Yikang S. Rong, Kent G. Golic ^*

Drosophila offers many advantages as an experimental organism. However, in comparison with yeast and mouse, two other widely used eukaryotic model systems, Drosophila suffers from an inability to perform homologous recombination between introduced DNA and the corresponding chromosomal loci. The ability to specifically modify the genomes of yeast and mouse provides a quick and easy way to generate or rescue mutations in genes for which a DNA clone or sequence is available. A method is described that enables analogous manipulations of the Drosophila genome. This technique may also be applicable to other organisms for which gene-targeting procedures do not yet exist.

Department of Biology, University of Utah, Salt Lake City, UT 84112, USA.
^*   To whom correspondence should be addressed. E-mail: golic{at}bioscience.utah.edu

Atg6 is required for multiple vesicle trafficking pathways and hematopoiesis in Drosophila.

B. V. Shravage, J. H. Hill, C. M. Powers, L. Wu, and E. H. Baehrecke (2013)
Development 140, 1321-1329
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Mutations to the piRNA Pathway Component Aubergine Enhance Meiotic Drive of Segregation Distorter in Drosophila melanogaster.

S. L. Gell and R. A. Reenan (2013)
Genetics 193, 771-784
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Targeted Gene Replacement in Drosophila Goes the Distance.

K. N. Crown and J. Sekelsky (2013)
Genetics 193, 377-381
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Captured Segment Exchange: A Strategy for Custom Engineering Large Genomic Regions in Drosophila melanogaster.

J. R. Bateman, M. F. Palopoli, S. T. Dale, J. E. Stauffer, A. L. Shah, J. E. Johnson, C. W. Walsh, H. Flaten, and C. M. Parsons (2013)
Genetics 193, 421-430
 |  Abstract »  |  Full Text »  |  PDF »

Long-Range Targeted Manipulation of the Drosophila Genome by Site-Specific Integration and Recombinational Resolution.

N. Wesolowska and Y. S. Rong (2013)
Genetics 193, 411-419
 |  Abstract »  |  Full Text »  |  PDF »

Drosophila as a Model to Study Mitochondrial Dysfunction in Parkinson's Disease.

M. Guo (2012)
Cold Spring Harb Perspect Med 2, a009944
 |  Abstract »  |  Full Text »  |  PDF »

A Knock-In Model of Human Epilepsy in Drosophila Reveals a Novel Cellular Mechanism Associated with Heat-Induced Seizure.

L. Sun, J. Gilligan, C. Staber, R. J. Schutte, V. Nguyen, D. K. O'Dowd, and R. Reenan (2012)
J. Neurosci. 32, 14145-14155
 |  Abstract »  |  Full Text »  |  PDF »

Molecular Genetic Analysis of Sexual Rejection: Roles of Octopamine and Its Receptor OAMB in Drosophila Courtship Conditioning.

C. Zhou, H. Huang, S. M. Kim, H. Lin, X. Meng, K.-A. Han, A.-S. Chiang, J. W. Wang, R. Jiao, and Y. Rao (2012)
J. Neurosci. 32, 14281-14287
 |  Abstract »  |  Full Text »  |  PDF »

Type-I Prenyl Protease Function Is Required in the Male Germline of Drosophila melanogaster.

K. Adolphsen, A. Amell, N. Havko, S. Kevorkian, K. Mears, H. Neher, D. Schwarz, and S. R. Schulze (2012)
g3 2, 629-642
 |  Abstract »  |  Full Text »  |  PDF »

In planta gene targeting.

F. Fauser, N. Roth, M. Pacher, G. Ilg, R. Sanchez-Fernandez, C. Biesgen, and H. Puchta (2012)
PNAS 109, 7535-7540
 |  Abstract »  |  Full Text »  |  PDF »

A Novel Approach for Directing Transgene Expression in Drosophila: T2A-Gal4 In-Frame Fusion.

F. Diao and B. H. White (2012)
Genetics 190, 1139-1144
 |  Abstract »  |  Full Text »  |  PDF »

Drosophila, Genetic Screens, and Cardiac Function.

