Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Logo for

Science 291 (5512): 2408-2411

Copyright © 2001 by the American Association for the Advancement of Science

Receptor-Mediated Activation of Heterotrimeric G-Proteins in Living Cells

Chris Janetopoulos,* Tian Jin,* Peter Devreotes*dagger

Receptor-mediated activation of heterotrimeric GTP-binding proteins (G-proteins) was visualized in living Dictyostelium discoideum cells by monitoring fluorescence resonance energy transfer (FRET) between alpha - and beta - subunits fused to cyan and yellow fluorescent proteins. The G-protein heterotrimer rapidly dissociated and reassociated upon addition and removal of chemoattractant. During continuous stimulation, G-protein activation reached a dose-dependent steady-state level. Even though physiological responses subsided, the activation did not decline. Thus, adaptation occurs at another point in the signaling pathway, and occupied receptors, whether or not they are phosphorylated, catalyze the G-protein cycle. Construction of similar energy-transfer pairs of mammalian G-proteins should enable direct in situ mechanistic studies and applications such as drug screening and identifying ligands of newly found G-protein-coupled receptors.

Department of Biological Chemistry, Johns Hopkins Medical Institutions, Baltimore, MD 21205, USA.
*   Present address: Department of Cell Biology and Anatomy, Johns Hopkins Medical Institutions, Baltimore, MD 21205, USA.

dagger    To whom correspondence should be addressed.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Do Plants Contain G Protein-Coupled Receptors?.
B. Taddese, G. J. G. Upton, G. R. Bailey, S. R. D. Jordan, N. Y. Abdulla, P. J. Reeves, and C. A. Reynolds (2014)
Plant Physiology 164, 287-307
   Abstract »    Full Text »    PDF »
Phosphorylation of chemoattractant receptors regulates chemotaxis, actin reorganization and signal relay.
J. A. Brzostowski, S. Sawai, O. Rozov, X.-h. Liao, D. Imoto, C. A. Parent, and A. R. Kimmel (2013)
J. Cell Sci. 126, 4614-4626
   Abstract »    Full Text »    PDF »
Real-time endocytosis imaging as a rapid assay of ligand-GPCR binding in single cells.
L.-H. Zheng, C.-H. Wang, S.-J. Shang, X.-Y. Zhang, Y.-S. Wang, Q.-H. Wu, M.-Q. Hu, Z.-Y. Chai, X. Wu, H. Zheng, et al. (2013)
Am J Physiol Cell Physiol 305, C751-C760
   Abstract »    Full Text »    PDF »
Chemoattractant stimulation of TORC2 is regulated by receptor/G protein-targeted inhibitory mechanisms that function upstream and independently of an essential GEF/Ras activation pathway in Dictyostelium.
X.-H. Liao, J. Buggey, Y. K. Lee, and A. R. Kimmel (2013)
Mol. Biol. Cell 24, 2146-2155
   Abstract »    Full Text »    PDF »
Heterotrimeric G protein signalling in the plant kingdom.
D. Urano, J.-G. Chen, J. R. Botella, and A. M. Jones (2013)
Open Bio 3, 120186
   Abstract »    Full Text »    PDF »
"Round Up the Usual Suspects": A Comment on Nonexistent Plant G Protein-Coupled Receptors.
D. Urano and A. M. Jones (2013)
Plant Physiology 161, 1097-1102
   Full Text »    PDF »
Delineating the core regulatory elements crucial for directed cell migration by examining folic-acid-mediated responses.
K. Srinivasan, G. A. Wright, N. Hames, M. Housman, A. Roberts, K. J. Aufderheide, and C. Janetopoulos (2013)
J. Cell Sci. 126, 221-233
   Abstract »    Full Text »    PDF »
Signal Activation and Inactivation by the G{alpha} Helical Domain: A Long-Neglected Partner in G Protein Signaling.
H. G. Dohlman and J. C. Jones (2012)
Science Signaling 5, re2
   Abstract »    Full Text »    PDF »
Fluorescence/Bioluminescence Resonance Energy Transfer Techniques to Study G-Protein-Coupled Receptor Activation and Signaling.
