by IRF-8 in developing macrophages.
T. Tamura, P. Thotakura, T. S. Tanaka, M. S. H. Ko, and K. Ozato (2005)
Blood 106, 1938-1947
   Abstract »    Full Text »    PDF »
The development of functional B lymphocytes in conditional PU.1 knock-out mice.
M. Polli, A. Dakic, A. Light, L. Wu, D. M. Tarlinton, and S. L. Nutt (2005)
Blood 106, 2083-2090
   Abstract »    Full Text »    PDF »
Dysplastic definitive hematopoiesis in AML1/EVI1 knock-in embryos.
K. Maki, T. Yamagata, T. Asai, I. Yamazaki, H. Oda, H. Hirai, and K. Mitani (2005)
Blood 106, 2147-2155
   Abstract »    Full Text »    PDF »
Knockdown of Pu.1 by small interfering RNA in CD34+ embryoid body cells derived from mouse ES cells turns cell fate determination to pro-B cells.
G.-M. Zou, J.-J. Chen, M. C. Yoder, W. Wu, and J. D. Rowley (2005)
PNAS 102, 13236-13241
   Abstract »    Full Text »    PDF »
Inducible binding of PU.1 and interacting proteins to the Toll-like receptor 4 promoter during endotoxemia.
T. V. Pedchenko, G. Y. Park, M. Joo, T. S. Blackwell, and J. W. Christman (2005)
Am J Physiol Lung Cell Mol Physiol 289, L429-L437
   Abstract »    Full Text »    PDF »
Effect of transcription-factor concentrations on leukemic stem cells.
F. Rosenbauer, S. Koschmieder, U. Steidl, and D. G. Tenen (2005)
Blood 106, 1519-1524
   Abstract »    Full Text »    PDF »
Distinctive and indispensable roles of PU.1 in maintenance of hematopoietic stem cells and their differentiation.
H. Iwasaki, C. Somoza, H. Shigematsu, E. A. Duprez, J. Iwasaki-Arai, S.-i. Mizuno, Y. Arinobu, K. Geary, P. Zhang, T. Dayaram, et al. (2005)
Blood 106, 1590-1600
   Abstract »    Full Text »    PDF »
Haploinsufficiency of GATA-2 perturbs adult hematopoietic stem-cell homeostasis.
N. P. Rodrigues, V. Janzen, R. Forkert, D. M. Dombkowski, A. S. Boyd, S. H. Orkin, T. Enver, P. Vyas, and D. T. Scadden (2005)
Blood 106, 477-484
   Abstract »    Full Text »    PDF »
Transcriptional Regulation of the Mouse IL-7 Receptor {alpha} Promoter by Glucocorticoid Receptor.
H.-C. Lee, H. Shibata, S. Ogawa, K. Maki, and K. Ikuta (2005)
J. Immunol. 174, 7800-7806
   Abstract »    Full Text »    PDF »
Enforced Expression of Spi-B Reverses T Lineage Commitment and Blocks {beta}-Selection.
J. M. Lefebvre, M. C. Haks, M. O. Carleton, M. Rhodes, G. Sinnathamby, M. C. Simon, L. C. Eisenlohr, L. A. Garrett-Sinha, and D. L. Wiest (2005)
J. Immunol. 174, 6184-6194
   Abstract »    Full Text »    PDF »
PU.1 regulates the commitment of adult hematopoietic progenitors and restricts granulopoiesis.
A. Dakic, D. Metcalf, L. Di Rago, S. Mifsud, L. Wu, and S. L. Nutt (2005)
J. Exp. Med. 201, 1487-1502
   Abstract »    Full Text »    PDF »
Contingent gene regulatory networks and B cell fate specification.
H. Singh, K. L. Medina, and J. M. R. Pongubala (2005)
PNAS 102, 4949-4953
   Abstract »    Full Text »    PDF »
Balance of MafB and PU.1 specifies alternative macrophage or dendritic cell fate.
