Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Subscribe

Logo for

Science 290 (5492): 801-806

Copyright © 2000 by the American Association for the Advancement of Science

Integration of Multiple Signals Through Cooperative Regulation of the N-WASP-Arp2/3 Complex

Kenneth E. Prehoda,12 Jessica A. Scott,12 R. Dyche Mullins,1 Wendell A. Lim12*

The protein N-WASP [a homolog to the Wiskott-Aldrich syndrome protein (WASP)] regulates actin polymerization by stimulating the actin-nucleating activity of the actin-related protein 2/3 (Arp2/3) complex. N-WASP is tightly regulated by multiple signals: Only costimulation by Cdc42 and phosphatidylinositol (4,5)-bisphosphate (PIP2) yields potent polymerization. We found that regulation requires N-WASP's constitutively active output domain (VCA) and two regulatory domains: a Cdc42-binding domain and a previously undescribed PIP2-binding domain. In the absence of stimuli, the regulatory modules together hold the VCA-Arp2/3 complex in an inactive "closed" conformation. In this state, both the Cdc42- and PIP2-binding sites are masked. Binding of either input destabilizes the closed state and enhances binding of the other input. This cooperative activation mechanism shows how combinations of simple binding domains can be used to integrate and amplify coincident signals.

1 Department of Cellular and Molecular Pharmacology, and
2 Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143-0450, USA.
*   To whom correspondence should be addressed. E-mail: wlim{at}itsa.ucsf.edu

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
STAT3-mediated Coincidence Detection Regulates Noncanonical Immediate Early Gene Induction.
M. S. Waitkus, U. M. Chandrasekharan, B. Willard, S. J. Haque, and P. E. DiCorleto (2013)
J. Biol. Chem. 288, 11988-12003
   Abstract »    Full Text »    PDF »
Synergistic Activation of p21-activated Kinase 1 by Phosphatidylinositol 4,5-Bisphosphate and Rho GTPases.
K. A. Malecka, Z. Szentpetery, and J. R. Peterson (2013)
J. Biol. Chem. 288, 8887-8897
   Abstract »    Full Text »    PDF »
Phosphorylation of Actin-related Protein 2 (Arp2) Is Required for Normal Development and cAMP Chemotaxis in Dictyostelium.
C.-H. Choi, P. A. Thomason, M. Zaki, R. H. Insall, and D. L. Barber (2013)
J. Biol. Chem. 288, 2464-2474
   Abstract »    Full Text »    PDF »
Disease-associated missense mutations in the EVH1 domain disrupt intrinsic WASp function causing dysregulated actin dynamics and impaired dendritic cell migration.
A. J. J. Worth, J. Metelo, G. Bouma, D. Moulding, M. Fritzsche, B. Vernay, G. Charras, G. O. C. Cory, A. J. Thrasher, and S. O. Burns (2013)
Blood 121, 72-84
   Abstract »    Full Text »    PDF »
Focal adhesion kinase regulates actin nucleation and neuronal filopodia formation during axonal growth.
M. R. Chacon, A. I. Navarro, G. Cuesto, I. del Pino, R. Scott, M. Morales, and B. Rico (2012)
Development 139, 3200-3210
   Abstract »    Full Text »    PDF »
Activity-Dependent Fusion Pore Expansion Regulated by a Calcineurin-Dependent Dynamin-Syndapin Pathway in Mouse Adrenal Chromaffin Cells.
P. Samasilp, S.-A. Chan, and C. Smith (2012)
J. Neurosci. 32, 10438-10447
   Abstract »    Full Text »    PDF »
Nck and Cdc42 co-operate to recruit N-WASP to promote Fc{gamma}R-mediated phagocytosis.
A. E. Dart, S. K. Donnelly, D. W. Holden, M. Way, and E. Caron (2012)
J. Cell Sci. 125, 2825-2830
   Abstract »    Full Text »    PDF »
Stoichiometry of Nck-dependent actin polymerization in living cells.
