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Science 291 (5504): 664-667

Copyright © 2001 by the American Association for the Advancement of Science

Glycolipid Antigen Processing for Presentation by CD1d Molecules

Theodore I. Prigozy,1 Olga Naidenko,1 Pankaj Qasba,2 Dirk Elewaut,1 Laurent Brossay,1* Archana Khurana,1 Takenori Natori,3 Yasuhiko Koezuka,3 Ashok Kulkarni,4 Mitchell Kronenberg1dagger

The requirement for processing glycolipid antigens in T cell recognition was examined with mouse CD1d-mediated responses to glycosphingolipids (GSLs). Although some disaccharide GSL antigens can be recognized without processing, the responses to three other antigens, including the disaccharide GSL Gal(alpha 1rightarrow 2)GalCer (Gal, galactose; GalCer, galactosylceramide), required removal of the terminal sugars to permit interaction with the T cell receptor. A lysosomal enzyme, alpha -galactosidase A, was responsible for the processing of Gal(alpha 1rightarrow 2)GalCer to generate the antigenic monosaccharide epitope. These data demonstrate a carbohydrate antigen processing system analogous to that used for peptides and an ability of T cells to recognize processed fragments of complex glycolipids.

1 Division of Developmental Immunology, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA.
2 Developmental and Metabolic Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.
3 Pharmaceutical Research Laboratory, Kirin Brewery, Gunma 370-12, Japan.
4 Functional Genomics Unit, National Institute of Dental and Craniofacial Research, Bethesda, MD 20892, USA.
*   Present address: Department of Molecular Microbiology and Immunology, Brown University, Providence, RI 02912, USA.

dagger    To whom correspondence should be addressed. E-mail: mitch{at}liai.org


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