M. J. Wolf and H. A. Rockman (2011)
Circ. Res. 109, 794-806
 |  Abstract »  |  Full Text »  |  PDF »

The seven-pass transmembrane cadherin Flamingo controls dendritic self-avoidance via its binding to a LIM domain protein, Espinas, in Drosophila sensory neurons.

D. Matsubara, S.-y. Horiuchi, K. Shimono, T. Usui, and T. Uemura (2011)
Genes & Dev. 25, 1982-1996
 |  Abstract »  |  Full Text »  |  PDF »

mir-11 limits the proapoptotic function of its host gene, dE2f1.

M. Truscott, A. B. M. M. K. Islam, N. Lopez-Bigas, and M. V. Frolov (2011)
Genes & Dev. 25, 1820-1834
 |  Abstract »  |  Full Text »  |  PDF »

Insect Population Control by Homing Endonuclease-Based Gene Drive: An Evaluation in Drosophila melanogaster.

Y.-S. Chan, D. A. Naujoks, D. S. Huen, and S. Russell (2011)
Genetics 188, 33-44
 |  Abstract »  |  Full Text »  |  PDF »

Engineered Alterations in RNA Editing Modulate Complex Behavior in Drosophila: REGULATORY DIVERSITY OF ADENOSINE DEAMINASE ACTING ON RNA (ADAR) TARGETS.

J. E. C. Jepson, Y. A. Savva, C. Yokose, A. U. Sugden, A. Sahin, and R. A. Reenan (2011)
J. Biol. Chem. 286, 8325-8337
 |  Abstract »  |  Full Text »  |  PDF »

The I-CreI meganuclease and its engineered derivatives: applications from cell modification to gene therapy.

S. Arnould, C. Delenda, S. Grizot, C. Desseaux, F. Paques, G. H. Silva, and J. Smith (2011)
Protein Eng. Des. Sel. 24, 27-31
 |  Abstract »  |  Full Text »  |  PDF »

Drosophila provides rapid modeling of renal development, function, and disease.

J. A. T. Dow and M. F. Romero (2010)
Am J Physiol Renal Physiol 299, F1237-F1244
 |  Abstract »  |  Full Text »  |  PDF »

Drosophila von Hippel-Lindau Tumor Suppressor Gene Function in Epithelial Tubule Morphogenesis.

A. Hsouna, G. Nallamothu, N. Kose, M. Guinea, V. Dammai, and T. Hsu (2010)
Mol. Cell. Biol. 30, 3779-3794
 |  Abstract »  |  Full Text »  |  PDF »

Molecular Genetic Analysis of Chd3 and Polytene Chromosome Region 76B-D in Drosophila melanogaster.

M. T. Cooper, A. W. Conant, and J. A. Kennison (2010)
Genetics 185, 811-822
 |  Abstract »  |  Full Text »  |  PDF »

Drosophila Histone Deacetylase 6 Protects Dopaminergic Neurons against {alpha}-Synuclein Toxicity by Promoting Inclusion Formation.

G. Du, X. Liu, X. Chen, M. Song, Y. Yan, R. Jiao, and C.-c. Wang (2010)
Mol. Biol. Cell 21, 2128-2137
 |  Abstract »  |  Full Text »  |  PDF »

Sialyltransferase Regulates Nervous System Function in Drosophila.

E. Repnikova, K. Koles, M. Nakamura, J. Pitts, H. Li, A. Ambavane, M. J. Zoran, and V. M. Panin (2010)
J. Neurosci. 30, 6466-6476
 |  Abstract »  |  Full Text »  |  PDF »

Drosophila VHL tumor-suppressor gene regulates epithelial morphogenesis by promoting microtubule and aPKC stability.

S. Duchi, L. Fagnocchi, V. Cavaliere, A. Hsouna, G. Gargiulo, and T. Hsu (2010)
Development 137, 1493-1503
 |  Abstract »  |  Full Text »  |  PDF »

Recombinase-Mediated Cassette Exchange Provides a Versatile Platform for Gene Targeting: Knockout of miR-31b.

R. Weng, Y.-W. Chen, N. Bushati, A. Cliffe, and S. M. Cohen (2009)
Genetics 183, 399-402
 |  Abstract »  |  Full Text »  |  PDF »

Large-Scale Functional Annotation and Expanded Implementations of the P{wHy} Hybrid Transposon in the Drosophila melanogaster Genome.