J. A. Ditlev, P. J. Michalski, G. Huber, G. M. Rivera, W. A. Mohler, L. M. Loew, and B. J. Mayer (2012)
J. Cell Biol. 197, 643-658
   Abstract »    Full Text »    PDF »
WIP Is a Negative Regulator of Neuronal Maturation and Synaptic Activity.
A. Franco, S. Knafo, I. Banon-Rodriguez, P. Merino-Serrais, I. Fernaud-Espinosa, M. Nieto, J. J. Garrido, J. A. Esteban, F. Wandosell, and I. M. Anton (2012)
Cereb Cortex 22, 1191-1202
   Abstract »    Full Text »    PDF »
Dyrk1A negatively regulates the actin cytoskeleton through threonine phosphorylation of N-WASP.
J. Park, J. Y. Sung, J. Park, W.-J. Song, S. Chang, and K. C. Chung (2012)
J. Cell Sci. 125, 67-80
   Abstract »    Full Text »    PDF »
The actin nucleation factor JMY is a negative regulator of neuritogenesis.
E. N. Firat-Karalar, P. P. Hsiue, and M. D. Welch (2011)
Mol. Biol. Cell 22, 4563-4574
   Abstract »    Full Text »    PDF »
A Novel Phospholipase D2-Grb2-WASp Heterotrimer Regulates Leukocyte Phagocytosis in a Two-Step Mechanism.
S. Kantonen, N. Hatton, M. Mahankali, K. M. Henkels, H. Park, D. Cox, and J. Gomez-Cambronero (2011)
Mol. Cell. Biol. 31, 4524-4537
   Abstract »    Full Text »    PDF »
Mechanism and cellular function of Bud6 as an actin nucleation-promoting factor.
B. R. Graziano, A. G. DuPage, A. Michelot, D. Breitsprecher, J. B. Moseley, I. Sagot, L. Blanchoin, and B. L. Goode (2011)
Mol. Biol. Cell 22, 4016-4028
   Abstract »    Full Text »    PDF »
Phosphoinositides in Cell Architecture.
A. Shewan, D. J. Eastburn, and K. Mostov (2011)
Cold Spring Harb Perspect Biol 3, a004796
   Abstract »    Full Text »    PDF »
Interplay between N-WASP and CK2 optimizes clathrin-mediated endocytosis of EGFR.
M. Galovic, D. Xu, L. B. Areces, R. van der Kammen, and M. Innocenti (2011)
J. Cell Sci. 124, 2001-2012
   Abstract »    Full Text »    PDF »
Kinetic Studies of the Arf Activator Arno on Model Membranes in the Presence of Arf Effectors Suggest Control by a Positive Feedback Loop.
D. Stalder, H. Barelli, R. Gautier, E. Macia, C. L. Jackson, and B. Antonny (2011)
J. Biol. Chem. 286, 3873-3883
   Abstract »    Full Text »    PDF »
The MARCKS Protein Plays a Critical Role in Phosphatidylinositol 4,5-Bisphosphate Metabolism and Directed Cell Movement in Vascular Endothelial Cells.
H. Kalwa and T. Michel (2011)
J. Biol. Chem. 286, 2320-2330
   Abstract »    Full Text »    PDF »
Mechanisms of plasma membrane targeting of formin mDia2 through its amino terminal domains.
R. Gorelik, C. Yang, V. Kameswaran, R. Dominguez, and T. Svitkina (2011)
Mol. Biol. Cell 22, 189-201
   Abstract »    Full Text »    PDF »
Estrogen Receptor-{alpha} Promotes Breast Cancer Cell Motility and Invasion via Focal Adhesion Kinase and N-WASP.
A. M. Sanchez, M. I. Flamini, C. Baldacci, L. Goglia, A. R. Genazzani, and T. Simoncini (2010)
Mol. Endocrinol. 24, 2114-2125
   Abstract »    Full Text »    PDF »
Cdc42-Interacting Protein 4 Promotes Breast Cancer Cell Invasion and Formation of Invadopodia through Activation of N-WASp.