M. J. Lohse, S. Nuber, and C. Hoffmann (2012)
Pharmacol. Rev. 64, 299-336
   Abstract »    Full Text »    PDF »
Chemoattractant Signaling in Dictyostelium: Adaptation and Amplification.
P. A. Iglesias (2012)
Science Signaling 5, pe8
   Abstract »    Full Text »    PDF »
Diverse Sensitivity Thresholds in Dynamic Signaling Responses by Social Amoebae.
C. J. Wang, A. Bergmann, B. Lin, K. Kim, and A. Levchenko (2012)
Science Signaling 5, ra17
   Abstract »    Full Text »    PDF »
Incoherent Feedforward Control Governs Adaptation of Activated Ras in a Eukaryotic Chemotaxis Pathway.
K. Takeda, D. Shao, M. Adler, P. G. Charest, W. F. Loomis, H. Levine, A. Groisman, W.-J. Rappel, and R. A. Firtel (2012)
Science Signaling 5, ra2
   Abstract »    Full Text »    PDF »
Tyrosine Phosphorylation of the G{alpha}-Interacting Protein GIV Promotes Activation of Phosphoinositide 3-Kinase During Cell Migration.
C. Lin, J. Ear, Y. Pavlova, Y. Mittal, I. Kufareva, M. Ghassemian, R. Abagyan, M. Garcia-Marcos, and P. Ghosh (2011)
Science Signaling 4, ra64
   Abstract »    Full Text »    PDF »
Two Distinct Aspects of Coupling between G{alpha}i Protein and G Protein-activated K+ Channel (GIRK) Revealed by Fluorescently Labeled G{alpha}i3 Protein Subunits.
S. Berlin, V. A. Tsemakhovich, R. Castel, T. Ivanina, C. W. Dessauer, T. Keren-Raifman, and N. Dascal (2011)
J. Biol. Chem. 286, 33223-33235
   Abstract »    Full Text »    PDF »
A Novel KCNJ2 Nonsense Mutation, S369X, Impedes Trafficking and Causes a Limited Form of Andersen-Tawil Syndrome.
T. Doi, T. Makiyama, T. Morimoto, Y. Haruna, K. Tsuji, S. Ohno, M. Akao, Y. Takahashi, T. Kimura, and M. Horie (2011)
Circ Cardiovasc Genet 4, 253-260
   Abstract »    Full Text »    PDF »
Differential Signaling of the Endogenous Agonists at the {beta}2-Adrenergic Receptor.
S. Reiner, M. Ambrosio, C. Hoffmann, and M. J. Lohse (2010)
J. Biol. Chem. 285, 36188-36198
   Abstract »    Full Text »    PDF »
Costars, a Dictyostelium protein similar to the C-terminal domain of STARS, regulates the actin cytoskeleton and motility.
T.-L. Pang, F.-C. Chen, Y.-L. Weng, H.-C. Liao, Y.-H. Yi, C.-L. Ho, C.-H. Lin, and M.-Y. Chen (2010)
J. Cell Sci. 123, 3745-3755
   Abstract »    Full Text »    PDF »
Cells navigate with a local-excitation, global-inhibition-biased excitable network.
Y. Xiong, C.-H. Huang, P. A. Iglesias, and P. N. Devreotes (2010)
PNAS 107, 17079-17086
   Abstract »    Full Text »    PDF »
Coupling Mechanism of a GPCR and a Heterotrimeric G Protein During Chemoattractant Gradient Sensing in Dictyostelium.
X. Xu, T. Meckel, J. A. Brzostowski, J. Yan, M. Meier-Schellersheim, and T. Jin (2010)
Science Signaling 3, ra71
   Abstract »    Full Text »    PDF »
Mobility of G proteins is heterogeneous and polarized during chemotaxis.
F. van Hemert, M. D. Lazova, B. E. Snaar-Jagaska, and T. Schmidt (2010)
J. Cell Sci. 123, 2922-2930
   Abstract »    Full Text »    PDF »
Ras-mediated activation of the TORC2-PKB pathway is critical for chemotaxis.