K. V. Myrick, F. Huet, S. E. Mohr, I. Alvarez-Garcia, J. T. Lu, M. A. Smith, M. A. Crosby, and W. M. Gelbart (2009)
Genetics 182, 653-660
 |  Abstract »  |  Full Text »  |  PDF »

Genetic Analysis of Zinc-Finger Nuclease-Induced Gene Targeting in Drosophila.

A. Bozas, K. J. Beumer, J. K. Trautman, and D. Carroll (2009)
Genetics 182, 641-651
 |  Abstract »  |  Full Text »  |  PDF »

SIRT Combines Homologous Recombination, Site-Specific Integration, and Bacterial Recombineering for Targeted Mutagenesis in Drosophila.

G. Gao, N. Wesolowska, and Y. S. Rong (2009)
Cold Spring Harb Protoc 2009, pdb.prot5236
 |  Abstract »  |  Full Text »  |  PDF »

Directed, efficient, and versatile modifications of the Drosophila genome by genomic engineering.

J. Huang, W. Zhou, W. Dong, A. M. Watson, and Y. Hong (2009)
PNAS 106, 8284-8289
 |  Abstract »  |  Full Text »  |  PDF »

Spatiotemporal control of mitosis by the conserved spindle matrix protein Megator.

M. Lince-Faria, S. Maffini, B. Orr, Y. Ding, Claudia Florindo, C. E. Sunkel, A. Tavares, J. Johansen, K. M. Johansen, and H. Maiato (2009)
J. Cell Biol. 184, 647-657
 |  Abstract »  |  Full Text »  |  PDF »

The 2009 Novitski Prize.

J. E. Haber (2009)
Genetics 181, 837-838
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Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases.

K. J. Beumer, J. K. Trautman, A. Bozas, J.-L. Liu, J. Rutter, J. G. Gall, and D. Carroll (2008)
PNAS 105, 19821-19826
 |  Abstract »  |  Full Text »  |  PDF »

Distinct TRP channels are required for warm and cool avoidance in Drosophila melanogaster.

M. Rosenzweig, K. Kang, and P. A. Garrity (2008)
PNAS 105, 14668-14673
 |  Abstract »  |  Full Text »  |  PDF »

A powerful method combining homologous recombination and site-specific recombination for targeted mutagenesis in Drosophila.

G. Gao, C. McMahon, J. Chen, and Y. S. Rong (2008)
PNAS 105, 13999-14004
 |  Abstract »  |  Full Text »  |  PDF »

Efficient Ends-Out Gene Targeting In Drosophila.

J. Huang, W. Zhou, A. M. Watson, Y.-N. Jan, and Y. Hong (2008)
Genetics 180, 703-707
 |  Abstract »  |  Full Text »  |  PDF »

The origin recognition complex is dispensable for endoreplication in Drosophila.

S. Y. Park and M. Asano (2008)
PNAS 105, 12343-12348
 |  Abstract »  |  Full Text »  |  PDF »

The YPWM motif links Antennapedia to the basal transcriptional machinery.

F. Prince, T. Katsuyama, Y. Oshima, S. Plaza, D. Resendez-Perez, M. Berry, S. Kurata, and W. J. Gehring (2008)
Development 135, 1669-1679
 |  Abstract »  |  Full Text »  |  PDF »

Targeted Gene Deletion and Phenotypic Analysis of the Drosophila melanogaster Seminal Fluid Protease Inhibitor Acp62F.

J. L. Mueller, J. R. Linklater, K. Ravi Ram, T. Chapman, and M. F. Wolfner (2008)
Genetics 178, 1605-1614
 |  Abstract »  |  Full Text »  |  PDF »

Transgenesis upgrades for Drosophila melanogaster.

K. J. T. Venken and H. J. Bellen (2007)
Development 134, 3571-3584
 |  Abstract »  |  Full Text »  |  PDF »

Homing endonuclease mediated gene targeting in Anopheles gambiae cells and embryos.