C. S. Pichot, C. Arvanitis, S. M. Hartig, S. A. Jensen, J. Bechill, S. Marzouk, J. Yu, J. A. Frost, and S. J. Corey (2010)
Cancer Res. 70, 8347-8356
   Abstract »    Full Text »    PDF »
Plasticity-related Gene 5 (PRG5) Induces Filopodia and Neurite Growth and Impedes Lysophosphatidic Acid- and Nogo-A-mediated Axonal Retraction.
T. Broggini, R. Nitsch, and N. E. Savaskan (2010)
Mol. Biol. Cell 21, 521-537
   Abstract »    Full Text »    PDF »
Regulation of the Actin Cytoskeleton-Plasma Membrane Interplay by Phosphoinositides.
J. Saarikangas, H. Zhao, and P. Lappalainen (2010)
Physiol Rev 90, 259-289
   Abstract »    Full Text »    PDF »
Membrane Organization and Dynamics in Cell Polarity.
K. Orlando and W. Guo (2009)
Cold Spring Harb Perspect Biol 1, a001321
   Abstract »    Full Text »    PDF »
Phosphorylation of WASp is a key regulator of activity and stability in vivo.
M. P. Blundell, G. Bouma, J. Metelo, A. Worth, Y. Calle, L. A. Cowell, L. S. Westerberg, D. A. Moulding, S. Mirando, C. Kinnon, et al. (2009)
PNAS 106, 15738-15743
   Abstract »    Full Text »    PDF »
An allosteric kinase inhibitor binds the p21-activated kinase autoregulatory domain covalently.
J. Viaud and J. R. Peterson (2009)
Mol. Cancer Ther. 8, 2559-2565
   Abstract »    Full Text »    PDF »
The Mechanism of CSF-1-induced Wiskott-Aldrich Syndrome Protein Activation in Vivo: A ROLE FOR PHOSPHATIDYLINOSITOL 3-KINASE AND Cdc42.
M. Cammer, J.-C. Gevrey, M. Lorenz, A. Dovas, J. Condeelis, and D. Cox (2009)
J. Biol. Chem. 284, 23302-23311
   Abstract »    Full Text »    PDF »
Leukocyte trafficking in primary immunodeficiencies.
L. D. Notarangelo and R. Badolato (2009)
J. Leukoc. Biol. 85, 335-343
   Abstract »    Full Text »    PDF »
Construction and Enhancement of a Minimal Genetic AND Logic Gate.
D. J. Sayut, Y. Niu, and L. Sun (2009)
Appl. Envir. Microbiol. 75, 637-642
   Abstract »    Full Text »    PDF »
Grab Your Partner with Both Hands: Cytoskeletal Remodeling by Arp2/3 Signaling.
S. H. Soderling (2009)
Science Signaling 2, pe5
   Abstract »    Full Text »    PDF »
Phosphatidylinositol (4,5)-bisphosphate Modulates Nox5 Localization via an N-Terminal Polybasic Region.
T. Kawahara and J. D. Lambeth (2008)
Mol. Biol. Cell 19, 4020-4031
   Abstract »    Full Text »    PDF »
Regulation of Gic2 Localization and Function by Phosphatidylinositol 4,5-Bisphosphate during the Establishment of Cell Polarity in Budding Yeast.
K. Orlando, J. Zhang, X. Zhang, P. Yue, T. Chiang, E. Bi, and W. Guo (2008)
J. Biol. Chem. 283, 14205-14212
   Abstract »    Full Text »    PDF »
Regulation of Actin Assembly Associated With Protrusion and Adhesion in Cell Migration.