H. Cai, S. Das, Y. Kamimura, Y. Long, C. A. Parent, and P. N. Devreotes (2010)
J. Cell Biol. 190, 233-245
   Abstract »    Full Text »    PDF »
Imaging of persistent cAMP signaling by internalized G protein-coupled receptors.
D. Calebiro, V. O. Nikolaev, and M. J. Lohse (2010)
J. Mol. Endocrinol. 45, 1-8
   Abstract »    Full Text »    PDF »
Heterotrimeric G Protein Signaling Outside the Realm of Seven Transmembrane Domain Receptors.
C. Marty and R. D. Ye (2010)
Mol. Pharmacol. 78, 12-18
   Abstract »    Full Text »    PDF »
G Proteins in Reverse Mode: RECEPTOR-MEDIATED GTP RELEASE INHIBITS G PROTEIN AND EFFECTOR FUNCTION.
L. G. Hommers, C. Klenk, C. Dees, and M. Bunemann (2010)
J. Biol. Chem. 285, 8227-8233
   Abstract »    Full Text »    PDF »
Putting G protein-coupled receptor-mediated activation of phospholipase C in the limelight.
T. Balla (2010)
J. Gen. Physiol. 135, 77-80
   Full Text »    PDF »
The Signaling Mechanisms Underlying Cell Polarity and Chemotaxis.
F. Wang (2009)
Cold Spring Harb Perspect Biol 1, a002980
   Abstract »    Full Text »    PDF »
Dynamic localization of G proteins in Dictyostelium discoideum.
C. A. Elzie, J. Colby, M. A. Sammons, and C. Janetopoulos (2009)
J. Cell Sci. 122, 2597-2603
   Abstract »    Full Text »    PDF »
The Group Migration of Dictyostelium Cells Is Regulated by Extracellular Chemoattractant Degradation.
G. L. Garcia, E. C. Rericha, C. D. Heger, P. K. Goldsmith, and C. A. Parent (2009)
Mol. Biol. Cell 20, 3295-3304
   Abstract »    Full Text »    PDF »
Evidence for a Second, High Affinity G{beta}{gamma} Binding Site on G{alpha}i1(GDP) Subunits.
J. Wang, P. Sengupta, Y. Guo, U. Golebiewska, and S. Scarlata (2009)
J. Biol. Chem. 284, 16906-16913
   Abstract »    Full Text »    PDF »
Modeling of G-protein-coupled Receptor Signaling Pathways.
J. J. Linderman (2009)
J. Biol. Chem. 284, 5427-5431
   Full Text »    PDF »
Activation-dependent Hindrance of Photoreceptor G Protein Diffusion by Lipid Microdomains.
Q. Wang, X. Zhang, L. Zhang, F. He, G. Zhang, M. Jamrich, and T. G. Wensel (2008)
J. Biol. Chem. 283, 30015-30024
   Abstract »    Full Text »    PDF »
Immunostaining evidence for PI(4,5)P2 localization at the leading edge of chemoattractant-stimulated HL-60 cells.
V. P. Sharma, V. DesMarais, C. Sumners, G. Shaw, and A. Narang (2008)
J. Leukoc. Biol. 84, 440-447
   Abstract »    Full Text »    PDF »
Activation of G{alpha}i3 triggers cell migration via regulation of GIV.
P. Ghosh, M. Garcia-Marcos, S. J. Bornheimer, and M. G. Farquhar (2008)
J. Cell Biol. 182, 381-393
   Abstract »    Full Text »    PDF »
Dissociation of Heterotrimeric G Proteins in Cells.
N. A. Lambert (2008)
Science Signaling 1, re5
   Abstract »    Full Text »    PDF »
Characterization of the Arabidopsis Heterotrimeric G Protein.
S. Wang, S. M. Assmann, and N. V. Fedoroff (2008)
J. Biol. Chem. 283, 13913-13922
   Abstract »    Full Text »    PDF »
A spatially restricted increase in receptor mobility is involved in directional sensing during Dictyostelium discoideum chemotaxis.