N. Windbichler, P. A. Papathanos, F. Catteruccia, H. Ranson, A. Burt, and A. Crisanti (2007)
Nucleic Acids Res. 35, 5922-5933
 |  Abstract »  |  Full Text »  |  PDF »

A Genetic Screen For DNA Double-Strand Break Repair Mutations in Drosophila.

D. S. Wei and Y. S. Rong (2007)
Genetics 177, 63-77
 |  Abstract »  |  Full Text »  |  PDF »

Rhythm Defects Caused by Newly Engineered Null Mutations in Drosophila's cryptochrome Gene.

E. Dolezelova, D. Dolezel, and J. C. Hall (2007)
Genetics 177, 329-345
 |  Abstract »  |  Full Text »  |  PDF »

Synthetic Lethality of Drosophila in the Absence of the MUS81 Endonuclease and the DmBlm Helicase Is Associated With Elevated Apoptosis.

K. Trowbridge, K. McKim, S. J. Brill, and J. Sekelsky (2007)
Genetics 176, 1993-2001
 |  Abstract »  |  Full Text »  |  PDF »

Characterization of BEAF Mutations Isolated by Homologous Recombination in Drosophila.

S. Roy, M. K. Gilbert, and C. M. Hart (2007)
Genetics 176, 801-813
 |  Abstract »  |  Full Text »  |  PDF »

Integrative physiology, functional genomics and the phenotype gap: a guide for comparative physiologists.

J. A. T. Dow (2007)
J. Exp. Biol. 210, 1632-1640
 |  Abstract »  |  Full Text »  |  PDF »

In Vivo Construction of Transgenes in Drosophila.

H. Takeuchi, O. Georgiev, M. Fetchko, M. Kappeler, W. Schaffner, and D. Egli (2007)
Genetics 175, 2019-2028
 |  Abstract »  |  Full Text »  |  PDF »

Drosophila ATR in Double-Strand Break Repair.

J. R. LaRocque, B. Jaklevic, T. T. Su, and J. Sekelsky (2007)
Genetics 175, 1023-1033
 |  Abstract »  |  Full Text »  |  PDF »

Homing endonuclease I-CreI derivatives with novel DNA target specificities.

L. E. Rosen, H. A. Morrison, S. Masri, M. J. Brown, B. Springstubb, D. Sussman, B. L. Stoddard, and L. M. Seligman (2006)
Nucleic Acids Res. 34, 4791-4800
 |  Abstract »  |  Full Text »  |  PDF »

Transcriptome response to heavy metal stress in Drosophila reveals a new zinc transporter that confers resistance to zinc.

H. Yepiskoposyan, D. Egli, T. Fergestad, A. Selvaraj, C. Treiber, G. Multhaup, O. Georgiev, and W. Schaffner (2006)
Nucleic Acids Res. 34, 4866-4877
 |  Abstract »  |  Full Text »  |  PDF »

Site-Specific Transformation of Drosophila via {phi}C31 Integrase-Mediated Cassette Exchange.

J. R. Bateman, A. M. Lee, and C.-t. Wu (2006)
Genetics 173, 769-777
 |  Abstract »  |  Full Text »  |  PDF »

Molecular Genetics of Natural Populations.

A. J. Greenberg and C.-I Wu (2006)
Mol. Biol. Evol. 23, 883-886
 |  Abstract »  |  Full Text »  |  PDF »

Efficient Gene Targeting in Drosophila With Zinc-Finger Nucleases.

K. Beumer, G. Bhattacharyya, M. Bibikova, J. K. Trautman, and D. Carroll (2006)
Genetics 172, 2391-2403
 |  Abstract »  |  Full Text »  |  PDF »

Molecular Characterization of True and Ectopic Gene Targeting Events at the Acetolactate Synthase Gene in Arabidopsis.

M. Endo, K. Osakabe, H. Ichikawa, and S. Toki (2006)
Plant Cell Physiol. 47, 372-379
 |  Abstract »  |  Full Text »  |  PDF »

Pleiotropic functions of a conserved insect-specific Hox peptide motif.