C. Le Clainche and M.-F. Carlier (2008)
Physiol Rev 88, 489-513
   Abstract »    Full Text »    PDF »
Membrane targeting of WAVE2 is not sufficient for WAVE2-dependent actin polymerization: a role for IRSp53 in mediating the interaction between Rac and WAVE2.
W. Abou-Kheir, B. Isaac, H. Yamaguchi, and D. Cox (2008)
J. Cell Sci. 121, 379-390
   Abstract »    Full Text »    PDF »
Membrane association and functional regulation of Sec3 by phospholipids and Cdc42.
X. Zhang, K. Orlando, B. He, F. Xi, J. Zhang, A. Zajac, and W. Guo (2008)
J. Cell Biol. 180, 145-158
   Abstract »    Full Text »    PDF »
Identification of Novel Membrane-binding Domains in Multiple Yeast Cdc42 Effectors.
S. Takahashi and P. M. Pryciak (2007)
Mol. Biol. Cell 18, 4945-4956
   Abstract »    Full Text »    PDF »
Wiskott-Aldrich Syndrome Protein Is a Key Regulator of the Phagocytic Cup Formation in Macrophages.
S. Tsuboi and J. Meerloo (2007)
J. Biol. Chem. 282, 34194-34203
   Abstract »    Full Text »    PDF »
The type III effector EspF coordinates membrane trafficking by the spatiotemporal activation of two eukaryotic signaling pathways.
N. M. Alto, A. W. Weflen, M. J. Rardin, D. Yarar, C. S. Lazar, R. Tonikian, A. Koller, S. S. Taylor, C. Boone, S. S. Sidhu, et al. (2007)
J. Cell Biol. 178, 1265-1278
   Abstract »    Full Text »    PDF »
G{alpha}i generates multiple Pins activation states to link cortical polarity and spindle orientation in Drosophila neuroblasts.
R. W. Nipper, K. H. Siller, N. R. Smith, C. Q. Doe, and K. E. Prehoda (2007)
PNAS 104, 14306-14311
   Abstract »    Full Text »    PDF »
Unregulated actin polymerization by WASp causes defects of mitosis and cytokinesis in X-linked neutropenia.
D. A. Moulding, M. P. Blundell, D. G. Spiller, M. R.H. White, G. O. Cory, Y. Calle, H. Kempski, J. Sinclair, P. J. Ancliff, C. Kinnon, et al. (2007)
J. Exp. Med. 204, 2213-2224
   Abstract »    Full Text »    PDF »
Phosphatidylinositol-4,5 Bisphosphate Produced by PIP5KI{gamma} Regulates Gelsolin, Actin Assembly, and Adhesion Strength of N-Cadherin Junctions.
T. Y. El Sayegh, P. D. Arora, K. Ling, C. Laschinger, P. A. Janmey, R. A. Anderson, and C. A. McCulloch (2007)
Mol. Biol. Cell 18, 3026-3038
   Abstract »    Full Text »    PDF »
Multiple WASP-interacting Protein Recognition Motifs Are Required for a Functional Interaction with N-WASP.
F. C. Peterson, Q. Deng, M. Zettl, K. E. Prehoda, W. A. Lim, M. Way, and B. F. Volkman (2007)
J. Biol. Chem. 282, 8446-8453
   Abstract »    Full Text »    PDF »
Requirement for a Complex of Wiskott-Aldrich Syndrome Protein (WASP) with WASP Interacting Protein in Podosome Formation in Macrophages.
S. Tsuboi (2007)
J. Immunol. 178, 2987-2995
   Abstract »    Full Text »    PDF »
Designing biological systems.
D. A. Drubin, J. C. Way, and P. A. Silver (2007)
Genes & Dev. 21, 242-254
   Abstract »    Full Text »    PDF »
Interaction of the Wiskott-Aldrich syndrome protein with sorting nexin 9 is required for CD28 endocytosis and cosignaling in T cells.