S. de Keijzer, A. Serge, F. van Hemert, P. H. M. Lommerse, G. E. M. Lamers, H. P. Spaink, T. Schmidt, and B. E. Snaar-Jagalska (2008)
J. Cell Sci. 121, 1750-1757
   Abstract »    Full Text »    PDF »
Caveolin-1 alters Ca2+ signal duration through specific interaction with the G{alpha}q family of G proteins.
P. Sengupta, F. Philip, and S. Scarlata (2008)
J. Cell Sci. 121, 1363-1372
   Abstract »    Full Text »    PDF »
Four key signaling pathways mediating chemotaxis in Dictyostelium discoideum.
D. M. Veltman, I. Keizer-Gunnik, and P. J.M. Van Haastert (2008)
J. Cell Biol. 180, 747-753
   Abstract »    Full Text »    PDF »
Locally controlled inhibitory mechanisms are involved in eukaryotic GPCR-mediated chemosensing.
X. Xu, M. Meier-Schellersheim, J. Yan, and T. Jin (2007)
J. Cell Biol. 178, 141-153
   Abstract »    Full Text »    PDF »
ZBP1 enhances cell polarity and reduces chemotaxis.
K. Lapidus, J. Wyckoff, G. Mouneimne, M. Lorenz, L. Soon, J. S. Condeelis, and R. H. Singer (2007)
J. Cell Sci. 120, 3173-3178
   Abstract »    Full Text »    PDF »
Localization of the Mouse 5-Hydroxytryptamine1A Receptor in Lipid Microdomains Depends on Its Palmitoylation and Is Involved in Receptor-Mediated Signaling.
U. Renner, K. Glebov, T. Lang, E. Papusheva, S. Balakrishnan, B. Keller, D. W. Richter, R. Jahn, and E. Ponimaskin (2007)
Mol. Pharmacol. 72, 502-513
   Abstract »    Full Text »    PDF »
A FRET map of membrane anchors suggests distinct microdomains of heterotrimeric G proteins.
D. Abankwa and H. Vogel (2007)
J. Cell Sci. 120, 2953-2962
   Abstract »    Full Text »    PDF »
Attenuation of Phospholipid Signaling Provides a Novel Mechanism for the Action of Valproic Acid.
X. Xu, A. Muller-Taubenberger, K. E. Adley, N. Pawolleck, V. W. Y. Lee, C. Wiedemann, T. S. Sihra, M. Maniak, T. Jin, and R. S. B. Williams (2007)
Eukaryot. Cell 6, 899-906
   Abstract »    Full Text »    PDF »
Real-Time Analysis of Agonist-Induced Activation of Protease-Activated Receptor 1/G{alpha}i1 Protein Complex Measured by Bioluminescence Resonance Energy Transfer in Living Cells.
M. A. Ayoub, D. Maurel, V. Binet, M. Fink, L. Prezeau, H. Ansanay, and J.-P. Pin (2007)
Mol. Pharmacol. 71, 1329-1340
   Abstract »    Full Text »    PDF »
Evidence for association of GABAB receptors with Kir3 channels and regulators of G protein signalling (RGS4) proteins.
C. E. Fowler, P. Aryal, K. F. Suen, and P. A. Slesinger (2007)
J. Physiol. 580, 51-65
   Abstract »    Full Text »    PDF »
G Protein-coupled Receptor Kinase and beta-Arrestin-mediated Desensitization of the Angiotensin II Type 1A Receptor Elucidated by Diacylglycerol Dynamics.
J. D. Violin, S. M. DeWire, W. G. Barnes, and R. J. Lefkowitz (2006)
J. Biol. Chem. 281, 36411-36419
   Abstract »    Full Text »    PDF »
Some G protein heterotrimers physically dissociate in living cells.
G. J. Digby, R. M. Lober, P. R. Sethi, and N. A. Lambert (2006)
PNAS 103, 17789-17794
   Abstract »    Full Text »    PDF »
GS Activation Is Time-limiting in Initiating Receptor-mediated Signaling.