Y. Bakri, S. Sarrazin, U. P. Mayer, S. Tillmanns, C. Nerlov, A. Boned, and M. H. Sieweke (2005)
Blood 105, 2707-2716
   Abstract »    Full Text »    PDF »
Formation of an Active Tissue-Specific Chromatin Domain Initiated by Epigenetic Marking at the Embryonic Stem Cell Stage.
H. Szutorisz, C. Canzonetta, A. Georgiou, C.-M. Chow, L. Tora, and N. Dillon (2005)
Mol. Cell. Biol. 25, 1804-1820
   Abstract »    Full Text »    PDF »
Dynamic regulation of PU.1 expression in multipotent hematopoietic progenitors.
S. L. Nutt, D. Metcalf, A. D'Amico, M. Polli, and L. Wu (2005)
J. Exp. Med. 201, 221-231
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Mast Cells Acquire Monocyte-Specific Gene Expression and Monocyte-Like Morphology by Overproduction of PU.1.
T. Ito, C. Nishiyama, M. Nishiyama, H. Matsuda, K. Maeda, Y. Akizawa, R. Tsuboi, K. Okumura, and H. Ogawa (2005)
J. Immunol. 174, 376-383
   Abstract »    Full Text »    PDF »
The ETS Transcription Factor Spi-B Is Required for Human Plasmacytoid Dendritic Cell Development.
R. Schotte, M. Nagasawa, K. Weijer, H. Spits, and B. Blom (2004)
J. Exp. Med. 200, 1503-1509
   Abstract »    Full Text »    PDF »
PU.1 is a suppressor of myeloid leukemia, inactivated in mice by gene deletion and mutation of its DNA binding domain.
W. D. Cook, B. J. McCaw, C. Herring, D. L. John, S. J. Foote, S. L. Nutt, and J. M. Adams (2004)
Blood 104, 3437-3444
   Abstract »    Full Text »    PDF »
Epigenetic silencing of the c-fms locus during B-lymphopoiesis occurs in discrete steps and is reversible.
H. Tagoh, A. Schebesta, P. Lefevre, N. Wilson, D. Hume, M. Busslinger, and C. Bonifer (2004)
EMBO J. 23, 4275-4285
   Abstract »    Full Text »    PDF »
Form follows function: the genomic organization of cellular differentiation.
S. T. Kosak and M. Groudine (2004)
Genes & Dev. 18, 1371-1384
   Abstract »    Full Text »    PDF »
The roles of transcription factors in B lymphocyte commitment, development, and transformation.
E. Smith and M. Sigvardsson (2004)
J. Leukoc. Biol. 75, 973-981
   Abstract »    Full Text »    PDF »
The interferon regulatory factor ICSBP/IRF-8 in combination with PU.1 up-regulates expression of tumor suppressor p15Ink4b in murine myeloid cells.
M. Schmidt, J. Bies, T. Tamura, K. Ozato, and L. Wolff (2004)
Blood 103, 4142-4149
   Abstract »    Full Text »    PDF »
pDP4, a novel glycoprotein secreted by mature granulocytes, is regulated by transcription factor PU.1.
F. Rosenbauer, K. Wagner, P. Zhang, K.-P. Knobeloch, A. Iwama, and D. G. Tenen (2004)
Blood 103, 4294-4301
   Abstract »    Full Text »    PDF »
PU.1 determines the self-renewal capacity of erythroid progenitor cells.
J. Back, A. Dierich, C. Bronn, P. Kastner, and S. Chan (2004)
Blood 103, 3615-3623
   Abstract »    Full Text »    PDF »
Transcriptional Regulation of the Cyclooxygenase-2 Gene in Macrophages by PU.1.
M. Joo, G. Y. Park, J. G. Wright, T. S. Blackwell, M. L. Atchison, and J. W. Christman (2004)
J. Biol. Chem. 279, 6658-6665
   Abstract »    Full Text »    PDF »

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