K. Badour, M. K. H. McGavin, J. Zhang, S. Freeman, C. Vieira, D. Filipp, M. Julius, G. B. Mills, and K. A. Siminovitch (2007)
PNAS 104, 1593-1598
   Abstract »    Full Text »    PDF »
c-Abl Interacts with the WAVE2 Signaling Complex to Induce Membrane Ruffling and Cell Spreading.
J. R. Stuart, F. H. Gonzalez, H. Kawai, and Z.-M. Yuan (2006)
J. Biol. Chem. 281, 31290-31297
   Abstract »    Full Text »    PDF »
Chlamydial TARP is a bacterial nucleator of actin.
T. J. Jewett, E. R. Fischer, D. J. Mead, and T. Hackstadt (2006)
PNAS 103, 15599-15604
   Abstract »    Full Text »    PDF »
Tuba stimulates intracellular N-WASP-dependent actin assembly.
E. M. Kovacs, R. S. Makar, and F. B. Gertler (2006)
J. Cell Sci. 119, 2715-2726
   Abstract »    Full Text »    PDF »
Interaction of N-WASP with hnRNPK and Its Role in Filopodia Formation and Cell Spreading.
Y. Yoo, X. Wu, C. Egile, R. Li, and J.-L. Guan (2006)
J. Biol. Chem. 281, 15352-15360
   Abstract »    Full Text »    PDF »
A Complex of Wiskott-Aldrich Syndrome Protein with Mammalian Verprolins Plays an Important Role in Monocyte Chemotaxis..
S. Tsuboi (2006)
J. Immunol. 176, 6576-6585
   Abstract »    Full Text »    PDF »
Protein-tyrosine Kinase and GTPase Signals Cooperate to Phosphorylate and Activate Wiskott-Aldrich Syndrome Protein (WASP)/Neuronal WASP.
E. Torres and M. K. Rosen (2006)
J. Biol. Chem. 281, 3513-3520
   Abstract »    Full Text »    PDF »
Phosphorylation of WASP by the Cdc42-associated Kinase ACK1: DUAL HYDROXYAMINO ACID SPECIFICITY IN A TYROSINE KINASE.
N. Yokoyama, J. Lougheed, and W. T. Miller (2005)
J. Biol. Chem. 280, 42219-42226
   Abstract »    Full Text »    PDF »
Role of the WASP family proteins for Mycobacterium marinum actin tail formation.
L. M. Stamm, M. A. Pak, J. H. Morisaki, S. B. Snapper, K. Rottner, S. Lommel, and E. J. Brown (2005)
PNAS 102, 14837-14842
   Abstract »    Full Text »    PDF »
Lipid rafts and regulation of the cytoskeleton during T cell activation.
K. F. Meiri (2005)
Phil Trans R Soc B 360, 1663-1672
   Abstract »    Full Text »    PDF »
N-WASP deficiency impairs EGF internalization and actin assembly at clathrin-coated pits.
S. Benesch, S. Polo, F. P. L. Lai, K. I. Anderson, T. E. B. Stradal, J. Wehland, and K. Rottner (2005)
J. Cell Sci. 118, 3103-3115
   Abstract »    Full Text »    PDF »
A Dictyostelium Homologue of WASP Is Required for Polarized F-Actin Assembly during Chemotaxis.
S. A. Myers, J. W. Han, Y. Lee, R. A. Firtel, and C. Y. Chung (2005)
Mol. Biol. Cell 16, 2191-2206
   Abstract »    Full Text »    PDF »
Two Distinct Head-Tail Interfaces Cooperate to Suppress Activation of Vinculin by Talin.
D. M. Cohen, H. Chen, R. P. Johnson, B. Choudhury, and S. W. Craig (2005)
J. Biol. Chem. 280, 17109-17117
   Abstract »    Full Text »    PDF »
The nucleotide switch in Cdc42 modulates coupling between the GTPase-binding and allosteric equilibria of Wiskott-Aldrich syndrome protein.