P. Hein, F. Rochais, C. Hoffmann, S. Dorsch, V. O. Nikolaev, S. Engelhardt, C. H. Berlot, M. J. Lohse, and M. Bunemann (2006)
J. Biol. Chem. 281, 33345-33351
   Abstract »    Full Text »    PDF »
Systems analysis of PKA-mediated phosphorylation gradients in live cardiac myocytes.
J. J. Saucerman, J. Zhang, J. C. Martin, L. X. Peng, A. E. Stenbit, R. Y. Tsien, and A. D. McCulloch (2006)
PNAS 103, 12923-12928
   Abstract »    Full Text »    PDF »
Stable Association between G{alpha}q and Phospholipase Cbeta1 in Living Cells.
L. Dowal, P. Provitera, and S. Scarlata (2006)
J. Biol. Chem. 281, 23999-24014
   Abstract »    Full Text »    PDF »
Directional sensing in eukaryotic chemotaxis: A balanced inactivation model.
H. Levine, D. A. Kessler, and W.-J. Rappel (2006)
PNAS 103, 9761-9766
   Abstract »    Full Text »    PDF »
Single-molecule analysis of chemoattractant-stimulated membrane recruitment of a PH-domain-containing protein.
S. Matsuoka, M. Iijima, T. M. Watanabe, H. Kuwayama, T. Yanagida, P. N. Devreotes, and M. Ueda (2006)
J. Cell Sci. 119, 1071-1079
   Abstract »    Full Text »    PDF »
Gbeta{gamma} Inhibits G{alpha} GTPase-activating Proteins by Inhibition of G{alpha}-GTP Binding during Stimulation by Receptor.
W. Tang, Y. Tu, S. K. Nayak, J. Woodson, M. Jehl, and E. M. Ross (2006)
J. Biol. Chem. 281, 4746-4753
   Abstract »    Full Text »    PDF »
Gi{alpha} and G{beta} subunits both define selectivity of G protein activation by {alpha}2-adrenergic receptors.
S. K. Gibson and A. G. Gilman (2006)
PNAS 103, 212-217
   Abstract »    Full Text »    PDF »
Imaging Signal Transduction in Living Cells with Fluorescent Proteins.
M. R. Philips (2005)
Sci. STKE 2005, tr28
   Abstract »    Full Text »    PDF »
Dynamics of receptor/G protein coupling in living cells.
P. Hein, M. Frank, C. Hoffmann, M. J. Lohse, and M. Bunemann (2005)
EMBO J. 24, 4106-4114
   Abstract »    Full Text »    PDF »
The Phosducin-Like Protein PhLP1 Is Essential for G{beta}{gamma} Dimer Formation in Dictyostelium discoideum.
J. C. Knol, R. Engel, M. Blaauw, A. J. W. G. Visser, and P. J. M. van Haastert (2005)
Mol. Cell. Biol. 25, 8393-8400
   Abstract »    Full Text »    PDF »
Abnormal KCNQ1 trafficking influences disease pathogenesis in hereditary long QT syndromes (LQT1).
A. J. Wilson, K. V. Quinn, F. M. Graves, M. Bitner-Glindzicz, and A. Tinker (2005)
Cardiovasc Res 67, 476-486
   Abstract »    Full Text »    PDF »
G Protein Activation without Subunit Dissociation Depends on a G{alpha}i-specific Region.
M. Frank, L. Thumer, M. J. Lohse, and M. Bunemann (2005)
J. Biol. Chem. 280, 24584-24590
   Abstract »    Full Text »    PDF »
Mining the Receptorome.
B. N. Armbruster and B. L. Roth (2005)
J. Biol. Chem. 280, 5129-5132
   Full Text »    PDF »
Quantitative Imaging of Single Live Cells Reveals Spatiotemporal Dynamics of Multistep Signaling Events of Chemoattractant Gradient Sensing in Dictyostelium.