C. T. Hittinger, D. L. Stern, and S. B. Carroll (2005)
Development 132, 5261-5270
 |  Abstract »  |  Full Text »  |  PDF »

In vivo selection of engineered homing endonucleases using double-strand break induced homologous recombination.

P. Chames, J.-C. Epinat, S. Guillier, A. Patin, E. Lacroix, and F. Paques (2005)
Nucleic Acids Res. 33, e178
 |  Abstract »  |  Full Text »  |  PDF »

Highly Efficient Sex Chromosome Interchanges Produced By I-CreI Expression in Drosophila.

K. A. Maggert and K. G. Golic (2005)
Genetics 171, 1103-1114
 |  Abstract »  |  Full Text »  |  PDF »

Mesodermally expressed Drosophila microRNA-1 is regulated by Twist and is required in muscles during larval growth.

N. S. Sokol and V. Ambros (2005)
Genes & Dev. 19, 2343-2354
 |  Abstract »  |  Full Text »  |  PDF »

Site-specific genomic targeting in Drosophila.

C. Horn and A. M. Handler (2005)
PNAS 102, 12483-12488
 |  Abstract »  |  Full Text »  |  PDF »

High-frequency gene targeting in Arabidopsis plants expressing the yeast RAD54 gene.

H. Shaked, C. Melamed-Bessudo, and A. A. Levy (2005)
PNAS 102, 12265-12269
 |  Abstract »  |  Full Text »  |  PDF »

Drosophila ERCC1 Is Required for a Subset of MEI-9-Dependent Meiotic Crossovers.

S. J. Radford, E. Goley, K. Baxter, S. McMahan, and J. Sekelsky (2005)
Genetics 170, 1737-1745
 |  Abstract »  |  Full Text »  |  PDF »

I-ApeI: a novel intron-encoded LAGLIDADG homing endonuclease from the archaeon, Aeropyrum pernix K1.

N. Nomura, Y. Morinaga, N. Shirai, and Y. Sako (2005)
Nucleic Acids Res. 33, e116
 |  Abstract »  |  Full Text »  |  PDF »

Regulation of phototransduction responsiveness and retinal degeneration by a phospholipase D-generated signaling lipid.

M. M. LaLonde, H. Janssens, E. Rosenbaum, S.-Y. Choi, J. P. Gergen, N. J. Colley, W. S. Stark, and M. A. Frohman (2005)
J. Cell Biol. 169, 471-479
 |  Abstract »  |  Full Text »  |  PDF »

The repair of double-strand breaks in plants: mechanisms and consequences for genome evolution.

H. Puchta (2005)
J. Exp. Bot. 56, 1-14
 |  Abstract »  |  Full Text »  |  PDF »

Genomic Deletions of the Drosophila melanogaster Hsp70 Genes.

W. J. Gong and K. G. Golic (2004)
Genetics 168, 1467-1476
 |  Abstract »  |  Full Text »  |  PDF »

Gene Deletions by Ends-In Targeting in Drosophila melanogaster.

H. B. Xie and K. G. Golic (2004)
Genetics 168, 1477-1489
 |  Abstract »  |  Full Text »  |  PDF »

Targeted Disruption of Drosophila Roc1b Reveals Functional Differences in the Roc Subunit of Cullin-dependent E3 Ubiquitin Ligases.

T. D. Donaldson, M. A. Noureddine, P. J. Reynolds, W. Bradford, and R. J. Duronio (2004)
Mol. Biol. Cell 15, 4892-4903
 |  Abstract »  |  Full Text »  |  PDF »

Functional Analysis of Drosophila melanogaster Gene Regulatory Sequences by Transgene Coplacement.

J. Parsch (2004)
Genetics 168, 559-561
 |  Abstract »  |  Full Text »  |  PDF »

Dm-myb mutant lethality in Drosophila is dependent upon mip130: positive and negative regulation of DNA replication.

E. L. Beall, M. Bell, D. Georlette, and M. R. Botchan (2004)
Genes & Dev. 18, 1667-1680
 |  Abstract »  |  Full Text »  |  PDF »

The Normal Function of a Speciation Gene, Odysseus, and Its Hybrid Sterility Effect.