D. W. Leung and M. K. Rosen (2005)
PNAS 102, 5685-5690
   Abstract »    Full Text »    PDF »
Phosphatidylinositol-4,5-bisphosphate hydrolysis directs actin remodeling during phagocytosis.
C. C. Scott, W. Dobson, R. J. Botelho, N. Coady-Osberg, P. Chavrier, D. A. Knecht, C. Heath, P. Stahl, and S. Grinstein (2005)
J. Cell Biol. 169, 139-149
   Abstract »    Full Text »    PDF »
The NF2 Tumor Suppressor Merlin and the ERM Proteins Interact with N-WASP and Regulate Its Actin Polymerization Function.
N. Manchanda, A. Lyubimova, H.-Y. H. Ho, M. F. James, J. F. Gusella, N. Ramesh, S. B. Snapper, and V. Ramesh (2005)
J. Biol. Chem. 280, 12517-12522
   Abstract »    Full Text »    PDF »
Spatially Distinct Binding of Cdc42 to PAK1 and N-WASP in Breast Carcinoma Cells.
M. Parsons, J. Monypenny, S. M. Ameer-Beg, T. H. Millard, L. M. Machesky, M. Peter, M. D. Keppler, G. Schiavo, R. Watson, J. Chernoff, et al. (2005)
Mol. Cell. Biol. 25, 1680-1695
   Abstract »    Full Text »    PDF »
Concentric zones of active RhoA and Cdc42 around single cell wounds.
H. A. Benink and W. M. Bement (2005)
J. Cell Biol. 168, 429-439
   Abstract »    Full Text »    PDF »
Abelson-interactor-1 promotes WAVE2 membrane translocation and Abelson-mediated tyrosine phosphorylation required for WAVE2 activation.
Y. Leng, J. Zhang, K. Badour, E. Arpaia, S. Freeman, P. Cheung, M. Siu, and K. Siminovitch (2005)
PNAS 102, 1098-1103
   Abstract »    Full Text »    PDF »
Cortactin regulates cell migration through activation of N-WASP.
J. R. Kowalski, C. Egile, S. Gil, S. B. Snapper, R. Li, and S. M. Thomas (2005)
J. Cell Sci. 118, 79-87
   Abstract »    Full Text »    PDF »
Mechanisms of WASp-mediated hematologic and immunologic disease.
S. Burns, G. O. Cory, W. Vainchenker, and A. J. Thrasher (2004)
Blood 104, 3454-3462
   Abstract »    Full Text »    PDF »
Apical localization of actin patches and vacuolar dynamics in Ashbya gossypii depend on the WASP homolog Wal1p.
A. Walther and J. Wendland (2004)
J. Cell Sci. 117, 4947-4958
   Abstract »    Full Text »    PDF »
Regulation of WASP/WAVE proteins: making a long story short.
G. Bompard and E. Caron (2004)
J. Cell Biol. 166, 957-962
   Abstract »    Full Text »    PDF »
The Activity of the Plexin-A1 Receptor Is Regulated by Rac.
L. J. Turner, S. Nicholls, and A. Hall (2004)
J. Biol. Chem. 279, 33199-33205
   Abstract »    Full Text »    PDF »
Phosphatidylinositol 4,5-Bisphosphate Regulates Adipocyte Actin Dynamics and GLUT4 Vesicle Recycling.
M. Kanzaki, M. Furukawa, W. Raab, and J. E. Pessin (2004)
J. Biol. Chem. 279, 30622-30633
   Abstract »    Full Text »    PDF »
Regulated Membrane Trafficking of the Insulin-Responsive Glucose Transporter 4 in Adipocytes.
R. T. Watson, M. Kanzaki, and J. E. Pessin (2004)
Endocr. Rev. 25, 177-204
   Abstract »    Full Text »    PDF »
Focal Adhesion Kinase Regulation of N-WASP Subcellular Localization and Function.