X. Xu, M. Meier-Schellersheim, X. Jiao, L. E. Nelson, and T. Jin (2005)
Mol. Biol. Cell 16, 676-688
   Abstract »    Full Text »    PDF »
Constitutively Active G Protein-coupled Receptor Mutants Block Dictyostelium Development.
M. Zhang, M. Goswami, and D. Hereld (2005)
Mol. Biol. Cell 16, 562-572
   Abstract »    Full Text »    PDF »
Dictyostelium PAKc Is Required for Proper Chemotaxis.
S. Lee, F. Rivero, K. C. Park, E. Huang, S. Funamoto, and R. A. Firtel (2004)
Mol. Biol. Cell 15, 5456-5469
   Abstract »    Full Text »    PDF »
Localized Ras signaling at the leading edge regulates PI3K, cell polarity, and directional cell movement.
A. T. Sasaki, C. Chun, K. Takeda, and R. A. Firtel (2004)
J. Cell Biol. 167, 505-518
   Abstract »    Full Text »    PDF »
Live Cell Imaging of Gs and the {beta}2-Adrenergic Receptor Demonstrates That Both {alpha}s and {beta}1{gamma}7 Internalize upon Stimulation and Exhibit Similar Trafficking Patterns That Differ from That of the {beta}2-Adrenergic Receptor.
T. R. Hynes, S. M. Mervine, E. A. Yost, J. L. Sabo, and C. H. Berlot (2004)
J. Biol. Chem. 279, 44101-44112
   Abstract »    Full Text »    PDF »
Moving Forward: Mechanisms of Chemoattractant Gradient Sensing.
J. Franca-Koh and P. N. Devreotes (2004)
Physiology 19, 300-308
   Abstract »    Full Text »    PDF »
A Fluorescence Resonance Energy Transfer-based Sensor Indicates that Receptor Access to a G Protein Is Unrestricted in a Living Mammalian Cell.
I. Azpiazu and N. Gautam (2004)
J. Biol. Chem. 279, 27709-27718
   Abstract »    Full Text »    PDF »
Chemoattractant-induced phosphatidylinositol 3,4,5-trisphosphate accumulation is spatially amplified and adapts, independent of the actin cytoskeleton.
C. Janetopoulos, L. Ma, P. N. Devreotes, and P. A. Iglesias (2004)
PNAS 101, 8951-8956
   Abstract »    Full Text »    PDF »
Sensitization of Dictyostelium chemotaxis by phosphoinositide-3-kinase-mediated self-organizing signalling patches.
M. Postma, J. Roelofs, J. Goedhart, H. M. Loovers, A. J. W. G. Visser, and P. J. M. Van Haastert (2004)
J. Cell Sci. 117, 2925-2935
   Abstract »    Full Text »    PDF »
On the Mechanism and Significance of Ligand-induced Internalization of Human Neutrophil Chemokine Receptors CXCR1 and CXCR2.
J. J. Rose, J. F. Foley, P. M. Murphy, and S. Venkatesan (2004)
J. Biol. Chem. 279, 24372-24386
   Abstract »    Full Text »    PDF »
A G{alpha}-dependent pathway that antagonizes multiple chemoattractant responses that regulate directional cell movement.
J. A. Brzostowski, C. A. Parent, and A. R. Kimmel (2004)
Genes & Dev. 18, 805-815
   Abstract »    Full Text »    PDF »
Gi protein activation in intact cells involves subunit rearrangement rather than dissociation.
M. Bunemann, M. Frank, and M. J. Lohse (2003)
PNAS 100, 16077-16082
   Abstract »    Full Text »    PDF »
Uniform cAMP Stimulation of Dictyostelium Cells Induces Localized Patches of Signal Transduction and Pseudopodia.
M. Postma, J. Roelofs, J. Goedhart, T. W.J. Gadella, A. J.W.G. Visser, and P. J.M. Van Haastert (2003)
Mol. Biol. Cell 14, 5019-5027
   Abstract »    Full Text »    PDF »
Regulators of G Protein Signaling and Transient Activation of Signaling: EXPERIMENTAL AND COMPUTATIONAL ANALYSIS REVEALS NEGATIVE AND POSITIVE FEEDBACK CONTROLS ON G PROTEIN ACTIVITY.