S. Sun, C.-T. Ting, and C.-I Wu (2004)
Science 305, 81-83
 |  Abstract »  |  Full Text »  |  PDF »

An Efficient Method to Generate Chromosomal Rearrangements by Targeted DNA Double-Strand Breaks in Drosophila melanogaster.

D. Egli, E. Hafen, and W. Schaffner (2004)
Genome Res. 14, 1382-1393
 |  Abstract »  |  Full Text »  |  PDF »

Construction of Transgenic Drosophila by Using the Site-Specific Integrase From Phage {phi}C31.

A. C. Groth, M. Fish, R. Nusse, and M. P. Calos (2004)
Genetics 166, 1775-1782
 |  Abstract »  |  Full Text »  |  PDF »

Homologous gene targeting in Caenorhabditis elegans by biolistic transformation.

E. Berezikov, C. I. Bargmann, and R. H. A. Plasterk (2004)
Nucleic Acids Res. 32, e40
 |  Abstract »  |  Full Text »  |  PDF »

The Homologous Chromosome Is an Effective Template for the Repair of Mitotic DNA Double-Strand Breaks in Drosophila.

Y. S. Rong and K. G. Golic (2003)
Genetics 165, 1831-1842
 |  Abstract »  |  Full Text »  |  PDF »

The Drosophila melanogaster DNA Ligase IV Gene Plays a Crucial Role in the Repair of Radiation-Induced DNA Double-Strand Breaks and Acts Synergistically With Rad54.

M. M. Gorski, J. C. J. Eeken, A. W. M. de Jong, I. Klink, M. Loos, R. J. Romeijn, B. L. van Veen, L. H. Mullenders, W. Ferro, and A. Pastink (2003)
Genetics 165, 1929-1941
 |  Abstract »  |  Full Text »  |  PDF »

High efficiency, site-specific excision of a marker gene by the phage P1 cre-loxP system in the yellow fever mosquito, Aedes aegypti.

N. Jasinskiene, C. J. Coates, A. Ashikyan, and A. A. James (2003)
Nucleic Acids Res. 31, e147
 |  Abstract »  |  Full Text »  |  PDF »

Targeted Mutation of a Drosophila Odor Receptor Defines Receptor Requirement in a Novel Class of Sensillum.

T. Elmore, R. Ignell, J. R. Carlson, and D. P. Smith (2003)
J. Neurosci. 23, 9906-9912
 |  Abstract »  |  Full Text »  |  PDF »

Genetic Analysis of the ADGF Multigene Family by Homologous Recombination and Gene Conversion in Drosophila.

T. Dolezal, M. Gazi, M. Zurovec, and P. J. Bryant (2003)
Genetics 165, 653-666
 |  Abstract »  |  Full Text »  |  PDF »

Sex-peptide is the molecular basis of the sperm effect in Drosophila melanogaster.

H. Liu and E. Kubli (2003)
PNAS 100, 9929-9933
 |  Abstract »  |  Full Text »  |  PDF »

Sex peptide and the sperm effect in Drosophila melanogaster.

W. J. Swanson (2003)
PNAS 100, 9643-9644
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Macrophage-mediated corpse engulfment is required for normal Drosophila CNS morphogenesis.

H. C. Sears, C. J. Kennedy, and P. A. Garrity (2003)
Development 130, 3557-3565
 |  Abstract »  |  Full Text »  |  PDF »

A novel engineered meganuclease induces homologous recombination in yeast and mammalian cells.

J.-C. Epinat, S. Arnould, P. Chames, P. Rochaix, D. Desfontaines, C. Puzin, A. Patin, A. Zanghellini, F. Paques, and E. Lacroix (2003)
Nucleic Acids Res. 31, 2952-2962
 |  Abstract »  |  Full Text »  |  PDF »

Enhancing Gene Targeting with Designed Zinc Finger Nucleases.

M. Bibikova, K. Beumer, J. K. Trautman, and D. Carroll (2003)
Science 300, 764
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Drosophila p53 preserves genomic stability by regulating cell death.

N. Sogame, M. Kim, and J. M. Abrams (2003)
PNAS 100, 4696-4701
 |  Abstract »  |  Full Text »  |  PDF »

Ends-out, or replacement, gene targeting in Drosophila.