X. Wu, S. Suetsugu, L. A. Cooper, T. Takenawa, and J.-L. Guan (2004)
J. Biol. Chem. 279, 9565-9576
   Abstract »    Full Text »    PDF »
Activation of Type I Phosphatidylinositol 4-Phosphate 5-Kinase Isoforms by the Rho GTPases, RhoA, Rac1, and Cdc42.
P. A. O. Weernink, K. Meletiadis, S. Hommeltenberg, M. Hinz, H. Ishihara, M. Schmidt, and K. H. Jakobs (2004)
J. Biol. Chem. 279, 7840-7849
   Abstract »    Full Text »    PDF »
WASp is required for the correct temporal morphogenesis of rhabdomere microvilli.
A. C. Zelhof and R. W. Hardy (2004)
J. Cell Biol. 164, 417-426
   Abstract »    Full Text »    PDF »
Regulated Exocytosis in Neuroendocrine Cells: A Role for Subplasmalemmal Cdc42/N-WASP-induced Actin Filaments.
S. Gasman, S. Chasserot-Golaz, M. Malacombe, M. Way, and M.-F. Bader (2004)
Mol. Biol. Cell 15, 520-531
   Abstract »    Full Text »    PDF »
Visualization of spatially and temporally regulated N-WASP activity during cytoskeletal reorganization in living cells.
M. E. Ward, J. Y. Wu, and Y. Rao (2004)
PNAS 101, 970-974
   Abstract »    Full Text »    PDF »
Fyn and PTP-PEST-mediated Regulation of Wiskott-Aldrich Syndrome Protein (WASp) Tyrosine Phosphorylation Is Required for Coupling T Cell Antigen Receptor Engagement to WASp Effector Function and T Cell Activation.
K. Badour, J. Zhang, F. Shi, Y. Leng, M. Collins, and K. A. Siminovitch (2004)
J. Exp. Med. 199, 99-112
   Abstract »    Full Text »    PDF »
Translocation of N-WASP by Nuclear Localization and Export Signals into the Nucleus Modulates Expression of HSP90.
S. Suetsugu and T. Takenawa (2003)
J. Biol. Chem. 278, 42515-42523
   Abstract »    Full Text »    PDF »
Reprogramming Control of an Allosteric Signaling Switch Through Modular Recombination.
J. E. Dueber, B. J. Yeh, K. Chak, and W. A. Lim (2003)
Science 301, 1904-1908
   Abstract »    Full Text »    PDF »
Regulation of Actin Dynamics by WASP Family Proteins.
H. Miki and T. Takenawa (2003)
J. Biochem. 134, 309-313
   Abstract »    Full Text »    PDF »
Gene therapy for Wiskott-Aldrich syndrome: rescue of T-cell signaling and amelioration of colitis upon transplantation of retrovirally transduced hematopoietic stem cells in mice.
C. Klein, D. Nguyen, C.-H. Liu, A. Mizoguchi, A. K. Bhan, H. Miki, T. Takenawa, F. S. Rosen, F. W. Alt, R. C. Mulligan, et al. (2003)
Blood 101, 2159-2166
   Abstract »    Full Text »    PDF »
Mouse MIM, a Tissue-specific Regulator of Cytoskeletal Dynamics, Interacts with ATP-Actin Monomers through Its C-terminal WH2 Domain.
P. K. Mattila, M. Salminen, T. Yamashiro, and P. Lappalainen (2003)
J. Biol. Chem. 278, 8452-8459
   Abstract »    Full Text »    PDF »
Neural Wiskott-Aldrich Syndrome Protein Is Recruited to Rafts and Associates with Endophilin A in Response to Epidermal Growth Factor.
M. Otsuki, T. Itoh, and T. Takenawa (2003)
J. Biol. Chem. 278, 6461-6469
   Abstract »    Full Text »    PDF »
Phosphorylation of Tyrosine 291 Enhances the Ability of WASp to Stimulate Actin Polymerization and Filopodium Formation.