N. Hao, N. Yildirim, Y. Wang, T. C. Elston, and H. G. Dohlman (2003)
J. Biol. Chem. 278, 46506-46515
   Abstract »    Full Text »    PDF »
Phosducin-like proteins in Dictyostelium discoideum: implications for the phosducin family of proteins.
M. Blaauw, J. C. Knol, A. Kortholt, J. Roelofs, Ruchira, M. Postma, A. J. W. G. Visser, and P. J. M. Van Haastert (2003)
EMBO J. 22, 5047-5057
   Abstract »    Full Text »    PDF »
A quantitative characterization of the yeast heterotrimeric G protein cycle.
T.-M. Yi, H. Kitano, and M. I. Simon (2003)
PNAS 100, 10764-10769
   Abstract »    Full Text »    PDF »
Leading the way: directional sensing through phosphatidylinositol 3-kinase and other signaling pathways.
S. Merlot and R. A. Firtel (2003)
J. Cell Sci. 116, 3471-3478
   Abstract »    Full Text »    PDF »
Eukaryotic Chemotaxis: Distinctions between Directional Sensing and Polarization.
P. Devreotes and C. Janetopoulos (2003)
J. Biol. Chem. 278, 20445-20448
   Abstract »    Full Text »    PDF »
Receptor-mediated Regulation of PI3Ks Confines PI(3,4,5)P3 to the Leading Edge of Chemotaxing Cells.
Y. E. Huang, M. Iijima, C. A. Parent, S. Funamoto, R. A. Firtel, and P. Devreotes (2003)
Mol. Biol. Cell 14, 1913-1922
   Abstract »    Full Text »    PDF »
Light Microscopy Techniques for Live Cell Imaging.
D. J. Stephens and V. J. Allan (2003)
Science 300, 82-86
   Abstract »    Full Text »    PDF »
Seeing the Light: Preassembly and Ligand-Induced Changes of the Interferon {gamma} Receptor Complex in Cells.
C. D. Krause, E. Mei, J. Xie, Y. Jia, M. A. Bopp, R. M. Hochstrasser, and S. Pestka (2002)
Mol. Cell. Proteomics 1, 805-815
   Abstract »    Full Text »    PDF »
Polarization of Myosin II Heavy Chain-Protein Kinase C in Chemotaxing Dictyostelium Cells.
H. Rubin and S. Ravid (2002)
J. Biol. Chem. 277, 36005-36008
   Abstract »    Full Text »    PDF »
A Functional Link between Glucokinase Binding to Insulin Granules and Conformational Alterations in Response to Glucose and Insulin.
M. A. Rizzo, M. A. Magnuson, P. F. Drain, and D. W. Piston (2002)
J. Biol. Chem. 277, 34168-34175
   Abstract »    Full Text »    PDF »
Modeling the Cell's Guidance System.
P. A. Iglesias and A. Levchenko (2002)
Sci. STKE 2002, re12
   Abstract »    Full Text »    PDF »
A Novel Strategy to Engineer Functional Fluorescent Inhibitory G-protein {alpha} Subunits.
J. L. Leaney, A. Benians, F. M. Graves, and A. Tinker (2002)
J. Biol. Chem. 277, 28803-28809
   Abstract »    Full Text »    PDF »
A Highly Effective Dominant Negative {alpha}s Construct Containing Mutations That Affect Distinct Functions Inhibits Multiple Gs-coupled Receptor Signaling Pathways.
C. H. Berlot (2002)
J. Biol. Chem. 277, 21080-21085
   Abstract »    Full Text »    PDF »
Neutrophils lacking phosphoinositide 3-kinase {gamma} show loss of directionality during N-formyl-Met-Leu-Phe-induced chemotaxis.
M. Hannigan, L. Zhan, Z. Li, Y. Ai, D. Wu, and C.-K. Huang (2002)
PNAS 99, 3603-3608
   Abstract »    Full Text »    PDF »
Fluorescent Histochemical Techniques for Analysis of Intracellular Signaling.