W. J. Gong and K. G. Golic (2003)
PNAS 100, 2556-2561
 |  Abstract »  |  Full Text »  |  PDF »

Discovery of genes with highly restricted expression patterns in the Drosophila wing disc using DNA oligonucleotide microarrays.

M. J. Butler, T. L. Jacobsen, D. M. Cain, M. G. Jarman, M. Hubank, J. R. S. Whittle, R. Phillips, and A. Simcox (2003)
Development 130, 659-670
 |  Abstract »  |  Full Text »  |  PDF »

Knockout Targeting of the Drosophila Nap1 Gene and Examination of DNA Repair Tracts in the Recombination Products.

S. Lankenau, T. Barnickel, J. Marhold, F. Lyko, B. M. Mechler, and D.-H. Lankenau (2003)
Genetics 163, 611-623
 |  Abstract »  |  Full Text »  |  PDF »

Mutations altering the cleavage specificity of a homing endonuclease.

L. M. Seligman, K. M. Chisholm, B. S. Chevalier, M. S. Chadsey, S. T. Edwards, J. H. Savage, and A. L. Veillet (2002)
Nucleic Acids Res. 30, 3870-3879
 |  Abstract »  |  Full Text »  |  PDF »

Using the P{wHy} Hybrid Transposable Element to Disrupt Genes in Region 54D-55B in Drosophila melanogaster.

S. E. Mohr and W. M. Gelbart (2002)
Genetics 162, 165-176
 |  Abstract »  |  Full Text »  |  PDF »

A deletion-generator compound element allows deletion saturation analysis for genomewide phenotypic annotation.

F. Huet, J. T. Lu, K. V. Myrick, L. R. Baugh, M. A. Crosby, and W. M. Gelbart (2002)
PNAS 99, 9948-9953
 |  Abstract »  |  Full Text »  |  PDF »

Isolation of Su(var)3-7 Mutations by Homologous Recombination in Drosophila melanogaster.

C. Seum, D. Pauli, M. Delattre, Y. Jaquet, A. Spierer, and P. Spierer (2002)
Genetics 161, 1125-1136
 |  Abstract »  |  Full Text »  |  PDF »

Targeted Chromosomal Cleavage and Mutagenesis in Drosophila Using Zinc-Finger Nucleases.

M. Bibikova, M. Golic, K. G. Golic, and D. Carroll (2002)
Genetics 161, 1169-1175
 |  Abstract »  |  Full Text »  |  PDF »

Targeted mutagenesis by homologous recombination in D. melanogaster.

Y. S. Rong, S. W. Titen, H. B. Xie, M. M. Golic, M. Bastiani, P. Bandyopadhyay, B. M. Olivera, M. Brodsky, G. M. Rubin, and K. G. Golic (2002)
Genes & Dev. 16, 1568-1581
 |  Abstract »  |  Full Text »  |  PDF »

Efficient Repair of DNA Breaks in Drosophila: Evidence for Single-Strand Annealing and Competition With Other Repair Pathways.

C. R. Preston, W. Engels, and C. Flores (2002)
Genetics 161, 711-720
 |  Abstract »  |  Full Text »  |  PDF »

Timing and Targeting of P-Element Local Transposition in the Male Germline Cells of Drosophila melanogaster.

B. Timakov, X. Liu, I. Turgut, and P. Zhang (2002)
Genetics 160, 1011-1022
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Analysis of Conserved Noncoding DNA in Drosophila Reveals Similar Constraints in Intergenic and Intronic Sequences.

C. M. Bergman and M. Kreitman (2001)
Genome Res. 11, 1335-1345
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The Drosophila Homolog of Mammalian Zinc Finger Factor MTF-1 Activates Transcription in Response to Heavy Metals.

B. Zhang, D. Egli, O. Georgiev, and W. Schaffner (2001)
Mol. Cell. Biol. 21, 4505-4514
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Evolutionary EST analysis identifies rapidly evolving male reproductive proteins in Drosophila.

W. J. Swanson, A. G. Clark, H. M. Waldrip-Dail, M. F. Wolfner, and C. F. Aquadro (2001)
PNAS 98, 7375-7379
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