G. O. C. Cory, R. Garg, R. Cramer, and A. J. Ridley (2002)
J. Biol. Chem. 277, 45115-45121
   Abstract »    Full Text »    PDF »
Inhibition of Phosphatidylinositol-4-phosphate 5-Kinase Ialpha Impairs Localized Actin Remodeling and Suppresses Phagocytosis.
M. G. Coppolino, R. Dierckman, J. Loijens, R. F. Collins, M. Pouladi, J. Jongstra-Bilen, A. D. Schreiber, W. S. Trimble, R. Anderson, and S. Grinstein (2002)
J. Biol. Chem. 277, 43849-43857
   Abstract »    Full Text »    PDF »
Motility Determinants in WASP Family Proteins.
D. Yarar, J. A. D'Alessio, R. L. Jeng, and M. D. Welch (2002)
Mol. Biol. Cell 13, 4045-4059
   Abstract »    Full Text »    PDF »
Pairs of dipeptides synergistically activate the binding of substrate by ubiquitin ligase through dissociation of its autoinhibitory domain.
F. Du, F. Navarro-Garcia, Z. Xia, T. Tasaki, and A. Varshavsky (2002)
PNAS 99, 14110-14115
   Abstract »    Full Text »    PDF »
Phosphatidylinositol 4,5-Biphosphate (PIP2)-induced Vesicle Movement Depends on N-WASP and Involves Nck, WIP, and Grb2.
S. Benesch, S. Lommel, A. Steffen, T. E. B. Stradal, N. Scaplehorn, M. Way, J. Wehland, and K. Rottner (2002)
J. Biol. Chem. 277, 37771-37776
   Abstract »    Full Text »    PDF »
Send Me a Message: The FASEB Conference on Signal Transduction Salt Lake City, UT June 29-July 3, 2002.
W. A. Wells (2002)
J. Cell Biol. 158, 994-995
   Full Text »    PDF »
Lateral Sequestration of Phosphatidylinositol 4,5-Bisphosphate by the Basic Effector Domain of Myristoylated Alanine-rich C Kinase Substrate Is Due to Nonspecific Electrostatic Interactions.
J. Wang, A. Gambhir, G. Hangyas-Mihalyne, D. Murray, U. Golebiewska, and S. McLaughlin (2002)
J. Biol. Chem. 277, 34401-34412
   Abstract »    Full Text »    PDF »
Remodeling of organelle-bound actin is required for yeast vacuole fusion.
G. Eitzen, L. Wang, N. Thorngren, and W. Wickner (2002)
J. Cell Biol. 158, 669-679
   Abstract »    Full Text »    PDF »
Normal Arp2/3 complex activation in platelets lacking WASp.
H. Falet, K. M. Hoffmeister, R. Neujahr, and J. H. Hartwig (2002)
Blood 100, 2113-2122
   Abstract »    Full Text »    PDF »
Identification of Novel SH3 Domain Ligands for the Src Family Kinase Hck. WISKOTT-ALDRICH SYNDROME PROTEIN (WASP), WASP-INTERACTING PROTEIN (WIP), AND ELMO1.
M. P. Scott, F. Zappacosta, E. Y. Kim, R. S. Annan, and W. T. Miller (2002)
J. Biol. Chem. 277, 28238-28246
   Abstract »    Full Text »    PDF »
The Cdc42 and Rac1 GTPases are required for capillary lumen formation in three-dimensional extracellular matrices.
K. J. Bayless and G. E. Davis (2002)
J. Cell Sci. 115, 1123-1136
   Abstract »    Full Text »    PDF »
Regulation of Protein Transport from the Golgi Complex to the Endoplasmic Reticulum by CDC42 and N-WASP.
A. Luna, O. B. Matas, J. A. Martinez-Menarguez, E. Mato, J. M. Duran, J. Ballesta, M. Way, and G. Egea (2002)
Mol. Biol. Cell 13, 866-879
   Abstract »    Full Text »    PDF »

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882