M. P. Oksvold, E. Skarpen, J. Widerberg, and H. S. Huitfeldt (2002)
Journal of Histochemistry & Cytochemistry 50, 289-303
   Abstract »    Full Text »    PDF »
Adenylyl Cyclase Functions Downstream of the G{alpha} Protein Gpa1 and Controls Mating and Pathogenicity of Cryptococcus neoformans.
J. A. Alspaugh, R. Pukkila-Worley, T. Harashima, L. M. Cavallo, D. Funnell, G. M. Cox, J. R. Perfect, J. W. Kronstad, and J. Heitman (2002)
Eukaryot. Cell 1, 75-84
   Abstract »    Full Text »    PDF »
Real-Time Visualization of a Fluorescent G{alpha}s: Dissociation of the Activated G Protein from Plasma Membrane.
J.-Z. Yu and M. M. Rasenick (2002)
Mol. Pharmacol. 61, 352-359
   Abstract »    Full Text »    PDF »
Kinetic Analysis of M2 Muscarinic Receptor Activation of Gi in Sf9 Insect Cell Membranes.
V. A. Mosser, I. J. Amana, and M. I. Schimerlik (2002)
J. Biol. Chem. 277, 922-931
   Abstract »    Full Text »    PDF »
G{alpha}i3 binding to calnuc on Golgi membranes in living cells monitored by fluorescence resonance energy transfer of green fluorescent protein fusion proteins.
T. S. Weiss, C. E. Chamberlain, T. Takeda, P. Lin, K. M. Hahn, and M. G. Farquhar (2001)
PNAS 98, 14961-14966
   Abstract »    Full Text »    PDF »
Functional expression and FRET analysis of green fluorescent proteins fused to G-protein subunits in rat sympathetic neurons.
V. Ruiz-Velasco and S. R Ikeda (2001)
J. Physiol. 537, 679-692
   Abstract »    Full Text »    PDF »
The CC Chemokine Eotaxin (CCL11) Is a Partial Agonist of CC Chemokine Receptor 2b.
R. Martinelli, I. Sabroe, G. LaRosa, T. J. Williams, and J. E. Pease (2001)
J. Biol. Chem. 276, 42957-42964
   Abstract »    Full Text »    PDF »
Allurin, a 21-kDa sperm chemoattractant from Xenopus egg jelly, is related to mammalian sperm-binding proteins.
J. H. Olson, X. Xiang, T. Ziegert, A. Kittelson, A. Rawls, A. L. Bieber, and D. E. Chandler (2001)
PNAS 98, 11205-11210
   Abstract »    Full Text »    PDF »
Reducing the Environmental Sensitivity of Yellow Fluorescent Protein: MECHANISM AND APPLICATIONS.
O. Griesbeck, G. S. Baird, R. E. Campbell, D. A. Zacharias, and R. Y. Tsien (2001)
J. Biol. Chem. 276, 29188-29194
   Abstract »    Full Text »    PDF »
Reducing the Environmental Sensitivity of Yellow Fluorescent Protein: MECHANISM AND APPLICATIONS.
O. Griesbeck, G. S. Baird, R. E. Campbell, D. A. Zacharias, and R. Y. Tsien (2001)
J. Biol. Chem. 276, 29188-29194
   Abstract »    Full Text »    PDF »
Kinetic Analysis of M2 Muscarinic Receptor Activation of Gi in Sf9 Insect Cell Membranes.
V. A. Mosser, I. J. Amana, and M. I. Schimerlik (2002)
J. Biol. Chem. 277, 922-931
   Abstract »    Full Text »    PDF »
Allurin, a 21-kDa sperm chemoattractant from Xenopus egg jelly, is related to mammalian sperm-binding proteins.
J. H. Olson, X. Xiang, T. Ziegert, A. Kittelson, A. Rawls, A. L. Bieber, and D. E. Chandler (2001)
PNAS 98, 11205-11210
   Abstract »    Full Text »    PDF »

To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882